1. Listeria species Microscope : Cocobacilli,facultative anaerobe, require 10% Co2 motile(peritrichous flagellae),do not grow on MaConckey medium, rough colony are filamentus Species :- L. monocytogens (young animals) Visceral(septicemic)form necrotic liver foci sheep,goat,cattle neural form (circuling disease),abortion L.ivanovi abortion (sheep,cattle) pathogenesis Silage(iron rich) predispose animal infections food,milk biproducts, human infection In abbatoir cutaneous infection then generalized penetrate epithelial barriers and multiply in macrophages histopathology brain microabscess

2. Laboratory diagnosis liver,kidney,spleen B.agar or 10%CO2 CSF,brain stem,placenta selective medium 37C, 24h. Cold enrichment(12week) beta-hemolytic CAMP Motility Gram stain rough form with Rhodococcus equi with S.aureus No growth L.ivanovi(+ve) L.monocytogens(-ve) L.Monocytogens (+ve) L.ivanovi (-ve)

3. Diagnostic characters of L.monocytogens 1-Beta hemolytic 2-coccal forms 3-rough form 4-Catalase +ve (but streptococci are –ve) 5-Aesculin hydrolysis 6-CAMP test +ve (with S.aureus) 7-Motile

4. Erysiplothrix rhusiopathiae Gr+ve rods, smooth form(2.5 micron), rough form(20 micron) Catalase/oxidase (-ve), non spore forming, non motile) Diseases Pig septicaemia, ulcerated mucous membrane of stomach skin form (urticaria) polarthritis, endocarditis Turkey, birds septicaemia, arthritis Sheep arthritis

5. Laboratory diagnosis liver, kidney heart, synovial tissue smooth form rough form fine colony wide,flat colony Gram stain Gram stain Catalase, esculin(-ve) H2S ( +ve) non motile pH 8.5/10%Co2 5-42C for 48h B.agar

6. ErysiplothrixListeria - + Motility - + Esculin hydrolysis - + Catalase - + Beta hemolysis

7. Cow with uterine dicsharge turned to lab. for isolation of. a-brucella, listeria. b-camplyobacter c-a and b. d-non of them. Direct micoscopy for neural listeriosis comprise a-Gram stain. b-Z.N. stain. d-histopathology sections. We tray for up to 12 weeks for it’s isolaion “cold– enrichment” a-brucella abortus. b-S.aureus. c-listeria monocytogens. d-cory. ovis. Intraperitoneal inoculation of L.monocytogens in mice lead to. a-brain abscesse. b-liver necrotic lesion. c-straus reaction. d-macfydeon reaction. We differentiate Erysipelothrix rhusiopathiae from listeriae monocytogens. a-catalase test. b-fructose test. c-malonate test.d-H2S production. www.zutalaba.com

8. Genus Lactobacillus non pathogenic saprophytes location of bacilli milk, silage, mucous membranes Morphology : Gr+ve bacilli, non spore forming rods, non motile non capsulated milk B. agar Gr +ve rods catalase/oxidase(-ve) Species L. acidophilus L. salivaris Ph 5.8 5%Co2 37C/ 24h.

9. Actinomycetes Gr+ve branching filments,the genera include Actinomyces Nocardia Actinomyces bovis sulpher granules in granulation tissue of the Jaw of cattle ( Lumpy jaw ) bovine actinomycosis examin exudate on a slide sulpher granules with filamentous branching rods Actinomyces pyogens culture on B.agar give non hemolytic colony Nocardia astroids chronic granulomatous mastitis stain by modified Z.N.

10. Nocardiaactinomyces aerobicanaerobicIncubation + - Modified Z.N. (partially acid fast) + - Growth on SDA + - Aerial filaments + - spores soil Oral mucosareservoir

11. Genus Neisseriae Moraxella species Moraxella bovis lacrimation,corneal obesity combined with chlamydia, mycoplasma or mechanical irritation Conjunctival swab Direct microscopy by FAT culture on B.agar(37C,48h.) Gr-ve cocci beta hemolytic or coccobacillary growth on MaConckey Biochemical tests Oxidase test( +Ve ) Catalase (+ve)