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AP Biology Discussion Notes Tuesday 12/09/2014
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Goals for the Day 1.Be able to describe what a photosystem is and how it works. 2.Be able to describe what is happening and how ATP is generated in the light reactions. 3.Be able to describe the importance of the photosynthetic process.
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Question of the Day 12/09 Write the 2 major stages of photosynthesis and the reactants/products of each.
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SUMMARY Chemical Equation for Photosynthesis CO 2 + H 2 O C 6 H 12 O 6 + O 2
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Light Reactions Light + H 2 O NADPH + ATP + O 2 Water supplies Electrons and Hydrogen ions that will power chemiosmosis and Phosyphorylation of ADP to ATP.
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Calvin Cycle CO 2 Carbohydrate Recycles ADP/NADP+ The MIRACLE OF LIFE! Inorganic Organic
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Species of the day 12/08 Volvox (Algae) - Volvox carteri Does this photosynthetic Algae still need to do Cellular Respiration? Explain
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Excitation of Chlorophyll by Light When a pigment absorbs light, it goes from a ground state to an excited state, which is unstable
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Figure 10.12 Excited state Heat ee Photon (fluorescence) Ground state Photon Chlorophyll molecule Energy of electron (a) Excitation of isolated chlorophyll molecule (b) Fluorescence
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A Photosystem: A Reaction-Center Complex Associated with Light-Harvesting Complexes A photosystem consists of a reaction-center complex (a type of protein complex) surrounded by light-harvesting complexes
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A Photosystem: A Reaction-Center Complex Associated with Light-Harvesting Complexes The light-harvesting complexes (pigment molecules bound to proteins) transfer the energy of photons to the reaction center
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Figure 10.13a (a) How a photosystem harvests light Thylakoid membrane Photon Photosystem STROMA Light- harvesting complexes Reaction- center complex Primary electron acceptor Transfer of energy Special pair of chlorophyll a molecules Pigment molecules THYLAKOID SPACE (INTERIOR OF THYLAKOID) ee
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Figure 10.13b (b) Structure of photosystem II Thylakoid membrane Chlorophyll STROMA Protein subunits THYLAKOID SPACE
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A primary electron acceptor in the reaction center accepts excited electrons and is reduced as a result Solar-powered transfer of an electron from a chlorophyll a molecule to the primary electron acceptor is the first step of the light reactions
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Photosystems There are two types of photosystems in the thylakoid membrane Photosystem II (PS II) functions first (the numbers reflect order of discovery) and is best at absorbing a wavelength of 680 nm The reaction-center chlorophyll a of PS II is called P680
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Figure 10.7 Gamma rays X-rays UV Infrared Micro- waves Radio waves Visible light Shorter wavelength Longer wavelength Lower energy Higher energy 380 450500 550600650 700 750 nm 10 5 nm 10 3 nm 1 nm 10 3 nm 10 6 nm (10 9 nm) 10 3 m 1 m
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Photosystem I (PS I) is best at absorbing a wavelength of 700 nm The reaction-center chlorophyll a of PS I is called P700 Photosystems
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Linear Electron Flow During the light reactions, there are two possible routes for electron flow: cyclic and linear Linear electron flow, the primary pathway, involves both photosystems and produces ATP and NADPH using light energy
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Figure 10.14-1 Primary acceptor P680 Light Pigment molecules Photosystem II (PS II ) 1 2 ee A photon hits a pigment and its energy is passed among pigment molecules until it excites P680 An excited electron from P680 is transferred to the primary electron acceptor (we now call it P680 + )
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Figure 10.14-2 Primary acceptor H2OH2O O2O2 2 H + 1/21/2 P680 Light Pigment molecules Photosystem II (PS II ) 1 23 ee ee ee P680 + is a very strong oxidizing agent H 2 O is split by enzymes, and the electrons are transferred from the hydrogen atoms to P680 +, thus reducing it to P680 O 2 is released as a by-product of this reaction
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Each electron “falls” down an electron transport chain from the primary electron acceptor of PS II to PS I Energy released by the fall drives the creation of a proton gradient across the thylakoid membrane Diffusion of H + (protons) across the membrane drives ATP synthesis
