1. Chapter 7: Cell Structure and Function 7.1: Life is Cellular Key Questions: 1)What is the cell theory? 2)How do microscopes work? 3)How are prokaryotic and eukaryotic cells different?

2. Lesson Overview Lesson Overview Life Is Cellular THINK ABOUT IT What’s the smallest part of any living thing that still counts as being “alive?” Can we just keep dividing living things into smaller and smaller parts, or is there a point at which what’s left is no longer alive? As you will see, there is such a limit. The smallest living unit of any organism is the cell.

3. Fig. 6-2 10 m 1 m 0.1 m 1 cm 1 mm 100 µm 10 µm 1 µm 100 nm 10 nm 1 nm 0.1 nm Atoms Small molecules Lipids Proteins Ribosomes Viruses Smallest bacteria Mitochondrion Nucleus Most bacteria Most plant and animal cells Frog egg Chicken egg Length of some nerve and muscle cells Human height Unaided eye Light microscope Electron microscope Though usually too small to be seen by the unaided eye, cells can be complex

4. Lesson Overview Lesson Overview Life Is Cellular Early Microscopes It was not until the mid-1600s that scientists began to use microscopes to observe living things. In 1665, Englishman Robert Hooke used an early compound microscope to look at a nonliving thin slice of cork, a plant material. Under the microscope, cork seemed to be made of thousands of tiny, empty chambers that Hooke called “cells”. The term cell is used in biology to this day. Today we know that living cells are not empty chambers, but contain a huge array of working parts, each with its own function.

5. Lesson Overview Lesson Overview Life Is Cellular Early Microscopes In Holland, Anton van Leeuwenhoek examined pond water and other things, including a sample taken from a human mouth. He drew the organisms he saw in the mouth— which today we call bacteria.

6. Lesson Overview Lesson Overview Life Is Cellular The Cell Theory Soon after Leeuwenhoek, observations made by other scientists made it clear that cells were the basic units of life. In 1838, German botanist Matthias Schleiden concluded that all plants are made of cells. The next year, German biologist Theodor Schwann stated that all animals were made of cells. In 1855, German physician Rudolf Virchow concluded that new cells could be produced only from the division of existing cells.

7. Lesson Overview Lesson Overview Life Is Cellular The Cell Theory These discoveries are summarized in the cell theory, a fundamental concept of biology. The cell theory states: -All living things are made up of cells. -Cells are the basic units of structure and function in living things. -New cells are produced from existing cells.

8. Lesson Overview Lesson Overview Life Is Cellular Light Microscopes and Cell Stains A typical light microscope allows light to pass through a specimen and uses two lenses to form an image. The first set of lenses, located just above the specimen, produces an enlarged image of the specimen. The second set of lenses magnifies this image still further. Because light waves are diffracted, or scattered, as they pass through matter, light microscopes can produce clear images of objects only to a magnification of about 1000 times.

9. Lesson Overview Lesson Overview Life Is Cellular Light Microscopes and Cell Stains Another problem with light microscopy is that most living cells are nearly transparent, making it difficult to see the structures within them. Using chemical stains or dyes can usually solve this problem. Some of these stains are so specific that they reveal only compounds or structures within the cell. Some dyes give off light of a particular color when viewed under specific wavelengths of light, a property called fluorescence. Fluorescent dyes can be attached to specific molecules and can then be made visible using a special fluorescence microscope. Fluorescence microscopy makes it possible to see and identify the locations of these molecules, and even to watch them move about in a living cell.

10. Lesson Overview Lesson Overview Life Is Cellular Electron Microscopes Light microscopes can be used to see cells and cell structures as small as 1 millionth of a meter. To study something smaller than that, scientists need to use electron microscopes. Electron microscopes use beams of electrons, not light, that are focused by magnetic fields. Electron microscopes offer much higher resolution than light microscopes. There are two major types of electron microscopes: transmission and scanning.

