1. Microbiology Specimen Collection Guide For UCSF Operating Rooms
August 2016
CSF
TISSUE
FLUID
SWABS
CSF bacterial culture
Bacterial culture, normally sterile site
Bacterial culture, normally sterile site
AVOID SWABS IF POSSIBLE!
Fungal culture
CMV / HSV culture
CSF fungal culture for Coccidioides
AFB culture
Molecular tests
Fungal culture
Bacterial culture, normally sterile site
AFB culture
AFB culture (approval needed)
CMV/HSV culture
CMV/HSV culture
Molecular tests
Molecular tests
Molecular tests OR OR
Sterile Container
Sterile Black Top Tube
If large
Sterile Container
Amies Charcoal Transport Medium
Keep moist with 2-3 drops of sterile saline
Anaerobic Transport Medium
Universal Transport Medium
If large
Universal Transport Medium
Sterile Black Top Tube
Sterile Black Top Tube
Place tissue on top of soft agar medium
Keep small piece moist with 2-3 drops sterile saline
Pleural, peritoneal, synovial, etc.
20 ml or more preferred
Obtain from lab
Obtain from lab
Cotton swab with wire shaft
Bacterial culture orders:
Nonsterile site
(mucosal tissues - GI, sinus, abscess, surgical drainage)
Sterile site
(normally sterile tissues and fluids)
Label sample, confirm match with requisition
Indicate source on tube and requisition
Send immediately to laboratory
Swabs not accepted for AFB or Fungal cultures