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Date of download: 9/30/2017 Copyright © ASME. All rights reserved.

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1 Date of download: 9/30/2017 Copyright © ASME. All rights reserved. From: Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope J. Heat Transfer. 2015;137(9): doi: / Figure Legend: Linkam BCS-196 biological cryostage system: (a) thermal stage; (b) Dewar (liquid nitrogen); and (c) cryostage system temperature controller

2 Date of download: 9/30/2017 Copyright © ASME. All rights reserved. From: Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope J. Heat Transfer. 2015;137(9): doi: / Figure Legend: The ice crystal growth at 0.5 °C/min cooling rate in 5% DMSO solution. (a) Start freezing; (b) 10 s; (c) 20 s; (d) 30 s; (e) 40 s; (f) 50 s; (g) 60 s; and (h) 70 s.

3 Date of download: 9/30/2017 Copyright © ASME. All rights reserved. From: Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope J. Heat Transfer. 2015;137(9): doi: / Figure Legend: (a) Image of ice crystal at 0.5 °C/min cooling rate in 5% DMSO solution; (b) edge detection result by the Canny algorithm; (c) two edges expansion; (d) edge thinness; and (e) edge expansion

4 Date of download: 9/30/2017 Copyright © ASME. All rights reserved. From: Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope J. Heat Transfer. 2015;137(9): doi: / Figure Legend: Schematic illustration of calculation of the length of the ice crystal growth

5 Date of download: 9/30/2017 Copyright © ASME. All rights reserved. From: Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope J. Heat Transfer. 2015;137(9): doi: / Figure Legend: The length of the ice crystal growth in 5% DMSO, sucrose, and trehalose

6 Date of download: 9/30/2017 Copyright © ASME. All rights reserved. From: Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope J. Heat Transfer. 2015;137(9): doi: / Figure Legend: The ice crystal grew in 5% DMSO after 80 s at different cooling rate. (a) 0.5 °C/min; (b) 5 °C/min; and (c) 20 °C/min.

7 Date of download: 9/30/2017 Copyright © ASME. All rights reserved. From: Quantifying the Growth Rate and Morphology of Ice Crystals Growth in Cryoprotectants Via High-Speed Camera and Cryomicroscope J. Heat Transfer. 2015;137(9): doi: / Figure Legend: The radius of curvature (μm) of ice crystals in different cryoprotectants. (a) 5% DMSO; (b) 5% trehalose; and (c) 5% sucrose.


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