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Glycan Binding Proteins
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Glycan Binding Proteins – the latest 3D model
Y.S. Laurent (2012) Yves Saint Laurent Introduces Glycobiology,
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Glycan-Protein Recognition – Defining terms
Carbohydrate recognition domain (CRD) Carbohydrate binding determinant Lectin = Glycan Binding Protein (GBP) Glycan Sharon and Lis (1993) Scientific American
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Glycan binding protein diversity – phylogeny & function
Sharon and Lis (2004) Glycobiology 14:53R
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Essentials of Glycobiology
There are >80 mammalian lectins in at least a dozen distinct families Essentials of Glycobiology Second Edition
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C-type lectins – diverse domain structures for diverse functions
Kurt Drickamer, Imperial College London
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Animal lectins – structural classes
Lectin family Saccharide ligands Subcellular location Examples of functions Calnexin Glc1Man9 ER Protein sorting in the ER M-type lectins Man8 ER -associated degradation L-type lectins various ER, ERGIC, Golgi P-type lectins Man 6-phosphate, others Secretory pathway post-Golgi glycoprotein trafficking C-type lectins Various, calcium-dependent Cell membrane, extracellular Cell adhesion (selectins), glycoprotein clearance, innate immunity (collectins). Galectins β-galactosides Cytoplasm, extracellular cell surface crosslinking Siglecs (I-type lectins) sialic acid Cell membrane molecular & cell recognition R-type lectins Golgi, Cell membrane Enzyme targeting, hormone turnover F-box lectins GlcNAc2 Cytoplasm degradation of misfolded proteins. Ficolins GlcNAc, GalNAc cell membrane, extracellular Innate immunity. Chitinase-like lectins chito-oligosaccharides Extracellular Collagen metabolism F-type lectins fucose termini Intelectins Gal, galactofuranose, pentoses Extracellular/cell membrane Innate immunity. Fertilization and embryogenesis. Kurt Drickamer, Imperial College,
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Animal lectins – structural classes
Lectin family Saccharide ligands Subcellular location Examples of functions Calnexin Glc1Man9 ER Protein sorting in the ER M-type lectins Man8 ER -associated degradation L-type lectins various ER, ERGIC, Golgi P-type lectins Man 6-phosphate, others Secretory pathway post-Golgi glycoprotein trafficking C-type lectins Various, calcium-dependent Cell membrane, extracellular Cell adhesion (selectins), glycoprotein clearance, innate immunity (collectins). Galectins β-galactosides Cytoplasm, extracellular cell surface crosslinking Siglecs (I-type lectins) sialic acid Cell membrane molecular & cell recognition R-type lectins Golgi, Cell membrane Enzyme targeting, hormone turnover F-box lectins GlcNAc2 Cytoplasm degradation of misfolded proteins. Ficolins GlcNAc, GalNAc cell membrane, extracellular Innate immunity. Chitinase-like lectins chito-oligosaccharides Extracellular Collagen metabolism F-type lectins fucose termini Intelectins Gal, galactofuranose, pentoses Extracellular/cell membrane Innate immunity. Fertilization and embryogenesis. Kurt Drickamer, Imperial College,
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Animal lectins – cellular locations
cell membrane intracellular extracellular Dodd, R.B. and Drickamer, K. (2001) Glycobiology 11, 71R-79R
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Lectin phylogeny Kurt Drickamer, Imperial College,
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Glycan properties for molecular recognition and binding energy
Glucose Galactose Mannose Sialic Acid
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Principles of lectin-carbohydrate recognition
Precisely spaced cooperative hydrogen bonds direct or through bound water molecules Hydrophobic stacking Ionic interactions (for charged glycans) Calcium coordination (select lectins) Low site-affinity / high-avidity polyvalent binding
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A “simple” example: GlcNAc binding to wheat germ agglutinin (WGA)
Neumann, D., et al. (2004) Adv. Drug Deliv. Rev. 56, 437
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Influenza virus hemagglutinin (side and top views, arrows are sugar binding sites)
Weis et al (1988) Nature 333:426
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Sialic acid binding to influenza virus hemagglutinin
Weis et al (1988) Nature 333:426 - hydrogen bonds - hydrophobic stacking - polyvalent binding
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Siglec-1 CRD May, A.P., et al. (1998) Mol. Cell 1,
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Principles of glycan-lectin binding:
-hydrogen bonds -hydrophobic stacking -ionic interactions May, A.P., et al. (1998) Mol. Cell 1,
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-hydrophobic stacking -ionic interactions
Structural basis for sulfation-dependent self-glycan recognition by the human immune-inhibitory receptor Siglec-8 -hydrogen bonds -hydrophobic stacking -ionic interactions Pröpster et al. (2016) PNAS 113:E4170
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hydrogen bonds hydrophobic stacking calcium coordination
Weis & Drickamer (1994) Structure 2, 1227 hydrogen bonds hydrophobic stacking calcium coordination - polyvalent binding Drickamer (1997) Structure 5, 465
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Polyvalency in lectin binding – low site affinity, high polyvalent avidity
Inhibition of radiolabeled galactoside binding to intact rabbit hepatocytes The mammalian hepatic C-type lectin is a A2B trimer, each subunit having a single galactose binding site Glycan added (M) Lee et al (1983) J Biol Chem 258, 199
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Lectins were originally identified as “hemagglutinins”
Influenza “hemagglutinin”… red cells fall to the bottom unless they are crosslinked. Samples A, D, and E are active inhibition-assay/ Sugar binding specificity - inhibition of galectin-induced hemagglutination by lactose Ola et al (2007) Cell Biology International 31, 578
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Method to determine glycan binding specificity by structurally-specific reconstitution
Kelm, et al. (1994) Curr. Biol. 4, 965 Paulson JC and Rogers GN (1987) Methods Enzymol 138, 162
