1. Metabolism - Chapter 5 5 tasks: 1.Bringing nutrients into the cell 2.Breakdown nutrients into useable forms - catabolism 3.Make the building blocks the cell needs - biosynthesis 4.Make the building blocks into macromolecules - anabolism 5.Make new / replace cell parts

2. Getting molecules into the cell

4. Membrane transport

5. Getting molecules into the cell - group translocation

6. Metabolism = sum of catabolic and anabolic

7. Biosynthesis: take monomers

8. Make macromolecules

9. Biosynthesis of macromolecules: Dehydration synthesis ATP

10. Synthesis of macromolecules monomerpolymertype of bond

11. Catabolic pathways

13. Catabolic pathways: Glycolysis

14. Catabolic pathways: TCA cycle

15. Catabolic pathways: Pentose Phosphate Pathway

16. We still need more ATP for the cell

17. Generate energy through chemiosmosis and electron transport

18. Aerobic Respiration

19. Anaerobic respiration

20. Fermentation

21. Microbial Fermentation

22. Comparison

23. Who lives where? strict aerobe strict anaerobe aerotolerant anaerobe facultative anaerobe mircoraerophile

24. Nutritional classes of microorganisms Heterotrophs Autotrophs Chemotrophs Phototrophs

25. Nutritional classes of microorganisms carbon from CO 2

26. Autotrophs

27. Phototrophs

28. Chemotrophs

29. Regulation of metabolism Regulation of gene expression Regulation of enzyme activity

30. Enzymes

32. Enzymes - allosteric regulators

33. Enzymes - feedback inhibition

34. Genetics of Microorganisms Chapter 6

35. DNA the genetic material of all cells

36. DNA (a polymer of nucleotides)

37. Roles of DNA

38. DNA Replication

40. DNA replication

41. DNA Replication

42. DNA replication

43. Bacterial chromosomes

44. Transcription

50. Translation

53. 3 types of RNA

54. Translation

57. Genotype vs. Phenotype

58. Transcription and Translation happen simultaneously in prokaryotes

59. What about eukaryotes

60. Gene Expression

61. Regulation of genes inducible repressible constitutive

62. Operons

63. lac operon

64. lac operon - off / repressed

65. lac operon - on / induced

66. Attenuation - Histidine operon

67. Attenuation - Tryptophan operon

68. Cells sense their environment: Two component regulation

69. The genetics of prokaryotes Genome vs. plasmid

70. Why do we care about plasmids?

71. Conjugation

73. Transformation

74. Transduction

75. What about mutations?

76. What causes mutations?

80. Mutations can result in …

81. Looking for mutants in the lab

82. Screening for potential mutagens: Ames Test

83. Nosocomial infections and the rise of drug resistance