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8 and 11 April, 2005 Chapter 17 Population Genetics Genes in natural populations.

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1 8 and 11 April, 2005 Chapter 17 Population Genetics Genes in natural populations

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3 Overview Populations contain a great deal of genetic variation. If no forces act to change gene frequencies, equilibrium is reached in one generation of random mating. Mutation, selection, migration, and random sampling can change allele frequencies. Changes in frequencies of phenotypes are consequence of changes in allele frequencies at several loci.

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5 Genes in populations New alleles introduced by mutation Migration changes population composition Mating may be random or assortative and may involve inbreeding or outbreeding Recombination produces new combinations of alleles Random fluctuation in reproductive rates may result in genetic drift in allele frequencies Differential reproduction by different genotypes may result in natural selection

6 Gene frequencies Gene frequency refers to proportion of particular allelic form among all copies of gene in population Usually estimated by sampling population –diploid: 2 copies of gene homozygotes: 2 copies of allele heterozygotes: 1copy of each allele –haploid: 1 copy of allele For two alleles, p + q = 1, where p and q are frequencies of the two alleles

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8 Calculating gene frequencies Consider a sample of genotypes with the following frequencies A/A0.36 A/a 0.48 a/a 0.16 p = frequency of A = 0.36 + 0.48/2 = 0.6 q = frequency of a = 0.48/2 + 0.16 = 0.4 (note: calculations use all of the data)

9 Heterozygosity Total frequency of heterozygotes for gene in question Greatest when there are many alleles at equal frequencies Also measured from haplotypes –combinations of alleles at closely linked loci –greater than heterozygosity because two or more heterozygotes are considered

10 Polymorphism Genetic variation occurs both within and between populations Basis for evolutionary change Most common variant is wild-type Protein polymorphism –immunologic: recognized by antibodies –amino acid sequence deduced from DNA sequence differences in electrophoretic mobility

11 DNA polymorphism Chromosome polymorphism –karyotype differences –supernumerary chromosomes –translocation and inversion heterozygotes Restriction fragment length polymorphism (RFLP) –mutation creates or abolishes restriction sites, resulting in different size fragments Variable number tandem repeats (VNTR) Complete sequence variation –SNP: single-nucleotide polymorphism –variation in both coding and noncoding regions At DNA level, some variation may have no visible effect

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16 Hardy-Weinberg equilibrium In large randomly mating populations with no mutation, no migration, and no selection, allele frequencies will be in equilibrium At equilibrium, genotype and phenotype frequencies are function of allele frequencies A/A A/a a/a p 2 2pq q 2 Allele frequencies are constant over time

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18 Consequences of H-W equilibrium Most copies of rare alleles are present in heterozygotes, not homozygotes (2pq >> q 2 ) Does not apply to sex-linked genes if males and females have unequal gene frequencies Based on random mating –random with respect to genes that have no influence over mate choice –mating may be random within subgroup (endogamy) but not in population as whole

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20 Sources of variation (1) Mutation –source of genetic variation –mutation rate too low to drive evolutionary change Recombination –linkage disequilibrium nonrandom association of alleles occurs with each new mutation –repeated recombination randomizes combinations of alleles (haplotypes) linkage equilibrium

21 Sources of variation (2) Migration –immigration: migration into population –emigration: migration from population –if populations differ in gene frequencies, migration will alter frequencies Nonrandom mating –inbreeding: mating between relatives increased homozygosity –outbreeding: mating between non-relatives –positive assortative mating: mating between like individuals –negative assortative mating: mating between unalike individuals

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24 Homozygosity May result from –systematic inbreeding –continued positive assortative mating Allele may become fixed (p = 1, q = 0) in local population Same consequence may occur when new population is founded by a few individuals

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26 Selection (1) Natural selection: differential rates of reproduction and survival among different genotypes –term used by Darwin by analogy with artificial selection Darwinian fitness: relative probability of survival and rate of reproduction –consequence of relationship between phenotype and environment –same genotype may have different fitness in different environments

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29 Selection (2) Frequency-independent selection –fitness of phenotype is independent of frequency of phenotypes –fixed property of individual’s genotype Frequency-dependent selection –fitness of phenotype changes depending upon frequency of phenotype in population Fitness often measured as viability, the probability of survival to reproductive age

30 How selection works (1) Differential reproduction of different genotypes, e.g., a/a lethal at maturity genotype A/A A/a a/a q born 0.81 0.18 0.01 0.1 mature 0.81 0.18 0.00 - corrected 0.818 0.182 0.00 0.091  q = 0.091 – 0.100 = – 0.019

31 How selection works (2) Genotype a/a may not be lethal, but have reduced fitness not all offspring reach reproductive age mature genotype leaves fewer offspring Each allele may have associated fitness, W W A/A W A/a W a/a allele with highest mean fitness relative to mean of other alleles increases in frequency, others decrease

32 Well modeled behavior of a population where W(A/A) = 1, W(A/a) = 0.75 and W(a/a) = 0.4.

33 Balanced polymorphism Difficult for selection to remove already rare allele (masked in heterozygotes) When fitness of heterozygote is greater than fitnesses of homozygotes, then stable equilibrium is possible where p and q have significant frequencies –balanced polymorphism a result of overdominance Balanced polymorphism may also result from –equal fitnesses of all genotypes (selective neutrality) –introduction of new alleles by mutation and their removal by selection

34 W(ST/ST) =.89, W(ST/CH) = 1.0 and W(CH/CH) = 0.41.

35 Artificial selection Truncation selection –for continuously varying character, only individuals above or below given phenotypic value are chosen for reproduction –when repeated, called constant truncation Fitness may be reduced with increased selection

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39 Random events Genetic drift –change in gene frequency as a result of chance events from generation to generation –most effective in small populations –may result in fixation of allele (p = 1) Founder effect –small group breaks off from larger population –founding group contains sample of alleles not necessarily in same frequency as parent population May result in establishing new mutation in population even though it is selected against

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41 Assignment: Concept map, Solved Problems 1-4, All Basic and Challenging Problems.


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