11. Lesson Overview Lesson Overview Life Is Cellular Electron Microscopes Transmission electron microscopes make it possible to explore cell structures and large protein molecules. Because beams of electrons can only pass through thin samples, cells and tissues must be cut first into ultra thin slices before they can be examined under a transmission electron microscope. Transmission electron microscopes produce flat, two-dimensional images.

12. Lesson Overview Lesson Overview Life Is Cellular Electron Microscopes In scanning electron microscopes, a pencil-like beam of electrons is scanned over the surface of a specimen. Because the image is of the surface, specimens viewed under a scanning electron microscope do not have to be cut into thin slices to be seen. Scanning electron microscopes produce three-dimensional images of the specimen’s surface.

13. Lesson Overview Lesson Overview Life Is Cellular Electron Microscopes Because electrons are easily scattered by molecules in the air, samples examined in both types of electron microscopes must be placed in a vacuum in order to be studied. Researchers chemically preserve their samples first and then carefully remove all of the water before placing them in the microscope. This means that electron microscopy can be used to examine only nonliving cells and tissues.

14. Fig. 6-4 (a) Scanning electron microscopy (SEM) TECHNIQUERESULTS (b) Transmission electron microscopy (TEM) Cilia Longitudinal section of cilium Cross section of cilium 1 µm

15. Different Microscopes Different microscopes reveal different aspects of this Green Algae

16. Lesson Overview Lesson Overview Life Is Cellular Prokaryotes and Eukaryotes Although typical cells range from 5 to 50 micrometers in diameter, the smallest are only 0.2 micrometers across, so small that they are difficult to see under even the best light microscopes. In contrast, the giant amoeba may be 1000 micrometers in diameter, large enough to be seen with the unaided eye as a tiny speck in pond water. Despite their differences, all cells contain the molecule that carries biological information—DNA. In addition, all cells are surrounded by a thin, flexible barrier called a cell membrane. Cells fall into two broad categories, depending on whether they contain a nucleus. The nucleus is a large membrane-enclosed structure that contains the cell’s genetic material in the form of DNA. The nucleus controls many of the cell’s activities.

17. Lesson Overview Lesson Overview Life Is Cellular Prokaryotes and Eukaryotes Eukaryotes are cells that enclose their DNA in nuclei. Eukaryotic from Greek eu (true) and karyon (kernel=nucleus Prokaryotes are cells that do not enclose DNA in nuclei. Pro Greek (before), karyon (kernel=nucleus). Generally the smallest, simplest cells

18. Lesson Overview Lesson Overview Life Is Cellular Eukaryotes Eukaryotic cells are generally larger and more complex than prokaryotic cells. Most eukaryotic cells contain dozens of structures and internal membranes. Many eukaryotes are highly specialized. There are many types of eukaryotes: plants, animals, fungi, and organisms commonly called “protists.”

19. Lesson Overview Lesson Overview Life Is Cellular Prokaryotic cells are generally smaller and simpler than eukaryotic cells. Despite their simplicity, prokaryotes grow, reproduce, and respond to the environment, and some can even move by gliding along surfaces or swimming through liquids. The organisms we call bacteria are prokaryotes. Prokaryotes

20. Bacteria: Beneficial vs. Helpful Bacteria as pathogens – plant diseases wilts, fruit rot, blights – animal diseases tooth decay, ulcers anthrax, botulism plague, leprosy, “flesh-eating” disease STDs: gonorrhea, chlamydia typhoid, cholera TB, pneumonia lyme disease Benefical Bacteria – Life on Earth is dependent on bacteria – decomposers recycling of nutrients from dead to living – nitrogen fixation only organisms that can fix N from atmosphere – needed for synthesis of proteins & nucleic acids – plant root nodules – help in digestion (E. coli) digest cellulose for herbivores – cellulase enzyme produce vitamins K & B 12 for humans – produce foods & medicines from yogurt to insulin