1. Preparation and Characterization of Beta-glucan/silica Nanocomposites
Jung Ju Kim*1, Ji Hwa Beak2, Yangkyu Ahn1
1Department of Nanochemistry & Biochemistry, Konyang University
Nonsan, Chungnam, Korea
2Department of chemistry, Konyang University

2. Abstract DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY
A new class of beta-glucan/silica nanocomposites using a silica sol from water glass was successfully prepared by the sol-gel process. Silica particles  with diameter of around micrometer were observed by optical microscopy and scanning electron microscopy. FT-IR analyses confirmed that the composite microsphere was a segmented hybrid material composed of the beta-glucan segment and silica. The physicochemical properties such as morphological change by degradation, beta-glucan loading efficiency, and beta-glucan release profiles were investigated with the beta-glucan/silica nanocomposite microspheres. The speed of degradation and beta-glucan release decreased with increasing silica content. 
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

3. Objectives of this study
Synthesis of decrease the molecular weight method of beta-glucan
Synthesis of beta-glucan microsphere using crosslinking reaction
Control of the size of the beta-glucan particles
Synthesis of biopolymer-coated durgs microspheres
for biomedical application
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

4. <structure of β-1,3-1,6-glucan>
What is β-glucan?
<structure of β-1,3-1,6-glucan>
Safety of beta-glucan
Beta-1,3-D glucan   ㆍis a natural product   ㆍhas been part of the human diet for thousands of years.   ㆍis permitted for use in food by the U.S. FDA (GRAS)   * GRAS : Generally Recognized As Safe
Functional of beta-glucan
- Immunotherapy of cancer - Immunity boosting - Counteraction of side-effects caused by cancer therapy - Prevention of infection - Wound healing &Skin regeneration - Cholesterol lowering - Recovery after radiation exposure
character of beta-glucan
- extraction in mushroom
- Molecular form : (-C6H10O5-)n
- Dissolved in alkali solution (NaOH solution)
- Gelation in acid solution (acetic acid)
Viscosity
Emulsification activity
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

5. Health assistance food and cosmetic
Uses of β-glucan
A field of study
Use
Medical supplies
immunity reinforce
Reinforcement of phagocytosis of macrophage to tuberculosis bacilli
- resistance increase of a host
Health assistance food and cosmetic
immunity reinforcement material
blood sugar and reinforcing agent of blood sugar
- regrowth of the skin and anti-aging
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

6. Molecular structures is β-Glucan
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

7. Synthesis of the molecular weight method of beta-glucan))
Concentration of
Beta-glucan solution ))
Formic acid
80~90℃
Beta-glucan
1% beta-glucan
2% beta-glucan .
6% beta-glcuan
7% beta-glucan
1% beta-glucan
2% beta-glucan .
6% beta-glcuan
7% beta-glucan
optimum conditions:
solution in formic acid
irradiation time : 20min
irradiation temperature :
keeping 80 ~ 90℃
temperature of Formic acid
Under 80 ℃
Between 80 ℃ and 100 ℃
Over 100 ℃
Indissoluble
Soluble
evaporation of formic acid
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

8. + Synthesis of beta-glucan microsphere using crosslinking reaction
Volume of Span80 :
1ml, 2ml,···, 6ml, 7ml
add glutaraldehyde
iso-octane 35ml
+ span 80 5ml )) )) +
Emulsification
add Norfloxacin
irradiation time : 20min
irradiation time : 60min
beta-glucan solution
beta-glucan using
crosslinking reaction
Acetone
+Alcohol(5:5)
optimum conditions:
solution in formic acid 5㎖
add durg : Norfloxacin
irradiation time : 20min
irradiation temperature : keeping 80 ~ 90℃
optimum conditions:
solution in formic acid 5㎖
add durg : Norfloxacin
irradiation time : 20min
irradiation temperature : keeping 80 ~ 90℃
washing
by centrifuge
freeze drying
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

9. Size distribution of beta-glucan by concentration of beta-glucan1% 2% 3% 4% 5% 6% 7%
Beta-glucan particle size (㎛)
0.252 (100%)
0.380 (87.9%)
0.500 (91.9%)
0.553 (85.4%)
0.913 (83.1%)
1.271 (92.2%)
1.979 (69.7%)
S.Dev (nm/%)
0.047
0.116
0.172
0.308
0.445
0.252
0.911
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

10. Beta-glucan particle size (㎛)
Size distribution of beta-glucan microsphere
by volume of sulfactant
Surfactant
Concentant
(vol.%)
Beta-glucan particle size (㎛)
S.Dev
(nm / %)
0.079
0.416
0.089/18.07
0.103
0.747
0.425/10.43
0.146
1.335
0.589/14.95
0.167
2.827
1.355/4.92
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

11. SEM image of beta-glucan was made to low molecule
SEM image of before crosslinking reaction and after crosslinking reaction
Before decrease the molecular weight method
Before crosslinking reaction
After decrease the molecular weight method
After crosslinking reaction
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

12. Beta-glucan image by sol-gel method
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

13. Drug loading efficiency
of beta-glucan
Expression of drug loading efficiency of beta-glucan
Drug contents :
(volume of drugs within the microsphere)
volume of drugs of microsphere
+ volume of beta-glucan
×100
ultrasonicator
micro tip
Beta-glucan microsphere 2㎎
Ethanol 10㎖
measurement : using UV spectrophotometer
absorption of UV spectrophotometer : 205nm
irradiation temperature : keeping 37.0±0.5℃
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

14. Conclusions
Beta-glucan of high polymer was made to low molecule
using treat by acid
The beta-glucan microsphere were coated dugs
by cross-linked method
The size of particles was different by volume of surfactant
According to the concentration of beta-glucan increases,
the size of beta-glucan microsphere increases too
According to the concentration of beta-glucan increases,
The content of a medicine increased
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY

15. Beta-glucan image by sol-gel method
DEPARTMENT OF NANOCHEMISTRY & BIOCHEMISTRY KONYANG UNIVERSITY