1. Top producing beamlines of the world http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html Structures credited

2. Top producing beamlines of the world http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html x10 6 unique HKLs

3. Top producing beamlines of the world http://asdp.bnl.gov/asda/Libraries/pdb_statis/latest/bml/ALL.html http://biosync.sdsc.edu/ Structures/10 20 photons

4. NumberDescriptionPercent 446028Images (~7 TB)33% 2346Data sets47% 449MAD/SAD (1:2)19% 48Published2% 8.3.1 in 2003 Beamline Efficiency

5. SecondsDescriptionPercent 104490Assigned and available91% 42093Shutter open40% 52684Collecting (3026 images)50% 51806Something else50% Beamline Efficiency “representative” 8.3.1 user

6. SecondsDescriptionPercent 51806Something else100% 247s  45 Mounting22% 229s  37 Centering16% 179s  109 Strategizing38% 309s  37 Prepping24% Beamline Efficiency “representative” 8.3.1 user

7. SecondsDescriptionPercent 16820Something else100% 10s  45 Mounting3% 30s  37 Centering7% 140s  109 Strategizing90% 0s  37 Prepping0% Beamline Efficiency “expert” user

8. Touch screen

9. Traffic light

10. firewire

11. DVD data archive

12. Beamline pager

13. Ice-B-gone

14. Foam dewars

16. Zero-parallax optics pinhole prism microscope backstop

17. Zero-parallax optics pinhole prism microscope backstop

18. Zero-parallax optics pinhole prism microscope Styrofoam™ backlight backstop

19. Zero-parallax optics pinhole prism microscope

25. Apr 6 – 24 at ALS 8.3.1 Elven Automation 27,686images collected 148datasets (15 MAD) 31investigators 56unique cells 5 KDa – 23 MDaasymmetric unit 0.94 – 32 Åresolution (3.2 Å)

26. Apr 6 – 24 at ALS 8.3.1 Elven Automation 148datasets 117succeded ~3.5 (0.1-75)hours 31failed ~61 (0-231)hours 2 / 15MAD structures

27. NumberDescriptionPercent 446028Images (~7 TB)33% 2346Data sets47% 449MAD/SAD (1:2)19% 48Published2% 8.3.1 in 2003 Beamline Efficiency

28. Hampton Pin

29. Syrrx Pin

30. plastic Pin

31. Yale Pin

32. SuperPin

33. SuperTong

34. Hampton PinSuper Tong

35. Syrrx PinSuper Tong

36. plastic PinSuper Tong

37. Yale PinSuper Tong

39. thaw Radiation Damage

40. Incremental strategy incremental_strategy.com merged.mtz auto.mat

41. Incremental strategy incremental_strategy.com merged.mtz auto.mat

42. OTIS shoot next sample

43. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

44. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

45. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

46. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

47. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

48. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

49. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

50. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

51. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

52. OTIS Experiment recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

53. OTIS recentered snapshot at 0º put away worse than 2A molecular replacement using 1ZTA using 1 Å x-rays detector at 2 Å resolution

54. OTIS recentered data set from 50º to 150º with 1º oscillations using 1 Å x-rays detector at 2.5 Å resolution