1. Down-Regulation of miRNAs by Mutated FOXP2 Ellen Korcovelos May 11, 2016 BNFO 300

2. Issue at hand Dementia NeurodegenerationMemory LossSpeech Deficiencies Alzheimer’s Disease Mild Cognitive Impairment Frontotemporal Dementia Primary Progressive Aphasia Vascular Dementia

3. miRNAs and dementia RISC mRNA miRNA Mature miRNA RISC = RNA- induced silencing complex

4. miRNAs and dementia RISC mRNA miRNA Mature miRNA mRNA decaygene expression

5. miRNAs and dementia RISC mRNA miRNA Mature miRNA BACE1 APP FOXP2

6. miRNAs and dementia RISC mRNA miRNA Mature miRNA APP Aβ42

7. miRNAs and dementia RISC mRNA miRNA Mature miRNA APP Aβ42 FOXP2 ?

8. My experiment Cell Preparation Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2 Extracting the miRNA Buffer lyses tissue DNA removed by centrifugation RNA binding membrane Analyzing Expression TaqMan Low-Density Microarray Rt-PCR ?

9. My experiment Cell Preparation Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2 Extracting the miRNA Buffer lyses tissue DNA removed by centrifugation RNA binding membrane Analyzing Expression TaqMan Low-Density Microarray Rt-PCR ?

10. My experiment Cell Preparation Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2 Extracting the miRNA Buffer lyses tissue DNA removed by centrifugation RNA binding membrane Analyzing Expression TaqMan Low-Density Microarray Rt-PCR ?

11. My experiment Cell Preparation Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2 Extracting the miRNA Buffer lyses tissue DNA removed by centrifugation RNA binding membrane Analyzing Expression TaqMan Low-Density Microarray Rt-PCR ?

12. My experiment Cell Preparation Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2 Extracting the miRNA Buffer lyses tissue DNA removed by centrifugation RNA binding membrane Analyzing Expression TaqMan Low-Density Microarray Rt-PCR ?

13. Possible Results

14. Thank you.