1. (—THIS SIDEBAR DOES NOT PRINT—) DESIGN GUIDE This PowerPoint 2007 template produces a 36”x72” presentation poster. You can use it to create your research poster and save valuable time placing titles, subtitles, text, and graphics. We provide a series of online answer your poster production questions. To view our template tutorials, go online to PosterPresentations.com and click on HELP DESK. When you are ready to print your poster, go online to PosterPresentations.com Need assistance? Call us at 1.510.649.3001 QUICK START Zoom in and out As you work on your poster zoom in and out to the level that is more comfortable to you. Go to VIEW > ZOOM. Title, Authors, and Affiliations Start designing your poster by adding the title, the names of the authors, and the affiliated institutions. You can type or paste text into the provided boxes. The template will automatically adjust the size of your text to fit the title box. You can manually override this feature and change the size of your text. TIP: The font size of your title should be bigger than your name(s) and institution name(s). Adding Logos / Seals Most often, logos are added on each side of the title. You can insert a logo by dragging and dropping it from your desktop, copy and paste or by going to INSERT > PICTURES. Logos taken from web sites are likely to be low quality when printed. Zoom it at 100% to see what the logo will look like on the final poster and make any necessary adjustments. TIP: See if your company’s logo is available on our free poster templates page. Photographs / Graphics You can add images by dragging and dropping from your desktop, copy and paste, or by going to INSERT > PICTURES. Resize images proportionally by holding down the SHIFT key and dragging one of the corner handles. For a professional-looking poster, do not distort your images by enlarging them disproportionally. Image Quality Check Zoom in and look at your images at 100% magnification. If they look good they will print well. ORIGINAL DISTORTED Corner handles Good printing quality Bad printing quality QUICK START (cont.) How to change the template color theme You can easily change the color theme of your poster by going to the DESIGN menu, click on COLORS, and choose the color theme of your choice. You can also create your own color theme. You can also manually change the color of your background by going to VIEW > SLIDE MASTER. After you finish working on the master be sure to go to VIEW > NORMAL to continue working on your poster. How to add Text The template comes with a number of pre-formatted placeholders for headers and text blocks. You can add more blocks by copying and pasting the existing ones or by adding a text box from the HOME menu. Text size Adjust the size of your text based on how much content you have to present. The default template text offers a good starting point. Follow the conference requirements. How to add Tables To add a table from scratch go to the INSERT menu and click on TABLE. A drop-down box will help you select rows and columns. You can also copy and a paste a table from Word or another PowerPoint document. A pasted table may need to be re- formatted by RIGHT-CLICK > FORMAT SHAPE, TEXT BOX, Margins. Graphs / Charts You can simply copy and paste charts and graphs from Excel or Word. Some reformatting may be required depending on how the original document has been created. How to change the column configuration RIGHT-CLICK on the poster background and select LAYOUT to see the column options available for this template. The poster columns can also be customized on the Master. VIEW > MASTER. How to remove the info bars If you are working in PowerPoint for Windows and have finished your poster, save as PDF and the bars will not be included. You can also delete them by going to VIEW > MASTER. On the Mac adjust the Page-Setup to match the Page-Setup in PowerPoint before you create a PDF. You can also delete them from the Slide Master. Save your work Save your template as a PowerPoint document. For printing, save as PowerPoint or “Print-quality” PDF. Student discounts are available on our Facebook page. Go to PosterPresentations.com and click on the FB icon. ©2015 PosterPresentations.com 2117 Fourth Street, Unit C Berkeley CA 94710 posterpresenter@gmail.com RESEARCH POSTER PRESENTATION DESIGN © 2015 www.PosterPresentations.c om 200μL samples were taken immediately following inoculation of the new tubes and then 24 hours later for growth measurements (where more growth is indicated by a higher OD). Pre and Post readings were compared to generate weekly average growth measurements for each genotype and media. Final results were compared by t test to detect differences in average growth. Week 1: Wild-type and abz1samples were significantly different (t(17)=2.619), but no differences were found between media or individual genotypes. Week 2: The wild-type samples significantly differed by media (t(8)= 3.375) and in the SC-f media, the WT and abz1 were significantly different (t(8)=3.599); no other differences found. Week 3: The wild-type samples significantly differed by media (t(8)= 9.800). The abz1 samples also differed by media (t(8)= 2.317). No other significant differences were found. Week 4: The wild-type samples significantly differed by media (t(8)= 3.508) and in the SC-f media, the WT and abz1 were significantly different (t(8)=3.368); no other differences found. Week 5: The wild-type samples significantly differed by media (t(8)= 9.456). The abz1 samples also differed by media (t(8)= 8.103). No other significant differences were found. Week 6: The wild-type samples significantly differed by media (t(8)= 6.856). The abz1 samples also differed by media (t(8)= 2.834). No other significant differences were found. Initial aging cultures were created for wild-type and ABZ1 mutant strains from which weekly samples would be drawn. Both genotypes had three test tubes (A, B, C) each of the each media. Each week for six weeks, 5μL samples were pipetted from each aging culture tube into three new tubes of 5mL of media for optical density measurements with a plate reader. Introduction Results Past research has found dietary restrictions for model organisms will increase longevity. Specifically, reduction of folate biosynthesis has increased lifespans in C. elegans [3,5] and S. cereviseae [2,4]. Two strains of yeast were used for comparison in this experiment: wild-type (WT) and yeast with a mutation in ABZ1 (abz1). ABZ1 encodes a protein in the folate biosynthesis pathway [1]. Both strains were grown in rich medium (YPD) and minimal medium free of folate (SC-f). Based on results from previous research, it was hypothesized that the ABZ1 mutant species of S. cerevisiae would have a greater longevity than the wild type with normal folate biosynthesis pathways over the course of the experiment (six weeks). Additionally, it was hypothesized that the folate-free minimal medium would allow greater longevity in both yeast species. Materials & Methods References 1.“ABZ1” (2005, August 25). Saccharomyces Genome Database. Retrieved from http://www.yeastgenome.org/locus/S000005316/overview 2.Burtner, C. R., Murakami, C. J., Kennedy, B. K., & Kaeberlein, M. (2009). A molecular mechanism of chronological aging in yeast. Cell Cycle 8(8), 1256-1270. 3.Nguyen, T. P., & Clarke, C. F. (2012). Folate status of gut microbiome affects Caenorhabditis elegans lifespan. BMC Biology, 10(1), 66. 4.Powers, R. W., Kaeberlein, M., Caldwell, S. D., Kennedy, B. K., & Fields, S. (2006). Extensions of chronological lifespan in yeast by decreased TOR pathway signaling. Genes & Development, 20(2), 174-184. 5.Virk, B., Correia, G., Dixon, D. P., Feyst, I., Jia, J., Oberleitner, N., … & Weinkove, D. (2012). Excessive folate synthesis limits lifespan in the C. elegans: E. coli aging model. BMC Biology, 10(1), 67. Our results did not support those from previous research in C. elegans [3,5] and S. cereviseae [2,4] which indicated that reduction of folate biosynthesis has increased lifespans in these model organisms. Based on our results, the wild-type with no environmental limits on folate production will have the greatest longevity as measurable by reproductive lifespan. Conclusions Alesha Hicks, Kayla O’Connell, and Rebecca Seipelt-Thiemann Biology Department, Middle Tennessee State University Folate Effects on Lifespan of Saccharomyces cerevisiae Hypotheses Wild Type / ABZ1 SC A 123 B 123 C 123 YPD A 123 B 123 C 123 Materials & Methods While results varied week-to-week, a few trends were found: Overall, there was no difference in growth between the wild-type and ABZ1 mutant, rejecting our hypothesis that the ABZ1 mutant strain would have greater longevity. Both yeast strains had more growth in rich medium and t tests showed they grew equally well in the rich medium for the whole course of the experiment, rejecting our hypothesis that the minimal-folate free medium would allow greater longevity in both yeast species. While both strains did not grow as well in the minimal- folate free medium, wild-type and mutant growth was approximately the same. t tests showed that the ABZ1 mutant strain performed equally well in both media for most of the experiment, but at Weeks 5 and 6, rich medium was better for sustained growth. However, because these long growth assays can be subject to contamination, it will be necessary to replicate the experiment to confirm these results. Figure 2. Growth capability of aged wild-type and abz1 strains measured weekly over 6 weeks. Figure 1. Sampling diagram for aging cultures.