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Supplementary Figures NormalCRC CRC+5FU CRC+CPT11 100µm S1.

Published byCecil Burke Modified over 8 years ago

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Presentation on theme: "Supplementary Figures NormalCRC CRC+5FU CRC+CPT11 100µm S1."— Presentation transcript:

1 Supplementary Figures NormalCRC CRC+5FU CRC+CPT11 100µm S1

2 Normal CRC CRC+ 5FU CRC+ CPT11 Gr-1 + CD11b + cells (%) ** NS Normal CRC CRC+ 5FU CRC+ CPT11 Gr-1 + CD11b + cells (%) ** S2 Normal CRC CRC+ 5FU CRC+ CPT11 CD247 (MFI) * NS Normal CRC CRC+ 5FU CRC+ CPT11 CD247 (MFI) ** NS A B

3 Normal CRC CRC+ 5FU CRC+ CPT11 CD4 + Foxp3 + cells (%) * S3

4 S4 NO- production (MFI) ** * M-MDSCsG-MDSCs MFI ** ROSNO - NS A B C ** NS CD8+ cell proliferation (%) Normal CRC CRC+ 5FU CRC+ 5FU+ CPT11 CRC+ CPT11 *** * No drug 5FU CPT11 5FU+CPT11 * M-MDSCsG-MDSCs ROS production (MFI) * 21.88% 65.06% 41.78% 19.13% 24.08% Normal CRC +5FU CRC +CPT11 CRC +5FU +CPT11 CFSE Cell count Non- activated 7.11% D * NS CD247 expression (MFI) Normal CRC CRC+ 5FU CRC+ 5FU+ CPT11 CRC+ CPT11

5 w1w2w3w4w5w6w7w8w9w10w11 Harvest AOM DSS Anti-Gr1 mAb injections S5

6 AB S6 Cleaved caspase-3 (MFI) Normal CRC CRC+ 5FU CRC+ CPT11 CRC+ 5FU+ CPT11 Cleaved caspase-3 (MFI) Normal CRC CRC+ 5FU CRC+ CPT11 CRC+ 5FU+ CPT11 Cleaved caspase-3 (MFI) ** * M-MDSCsG-MDSCs No drug 5FU CPT11 5FU+CPT11

7 CRC CRC+5FU CRC+CPT11 CRC CRC+5FU CRC+CPT11 * NS * ** S100A8 S100A9 mRNA (RQ) 5FU µmol/L mRNA (RQ) * CPT11 µmol/L NS * mRNA (RQ) S100A9 CPT11 µmol/L NS mRNA (RQ) 5FU µmol/L ** mRNA (RQ) TNFα A C B S7

8 E A Gr-1 + CD11b + cells (x10 6 ) ** NS Inflamed 5FU CPT11 - + + + + - - + - + - - - + + B CD4 + Foxp3 + cells (%) *** Inflamed 5FU CPT11 - + + + + - - + - + - - - + + S8 Relative CD247 expression (%) ** * Inflamed 5FU CPT11 - + + + + - - + - + - - - + + Relative CD247 expression (%) * * Inflamed 5FU CPT11 - + + + + - - + - + - - - + + Relative CD247 expression (%) * NS Inflamed 5FU CPT11 - + + + + - - + - + - - - + + D C CD8+ cell proliferation (%) NS Inflamed 5FU CPT11 - + + + + - - + - + - - - + + ***

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