1. HYDROFILTRAT MENYANTHES G Antioxidant new generation for firming application

2. The Concept Fight against sagging and re- shape the oval of your face Improves and protects collagen synthesis for a lifting effect

3. BE INSPIRED Menyanthes trifoliata

4. Hydrofiltrat Menyanthes G: extracted from Menyanthes trifoliata Hydrofiltrat Menyanthes G is an aqueous extract of Menyanthes trifoliata obtained by lixiviation Menyanthes is a monotypic genus of flowering plant in the family Menyanthaceae containing the single species Menyanthes trifoliata. The name Menyanthes comes from the Greek words menyein, meaning "disclosing", and anthos, meaning "flower", in reference to the sequential opening of flowers on the inflorescence.

5. Menyanthes trifoliata: description Menyanthes trifoliata is characterized by a horizontal rhizome with alternate, trifoliate leaves. The inflorescence is an erect raceme of white flowers. Menyanthes trifoliata occurs in bogs and marshes in Asia, Europe, and North America. The North American form is often referred to as M. trifoliata var. minor Michx. It is known in English by the common names bog-bean and buckbean, and in German as Bitterklee or Fieberklee.

6. Menyanthes trifoliata: traditional uses Known in phytotherapy for its strengthening properties, the parts used are the fresh leaves and the whole plant, harvested from May till September when it’s still in flower. A symbol of calm and rest, it is said that a daily cup of Menyanthes can extend lifespan.

7. OBSERVE Oxidative stress and free radicals, What they are, what they do

8. Free radicals Molecular species with unpaired electrons : anion superoxyde for example very instable and oxidant for other molecules Trigger oxidative chain reactions called Oxidative Stress Responsible for cellular disorders which lead to premature ageing, loss of firmness, fat gain, increase in skin sensitivity…

9. Free radicals, origin and targets Origin: UV exposure Air pollution Cigarette smokes Intracellular reaction which use oxygen ex: mitochondrial respiration Goals in the skin: fibroblasts adipocytes keratinocytes Cellular targets: Membranous lipids Mitochondria DNA proteins Vitamins

10. Implication of oxidative stress in loss of firmness Vitamin C is responsible for collagen maturation as well as its transport in dermis Vitamin C is very instable and sensitive to oxidative stress When it is oxidized, Vitamin C is not functional for collagen maturation

11. In-vitro tests

12. In-vitro test: antioxidant activity Quercetin and Cranberry extracts (strong antioxidant properties) are used as positive controls Hydrofiltrat Menyanthes G shows very strong antioxidant capacities widely higher than Quercetin and Cranberry ORAC test: Developed by the American Agricultural Research Department. Evaluation of the overall antioxidant capacity of an extract. DPPH test: Antioxidant capacity related to polyphenol content (0.5% of Menyanthes trifoliata extract)

13. In-vitro test: stabilisation of vitamin C and improvement of collagen synthesis Culture of fibroblasts (control) Culture of fibroblasts + Vitamin C In black, collagen fibres put in evidence by immunostaining Vitamin C stimulates the production and maturation of collagen fibres Culture of fibroblasts + Vitamin C + Oxidative Stress Culture of fibroblasts + Vitamin C and Hydrofiltrat Menyanthes G 2% + Oxidative Stress Hydrofiltrat Menyanthes G protects Vitamin C from oxidation and therefore restores the maturation and exportation of collagen fibres in dermis Vitamin C + oxydative stress= solution of Vitamin C prepared the day before using

14. REVEAL Clinical test

15. Clinical test: Hydrofiltrat Menyanthes G 2% Cutometry test Protocol: Twice daily application on the face and the neck during 28 days 15 volunteers between 46 and 57 years old Measure of the firming effect ( in Uf parameter) and the toning effect ( in the Ur parameter) of the skin by cutometry on the cheeks Results: Increase in skin firmness up to 36% (-14.6% on average) Increase in skin tone up to 49% (-19.7% on average) Firming effect observed by 78% of the volunteers *-15% *p=0.025 Student test *-20% - 36% - 49%

16. Clinical test: Hydrofiltrat Menyanthes G 2% Fringe projection Significant reduction of the facial oval volume: up to 18% (-3% on average, p<0.05) observed by 80% of the volunteers

17. Hydrofiltrat Menyanthes G 2% Firming effect Strong antioxidant capacities Protects Vitamin C Improves collagen synthesis Firms the skin and improves the oval of the face Synergetic effect with Vitamin C derived ingredients Preservative free, Ecocert approved INCI: Glycerine (and) Water (and) Menyanthes trifoliata extractINCI: Glycerine (and) Water (and) Menyanthes trifoliata extract % recommended : 2%

18. Press Book

20. Example of Finished Products REDERMINE Firming Wrinkle Repair Serum Darphin / Estee Lauder Predermine, a firming wrinkle repair serum, is Darphin’s newest addition to its collection of anti-aging skincare products. The serum, formulated with the brand’s Smart Firming System, reduces the appearance of fine lines and wrinkles, as well as restores elasticity to the skin. The formulation also includes ingredients such as iris, menyanthes trifoliate, dill, soy in addition to peptides which work to regenerate the natural production of proteins. The serum is recommended for all skin types exhibiting loss of firmness and wrinkles. It can be used both morning and night, alone or with a moisturizer, and under a mask depending on your skin’s needs and issues.