1. Principles of Bioinorganic Chemistry - 2004

2. Zin-Naphthopyr 1, A Tautomeric Probe for Ratiometric Zinc Sensing ZNP1 Chang, et al., Proc. Natl. Acad. Sci. USA 2004, 101,1129-1134

3. Characterization of ZNP1 Fluorescein-like Em = 528 nm Naphthofluorescein-like Em = 624 nm K d = 0.55 nM Response F 624 /F 528 Chang, et al., Proc. Natl. Acad. Sci. USA, 2004, 101,1129-1134

4. Ratiometric Imaging in Live Mammalian Cells COS-7 cells Exc 488 nm, ratio imaging 624 nm/528 nm ZNP1 affords ratiometric Zn 2+ sensing in live mammalian cells ZNP1-Ac (20  M)+ TPEN (100  M)+ Zn(pyrithione) 2 (50  M)

5. Ratiometric Imaging of Neuronal [Zn 2+ ] Chang ZNP1-Ac (20  M)+ TPEN (100  M)+ Zn(pyrithione) 2 (50  M) ZNP1 affords ratiometric Zn 2+ sensing in live neurons embryonic rat hippocampal neurons, DIV 18 Exc 488 nm, ratio imaging 624 nm/528 nm

6. NO-Induced Release of Intracellular Zn 2+ Ratiometric imaging in COS cells, ex = 488 nm, ex ratio 612/526 nm ZNP1-Ac (20  M) + TPEN (2 mM)+ SNOC (10 mM) Chang, et al., Proc. Natl. Acad. Sci. USA, 2004, 101,1129-1134

7. NO-induced Release in Neurons embryonic rat hippocampal neurons, DIV 18 20  M ZNP1-Ac, 20 min, 37 °C 100  M SNOC, 37 °C Before SNOC 45 min after SNOC 15 min after SNOC Ratiometric imaging in neurons, ex = 488 nm, ex ratio 612/526 nm Chang

8. Reading Resources General References: R. S. Drago, “Physical Methods for Chemists,” 2nd ed., Surfside Scientific Publishers, Gainesville, FL (1977) L. Que, Jr., Ed., “Physical Methods in Bioinorganic Chemistry, University Science Books, Sausalito, CA (2000) Methods Described: Mössbauer Spectroscopy Electron Paramagnetic Resonance Spectroscopy XAS Optical and IR spectroscopy will be covered later; also 5.04

9. Energetics of 57 Fe Excited State Formation for Mössbauer Effet Studies http://www.webres.com/mossbauer_detail.html

10. Three Factors that Affect the Resonant Energy Chemical effects Isomer shift (  ) Quadrupolar interactions Quadrupolar splitting  E Q Externally applied H Additional splitting

11. Resonance Absorption in 57 Fe Mössbauer Spectroscopy quadrupolar splitting

12. Magnetic Splitting of Line in 57 Fe Mössbauer Spectroscopy

13. Magnetic Splitting of Line in 63/65 Cu EPR Spectroscopy

19. The Research Yard at SLAC