1. Viral and Cellular Genomes Activate Distinct DNA Damage Responses
Govind A. Shah, Clodagh C. O’Shea 
Cell 
Volume 162, Issue 5, Pages (August 2015)
DOI: /j.cell
Copyright © 2015 Elsevier Inc. Terms and Conditions

2. Figure S6
The Induction of Cellular DNA Damage by Genotoxic Drugs Prevents MRN Recruitment to Viral Genomes and the Activation of a Localized ATM Response that Restricts Viral Replication, Related to Figure 6
(A) A549 cells were infected with the indicated viruses. At 6 h.p.i., etoposide was added at 0, 3.3, 10, or 30 μg/ml to ΔE1B-55K/ΔE4-ORF3 infected cells or at 0 or 30 μg/ml to WT virus infected cells. Doxorubicin treatment was used as a positive control for cellular DNA damage. Protein lysates were collected at 24 h.p.i. and immunoblotted as indicated.
(B and C) SAECs were uninfected or infected with the indicated viruses. At 4 h.p.i., etoposide at 0, 6, 30, or 150 μg/ml or doxorubicin at 0, 0.1, or 0.5 μg/ml was added to ΔE1B-55K/ΔE4-ORF3 infected cells. Protein lysates and total DNA were harvested at 36 h.p.i. Uninfected cells were treated with etoposide at 0, 6, or 30 μg/ml or doxorubicin at 0, 0.1, or 0.5 μg/ml for 12 hr. An equal volume of DMSO was added to all cells (B) Protein lysates were immunoblotted as indicated. (C) Virus genomes were quantified by qPCR. Error bars indicate SD of quadruplicates.
(D) A549 cells were infected with the indicated viruses as in Figures 6E–6G. At 6 h.p.i., WT virus infected cells were treated with 30 μg/ml etoposide, 0.5 μg/ml doxorubicin, or 10 μM bleomycin. An equal volume of DMSO was added to all cells. Total DNA samples were collected at 24 h.p.i. Viral genomes were quantified by qPCR.
(E) A549 cells were infected with the indicated viruses. At 2 h.p.i., DMSO (-), 10 μg/ml etoposide, or 0.1 μg/ml doxorubicin were added to cells. Cells were harvested for ChIP analysis at 12 h.p.i. ChIP was performed using MRE11 antibodies. An IgG control (not shown) yielded less than 0.1% input in all samples. Virus genomes were quantified by qPCR. Fold enrichment is plotted relative to ΔE1 virus infected cells. Error bars indicate SD of quadruplicates.
Cell  , DOI: ( /j.cell )
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