1. Molecular Biology Fourth Edition Chapter 1 A Brief History Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.

2. 1-2 A Brief History What is molecular biology? –The attempt to understand biological phenomena in molecular terms –The study of gene structure and function at the molecular level Molecular biology is a melding of aspects of genetics and biochemistry

3. 1-3 1.1 Transmission Genetics Transmission genetics deals with the transmission of traits from parental organisms to their offspring Chemical composition of genes not known until 1944 –Gene –Phenotype

4. 1-4 Mendel’s Laws of Inheritance A gene can exist in different forms called alleles One allele can be dominant over the other, recessive, allele The first filial generation (F 1 ) contains offspring of the original parents If each parent carries two copies of a gene, the parents are diploid for that gene

5. 1-5 Mendel’s Gene Transmission Heterozygotes have one copy of each allele Parents in 1 st mating are homozygotes, having 2 copies of one allele Sex cells, or gametes, are haploid, containing only 1 copy of each gene Heterozygotes produce gametes having either allele Homozygotes produce gametes having only one allele

6. 1-6 The Chromosome Theory of Inheritance Chromosomes are discrete physical entities that carry the genes Thomas Hunt Morgan used the fruit fly, Drosophila melanogaster, to study genetics Autosomes occur in pairs in a given individual Sex chromosomes are identified as X and Y –Female has two X chromosomes –Male has one X and one Y chromosome

7. 1-7 Hypothetical Chromosomes Every gene has its place, or locus, on a chromosome Genotype is the combination of alleles found in an organism Phenotype is the visible expression of the genotype –Wild-type phenotype is the most common or generally accepted standard –Mutant alleles are usually recessive

8. 1-8 Genetic Recombination and Mapping In early experiments genes on separate chromosomes behaved independently Genes on the same chromosome behaved as if they were linked This genetic linkage is not absolute Offspring show new combinations of alleles not seen in the parents when recombination occurs

9. 1-9 Recombination During meiosis, gamete formation, crossing over can occur resulting in the exchange of genes between the two homologous chromosomes The result of the crossing-over event produces a new combination of alleles This process is called recombination

10. 1-10 Genetic Mapping Morgan proposed that the farther apart two genes are on a chromosome, the more likely they are to recombine If two loci recombine with a frequency of 1%, they are said to be separated by a map distance of one centimorgan (named for Morgan) This mapping observation applies both to bacteria and to eukaryotes

11. 1-11 Physical Evidence for Recombination Microscopic examination of maize chromosome provided direct physical observation of recombination using easily identifiable features of one chromosome Similar observations were made in Drosophila Recombination was detected both physically and genetically in both animals and plants

12. 1-12 1.2 Molecular Genetics The Discovery of DNA: The general structure of nucleic acids were found by the end of the 19 th century –Long polymers or chains of nucleotides –Nucleotides are linked by sugars through phosphate groups Composition of Genes: In 1944, Avery and his colleagues demonstrated that genes are composed of nucleic acids

13. 1-13 The Relationship between Genes and Proteins Experiments have shown that a defective gene gives a defective or absent enzyme These lead to the proposal that one gene is responsible for making one enzyme Proposal not quite correct 1.Enzyme may have several polypeptides, each gene codes for only one polypeptide 2.Many genes code for non-enzyme proteins 3.End products of some genes are not polypeptides

14. 1-14 Activities of Genes Genes perform three major roles Replicated faithfully Direct the production of RNAs and proteins Accumulate mutations thereby allowing evolution

15. 1-15 Replication Franklin and Wilkins produced x-ray diffraction data on DNA, Watson and Crick proposed that DNA is double helix –Two DNA strands wound around each other –Strands are complementary – know the sequence of one, automatically know the sequence of the other Semiconservative replication keeps one strand of the parental double helix conserved in each of the daughter double helices

16. 1-16 Genes Direct the Production of Polypeptides Gene expression is the process by which a gene product is made Two steps are required –Transcription: copy of DNA is transcribed into RNA –Translation: the RNA copy is read or translated to assemble a protein –Codon: a sequence of 3 nucleic acid bases that stand for one amino acid

17. 1-17 Genes Accumulate Mutations Genes change in several ways Change one base to another Deletions of one base up to a large segment Insertions of one base up to a large segment As the change is more drastic, it is more likely that the gene or genes involved will be totally inactivated

18. 1-18 1.3 The Three Domains of Life Current research theories support the division of living organisms into three domains 1. Bacteria 2. Eukaryota 3. Archaea living in the most inhospitable regions of the earth Thermophiles tolerate extremely high temperatures Halophiles tolerate very high salt concentrations Methanogens produce methane as a by-product of metabolism