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Latest technology – glycan microarrays
Overlay with labeled GBP, wash, detect Stamp derivatized glycans onto glass slides Ola Blixt, Copenhagen Center for Glycomics
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Glycan microarray Stevens et al (2006) Nat Rev Microbiol 4:857
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Microarray decoding of the binding specificity of influenza hemagglutinins
Stevens, et al. (2006) Nat Rev Microbiol. 4:857-64
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Decoding the binding specificity of recent H7N9 avian/human influenza (bird flu)
Yang, et al. (2013) J Virol 87,
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Siglec-8 glycan microarray binding
6’Su-sialyl-LacNAc 6’Su-sialyl-Lex Bochner et al (2005) J Biol Chem 280:4307 & Bochner (2011) Glycan array public resource: Cyanovirin-N Ulex Europaeus Agglutinin (UEA-I) Influenza A Puerto Rico
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Plant lectins Found in a variety of legumes and other plants, often in seeds Discovered by their hemagglutinating activities Proposed to function in plant pathogen or symbiont recognition but data is not compelling Broad variety of glycan specificities - many have yet to be defined in detail Often sturdy proteins suitable for research, biotechnology and diagnostic applications
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“umbrella murder” of Georgi Markov by the Bulgarian secret police
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Plant lectin research correlation – seeking diagnostic glycosylation changes in cancer
94 different lectins arrayed for binding tissue extracted proteins Li et al (2011) Anal Chem 83:8509
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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Essentials of Glycobiology
Pathogen glycan binding proteins Viruses use glycan binding proteins (viral hemagglutinins) to infect cells Many bacterial toxins are lectins Bacteria use surface lectins to adhere to target cells Eukaryotic microbes use lectins to adhere to target cells Essentials of Glycobiology Second Edition
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Influenza virus lifecycle
Laver et al (1999) Scientific American, Jan issue, pp78-87 Linda Stannard:
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Influenza virus neuraminidase
Gubareva et al (2000) Lancet 355:827
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Rational design of an anti-influenza drug based on
sialic acid binding to the viral neuraminidase von Itzstein et al (1993) Nature 363:418
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Sialic acid-based influenza inhibitors
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Influenza virus budding in vitro without (top) and with (bottom) neuraminidase inhibitor
Peters, P.H., et al. (2001) J Am Geriatr Soc 49, 1025 Gubareva et al (2000) Lancet 355:827
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Essentials of Glycobiology
Pathogen glycan binding proteins Viruses use glycan binding proteins (viral hemagglutinins) to infect cells Many bacterial toxins are lectins Bacteria use surface lectins to adhere to target cells Eukaryotic microbes use lectins to adhere to target cells Essentials of Glycobiology Second Edition
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Bacterial AB5 toxins Cholera toxin E coli enterotoxin Pertussis toxin
Shiga toxin Shiga-like verotoxins Merritt et al (1994) Protein Science 3:166 Fishman and Brady (1976) Science 194:906
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Cholera toxin B-pentamer binding GM1 – extended glycan engagement
FIGURE Crystal structure of cholera toxin B-subunit pentamer with bound GM1 pentasaccharide shown from the bottom (a) and from the side (b). (Redrawn, with permission, from Merritt E.A., Sarfaty S., van den Akker F., L’Hoir C., Martial J.A., and Hol W.G Protein Sci. 3: 166–175.) (c) Shiga toxin pentamer with an artificial pentavalent ligand, a powerful inhibitor of the toxin. The carbohydrate ligands are shown in a ball-and-stick representation. (Dashed magenta lines) Possible conformation for the linker. (Redrawn, with permission of Macmillan Publisher Ltd., from Kitov P.I., Sadowska J.M., Mulvey G., et al Nature 403: 669–672.) Essentials of Glycobiology Second Edition Merritt et al (1994) Protein Science 3:166
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Pathogen glycan binding proteins
Viruses use glycan binding proteins (viral hemagglutinins) to infect cells Many bacterial toxins are lectins Bacteria use surface lectins to adhere to target cells Eukaryotic microbes use lectins to adhere to target cells
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Essentials of Glycobiology
Pathogen glycan binding proteins Bacteria use surface lectins to adhere to target cells Eukaryotic microbes use lectins to adhere to target cells Essentials of Glycobiology Second Edition
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Essentials of Glycobiology
Bacterial surface lectins Essentials of Glycobiology Second Edition
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E. coli binding to urinary epithelium in vitro -- specific inhibition by Gal α1-4 Gal (right)
Sharon and Lis (1993) Scientific American Jan:82
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Inhibition of Helicobacter pylori binding to human gastric epithelium by multivalent carbohydrates
Boren et al (1993) Science 262:1892
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Pathogen glycan binding proteins
Viruses use glycan binding proteins (viral hemagglutinins) to infect cells Many bacterial toxins are lectins Bacteria use surface lectins to adhere to target cells Eukaryotic microbes use lectins to adhere to target cells
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Binding of Candida glabrata to human epithelial cells
Cormack et al (1999) Science 285:578 LINK: EPA7 glycan array screening
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Glycan Binding Proteins
Objectives Learn the diversity and families of glycan binding proteins Learn the molecular/structural strategies used in protein-glycan binding and recognition Learn the methods and resources for determining glycan binding protein specificities Learn about plant lectins as tools in glycobiology research Learn about glycan binding strategies used by pathogens
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