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Figure 10.14-3 Cytochrome complex Primary acceptor H2OH2O O2O2 2 H + 1/21/2 P680 Light Pigment molecules Photosystem II (PS II ) Pq Pc ATP 1 235 Electron transport chain ee ee ee 4
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Figure 10.18 STROMA (low H concentration) THYLAKOID SPACE (high H concentration) Light Photosystem II Cytochrome complex Photosystem I Light NADP reductase NADP + H To Calvin Cycle ATP synthase Thylakoid membrane 2 13 NADPH Fd Pc Pq 4 H + +2 H + H+H+ ADP + P i ATP 1/21/2 H2OH2O O2O2
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In PS I (like PS II), transferred light energy excites P700, which loses an electron to an electron acceptor P700 + (P700 that is missing an electron) accepts an electron passed down from PS II via the electron transport chain
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Figure 10.14-4 Cytochrome complex Primary acceptor H2OH2O O2O2 2 H + 1/21/2 P680 Light Pigment molecules Photosystem II (PS II ) Photosystem I (PS I ) Pq Pc ATP 1 235 6 Electron transport chain P700 Light ee ee 4 ee ee In PS I (like PS II), transferred light energy excites P700, which loses an electron to an electron acceptor
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Figure 10.14-4 Cytochrome complex Primary acceptor H2OH2O O2O2 2 H + 1/21/2 P680 Light Pigment molecules Photosystem II (PS II ) Photosystem I (PS I ) Pq Pc ATP 1 235 6 Electron transport chain P700 Light ee ee 4 ee ee P700 + (P700 that is missing an electron) accepts an electron passed down from PS II via the electron transport chain
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Each electron “falls” down an electron transport chain from the primary electron acceptor of PS I to the protein ferredoxin (Fd) The electrons are then transferred to NADP + and reduce it to NADPH The electrons of NADPH are available for the reactions of the Calvin cycle This process also removes an H + from the stroma
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Figure 10.14-5 Cytochrome complex Primary acceptor H2OH2O O2O2 2 H + 1/21/2 P680 Light Pigment molecules Photosystem II (PS II ) Photosystem I (PS I ) Pq Pc ATP 1 235 6 7 8 Electron transport chain P700 Light + H NADP NADPH NADP reductase Fd ee ee ee ee 4 ee ee
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Photosystem II Photosystem I Mill makes ATP ATP NADPH ee ee ee ee ee ee ee Photon Figure 10.15
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Cyclic Electron Flow Cyclic electron flow uses only photosystem I and produces ATP, but not NADPH No oxygen is released Cyclic electron flow generates surplus ATP, satisfying the higher demand in the Calvin cycle
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Figure 10.16 Photosystem I Primary acceptor Cytochrome complex Fd Pc ATP Primary acceptor Pq Fd NADPH NADP reductase NADP + H Photosystem II
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Some organisms such as purple sulfur bacteria have PS I but not PS II Cyclic electron flow is thought to have evolved before linear electron flow Cyclic electron flow may protect cells from light-induced damage
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A Comparison of Chemiosmosis in Chloroplasts and Mitochondria Chloroplasts and mitochondria generate ATP by chemiosmosis, but use different sources of energy Mitochondria transfer chemical energy from food to ATP; chloroplasts transform light energy into the chemical energy of ATP Spatial organization of chemiosmosis differs between chloroplasts and mitochondria but also shows similarities
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Mitochondrion Chloroplast MITOCHONDRION STRUCTURE CHLOROPLAST STRUCTURE Intermembrane space Inner membrane Matrix Thylakoid space Thylakoid membrane Stroma Electron transport chain HH Diffusion ATP synthase HH ADP P i KeyHigher [H ] Lower [H ] ATP Figure 10.17
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In mitochondria, protons are pumped to the intermembrane space and drive ATP synthesis as they diffuse back into the mitochondrial matrix In chloroplasts, protons are pumped into the thylakoid space and drive ATP synthesis as they diffuse back into the stroma
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ATP and NADPH are produced on the side facing the stroma, where the Calvin cycle takes place In summary, light reactions generate ATP and increase the potential energy of electrons by moving them from H 2 O to NADPH
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Figure 10.18 STROMA (low H concentration) THYLAKOID SPACE (high H concentration) Light Photosystem II Cytochrome complex Photosystem I Light NADP reductase NADP + H To Calvin Cycle ATP synthase Thylakoid membrane 2 13 NADPH Fd Pc Pq 4 H + +2 H + H+H+ ADP + P i ATP 1/21/2 H2OH2O O2O2
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Test Review
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