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Erin Osborne Nishimura June 5, 2017 Updated August 22, 2017

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Presentation on theme: "Erin Osborne Nishimura June 5, 2017 Updated August 22, 2017"— Presentation transcript:

1 Erin Osborne Nishimura June 5, 2017 Updated August 22, 2017
Intro to the Lab Erin Osborne Nishimura June 5, 2017 Updated August 22, 2017

2 Today’s talk Welcome to the lab Staying safe in the lab
Staying organized in the lab Staying happy in the lab

3 Welcome to the lab

4 Lab events Lab meetings Standing meetings Journal clubs
Wednesdays at 11am, MRB 316 Everyone expected to attend Standing meetings Schedule your with Erin Journal clubs Fridays at 3pm, MRB230 Grads, senior scientists expected to attend Undergrads and volunteers optional but encouraged Upcoming Symposia Q-Bio, GAUSSI, Biochemistry Department Retreat, Rocky Mountain Computing, IGAF

5 Digital Lab Resources Lab webpage Pangea Quartzy EON-NAS LabArchives
Onishlab.colostate.edu Onishlab.colostate.edu/log-in Pangea Inventories, protocols, purchases, lab meetings Quartzy ordering EON-NAS Data, images, sequencing, & backups LabArchives Electronic lab notebook

6 Staying safe in the lab

7 Chemicals and hazardous materials
Receive proper training Stay informed about chemicals and their toxicity Use appropriate personal protective equipment Lab coat Lab goggles Wear appropriate clothes Close-toed shoes Full length pants Long hair tied back Use fume hood for hazardous and stinky chemicals Phenol, chloroform, formamide, formaldehyde, etc. Label all chemicals properly Full names (ethanol) Hazardous chemicals must be in cabinets and secondary containers Get certified – log into ehs.colostate.edu and take “Hazardous Waste Training” and other relevant training like “Formaldehyde Training” Switch hazards signs on the door Look into lab coat washing Phenol smell

8 Hazardous waste disposal
Secondary containers All hazardous chemicals must be in secondary containers that can hold their full contents if the primary container fails Weekly inspections Marc performs weekly hazardous waste inspections Disposal after 90 days You can collect hazardous waste in a container in the lab for 90 days; After that point, you must request to remove it (talk to Marc) Chemical solid waste Evaporate small amounts of hazardous residue before disposal

9 Other lab hazards Glass Razors Liquid nitrogen, dry ice Centrifuges
Use glass waste bin (cardboard) Razors Keep in styrofoam Avoid using excessive force Liquid nitrogen, dry ice Open immediately or risk exploding tubes Centrifuges Always balance Microscope light sources Don’t look directly at the light Gel Red Don’t touch it

10 Leaving the lab at the end of the day
Turn off all bunsen burners & other flames Turn off all heated stir plates & heated equipment Turn off all microscope lamps Check around if you are the last person to leave

11 Staying organized in the lab

12 Stay organized in the lab
Follow protocols “Your protocol must be in your hand” – Kohta Ikegami Take notes Date everything Keep personal inventories wEN001 – Erin’s worms pEN001 – Erin’s plasmids oEN001 – Erin’s oligos Contribute to lab inventories

13 Lab notebook Everyone must have a paper lab notebook
Digital Lab notebook is available for regular lab members through LabArchives

14 Digital files – backing up
3 copies across two locations Work locally AND backup to EON-NAS (RAID5, 30-day versioning) OR work on EON-NAS and backup locally Third location currently up to you Options coming soon Automated sync

15 Staying happy in the lab

16 Being courteous Noise & talking Headphones & music Cleaning up
Be mindful of other people’s concentration when talking & making noise Headphones & music Headphones are best during business hours; blast it at night or on the weekends; everyone has music veto power Cleaning up Do we need lab chores? Shared jobs __ Autoclave waste __ dishwashing __ tip & tube autoclaving __ LB & NGM __ spatulas (buy more) __ M9 & liquid LB __ Antibiotic box Buy more spatulas

17 Being hygienic Keep enzymes in coolers
Use sterile, clean technique when using stocks and enzymes No gloves in the hallways

18 Re-order! Don’t let things run out. Use Quartzy

19 Thanks for making the lab an enjoyable place to work!

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