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Activation of HER4 by heparin ‐ binding EGF ‐ like growth factor stimulates chemotaxis but not proliferation by Klaus Elenius, Subroto Paul, Genève Allison,

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Presentation on theme: "Activation of HER4 by heparin ‐ binding EGF ‐ like growth factor stimulates chemotaxis but not proliferation by Klaus Elenius, Subroto Paul, Genève Allison,"— Presentation transcript:

1 Activation of HER4 by heparin ‐ binding EGF ‐ like growth factor stimulates chemotaxis but not proliferation by Klaus Elenius, Subroto Paul, Genève Allison, Jilin Sun, and Michael Klagsbrun EMBO J. Volume 16(6):1268-1278 March 15, 1997 ©1997 by European Molecular Biology Organization

2 Cross ‐ linking of [125I]HB ‐ EGF to cells expressing HER1, HER2, HER3 or HER4 alone. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

3 Cross ‐ linking of [125I]HB ‐ EGF, [125I]EGF or [125I]ARIA ‐ 1 to cells expressing HER1 or HER4. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

4 Scatchard analysis of [125I]EGF, [125I]HB ‐ EGF and [125I]HRG–β1 binding to NIH 3T3 cells expressing HER1 or HER4. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

5 Tyrosine phosphorylation of EGF receptors in cells expressing HER1 or HER4. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

6 Tyrosine phosphorylation of EGF receptors in MDA ‐ MB ‐ 453 cells. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

7 Tyrosine phosphorylation of EGF receptors in rat astrocytes. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

8 Proliferation of cells expressing HER1 or HER4 in response to growth factors. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

9 Dose ‐ dependent chemotaxis of cells expressing HER1 or HER4. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

10 Ligand ‐ stimulated PI3 ‐ K activity. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization

11 Wortmannin inhibition of chemotaxis. Klaus Elenius et al. EMBO J. 1997;16:1268-1278 ©1997 by European Molecular Biology Organization


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