1. Self-organization of Molecular Chains and Biomedical Applications of Water Processed in Glow Discharge Plasma Irene Tereshko 1, V. Abidzina 1, V. Red’ko 1, A. Gorchakov 1, V. Tereshko 2 1 - Belarusian-Russian University, Mogilev, Belarus 2 - University of the West of Scotland, Paisley, UK

2. Objectives - Computer simulation of self-organization processes in atom and molecular chains; - Analysis of nanostructure formation processes in water chains after low-energy ion irradiation; - Biomedical applications of water proceeded in glow discharge plasma.

3. Computer simulation Morse potentialBorn-Mayer potential J and α are the parameters of dissociation energy and anharmonicity respectively; is the displacement from an equilibrium. T, a, and r are the energy constant, the shielding and atomic lengths respectively. Expanding the potentials in a Taylor series, find the interaction force: Molecular dynamics method

4. CoefficientBorn-Mayer potentialMorse potential K, N/m9,341∙10 4 1,140∙10 4 A, N/m 2 1,951∙10 15 8,244∙10 14 B, N/m 3 2,716∙10 25 3,046∙10 25 C, N/m 4 2,836∙10 35 7,980∙10 35 D, N/m 5 2,370∙10 445 3,385∙10 446 Coefficients for Morse and Born-Mayer Potentials Molecular structure of water in a solid phase. The ellipse marks a piece of 1D chain used in simulations. Computer simulation

5. x i, i = 1,…, n is displacement of i-th oscillator from the its equilibrium position, K’ is the coefficient of elasticity on the chain borders, ß and ß’ are the damping factors inside the chain and on its borders respectively. The system was solved by the Runge–Kutta method. Computer simulation For the chain of n – coupled oscillators the system of equations can be written:

6. Hydrogen Atom Chain Chain of hydrogen atoms Displacement of 50 atoms of the excited nonlinear chain at the time of stabilization. N defines the atom number in the chain.

7. H–O–H Molecule Chain Atom chain of water consisting of hydrogen and oxygen atoms. Atom displacements in the chain of H–O–H molecules versus the velocity received by the first atom. In dashed areas the collapsed chains are shown enlarged. a) chain consisting of two molecules, b) chain consisting of eight molecules.

8. 1D Water Molecule Chain 1D chain of water consisting of hydrogen and oxygen atoms. Initial and final positions of atoms of 1D water molecule after direct low-energy impact to the first atom (oxygen). Born-Mayer potential Morse potential

9. Biomedical Applications Dual-wavelength microfluorimetry analysis(abscissa and ordinate represent the luminescent intensity I530 and I640 respectively) of blood immunocytes of oncologic patient (second stage diabetes mellitus). The red and green pluses represent lymphocytes and leukocytes respectively. The white ellipses mark the distribution of fluorescent signals of lymphocytes (lower ellipse) and leukocytes (upper ellipse) in norm. a) -the state before the water treatment course; b) - after the water treatment course

10. Biomedical Applications Dual-wavelength microfluorimetry analysis(abscissa and ordinate represent the luminescent intensity I530 and I640 respectively) of blood immunocytes of oncologic patient (second stage breast cancer). The red and green pluses represent lymphocytes and leukocytes respectively. The white ellipses mark the distribution of fluorescent signals of lymphocytes (lower ellipse) and leukocytes (upper ellipse) in norm. a) -the state before the water treatment course; b) - after the water treatment course

11. Conclusions 1. Using molecular dynamics methods, the possibility of nanostructures formation in water was shown. 2. Collapse of the chains with high-energy nanocluster formation was observed in molecular and atom chains of water. 3. Significant biotrophic effects on water and biological objects processed in GDP were detected: - the crop seeds and yeast processed in GDP showed markedly greater metabolic activity compared to the control samples; - using the water and the physiological solutions processed in GDP we observed significant therapeutic effects in the test treatments of cardiovascular, oncologic and other diseases.

12. Thank you for your attention! Irene Tereshko Belarusian-Russian University Mira Avenue 43, Mogilev 212005, Belarus E-mail: iter41@mail.ru