1. TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GENOME SCANS GDR, COMPARATIVE GENOMICS New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS BREEDERS’ TOOLBOX DEVELOPMENT Socio-economics information DNA information Efficient routine breeding operations Better fruit! STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING Overview Seedling selection scheme

2. New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit MAB PIPELINING GENOME SCANS STANDARDIZED PHENOTYPING GERMPLASM CHOICE TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS Routine MAB (marker-assisted breeding) BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

3. GENOME SCANS New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING GDR, COMPARATIVE GENOMICS TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT Marker priorities 1. Socio-economics BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

4. GENOME SCANS New QTLs discovered Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 2. Phenotyping &Germplasm (Pedigree-Based Analysis (PBA) ) PBA: crop - wide PBA: breeding program STANDARDIZED PHENOTYPING GERMPLASM CHOICE BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

5. New QTLs discovered Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 3. Genome scans GENOME SCANS PBA: crop - wide PBA: breeding program BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

6. GENOME SCANS Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 4. QTL discovery (quantitative trait loci (QTL)) New QTLs discovered PBA: crop - wide PBA: breeding program DNA information BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

7. GENOME SCANS New QTLs discovered PBA: crop-wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 5. Fast-tracked pipelining MAB PIPELINING Promising targets BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

8. PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS GENOME SCANS New QTLs discovered Socio-economics information DNA information Better fruit GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 6. “Breeders’ Toolbox” (BIMS) Efficient routine breeding operations STANDARDIZED PHENOTYPING MAB PIPELINING BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

9. New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit MAB PIPELINING GENOME SCANS STANDARDIZED PHENOTYPING GERMPLASM CHOICE TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 7. Technology Transfer BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

10. TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GENOME SCANS GDR, COMPARATIVE GENOMICS New QTLs discovered PBA: crop - wide Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE Seedling selection scheme MAB PIPELINING 8. News & Events BREEDERS’ TOOLBOX DEVELOPMENT

11. GDR Genome Database for Rosaceae

12. Abbreviation - SNP is single- nucleotide polymorphism, (pronounced snip) is a DNA sequence variation occurring when a single nucleotide A, T, C, or G — in the genome (or other shared sequence) differs between members of a biological species or paired chromosomes in an individual.DNA sequencenucleotideATCGgenomebiological specieschromosomes

13. Abbreviations - PBA Pedigree-Based Analysis (PBA) is an existing statistical framework with associated software designed to identify, validate, and use QTL information from pedigree-linked individuals to inform breeding decision-making The PBA approach exploits all data, including allelic diversity, in sets of multiple heterozygous parents and pedigreed family structure Pedigree can refer to the line age or genealogical descent of people, whether documented or not, or of animals, whether purebred or not

14. Abbreviations - MAB marker-assisted breeding (MAB) A linkage map is a genetic map of a species or experimental population that shows the position of its known genes or genetic markers relative to each other in terms of recombination frequency, rather than as specific physical distance along each chromosome. Linkage mapping is critical for identifying the location of genes that cause genetic diseases.genesgenetic markers A genetic map is a map based on the frequencies of recombination between markers during crossover of homologous chromosomes. The greater the frequency of recombination (segregation) between two genetic markers, the farther apart they are assumed to be. Conversely, the lower the frequency of recombination between the markers, the smaller the physical distance between them. Historically, the markers originally used were detectable phenotypes (enzyme production, eye color) derived from coding DNA sequences; eventually, confirmed or assumed noncoding DNAsequences such as microsatellites or those generating restriction fragment length polymorphisms (RFLPs) have been used.recombinationcrossoverhomologous chromosomesphenotypescoding DNAnoncoding DNAmicrosatellitesRFLPs

15. Abbreviations - QTL Quantitative traits refer to phenotypes (characteristics) that vary in degree and can be attributed to polygenic effects, i.e., product of two or more genes, and their environment. Quantitative trait loci (QTLs) are stretches of DNA containing or linked to the genes that underlie a quantitative trait. Mapping regions of the genome that contain genes involved in specifying a quantitative trait is done using molecular tags such as AFLP or, more commonly SNPs. This is an early step in identifying and sequencing the actual genes underlying trait variation.

16. Supplying individual changes own behaviour PBA and MAB of Innovations of market segment Buying individual at new market segment gets full service of policy and technology Technology: Calls for Markers development Policy: Persuasion to respect pedigree advantages Patent: PBA and MAB consistence proven E-journal E-learning Hormesis: Early initiation and closing of stress Antibiotics: Management and Marketing Probiotics: Programming From sensors to reflexes

17. Involvement and attitude issues Pedigree: 1.Expert 2.Moderator 3.Planner 4.Project partner 5.User Markers: 1.There is none „No“ in the text!!! 2.Cause and effects identified 3.Cause modified both general orientation and planned action 4.Repeated more detailed question to variables are proved by found values (positivistic or relativistic) 5.Partners are planning to develop values, which are not used yet 6.Experts offers to moderator further details, which are not used yet

18. Involvement and attitude issues in SMS advertising campaign Markers: 1.Shop with number of consequent a-c and source d-f markers a)Complaints; Appreciations b)None answers c)Authority (a, b, c) d)Picture (a, b, c) e)More frequent messages (a, b, c) 2. Product with (a-f) 3. Job with (a-f) Process pedigree: 1.Cause and effects identified 2.Cause modified both general orientation and planned action 3.Repeated more detailed question to variables are proved by found values (positivistic or relativistic) 4.Partners are planning to develop values, which are not used yet 5.Experts offers to moderator further details, which are not used yet

19. Routine School for Employment Studies for become an officer, teacher, physician Course for becoming welder, driver Or to be ready to handel new challenges?

20. GENOME SCANS New QTLs discovered PBA: crop - wide PBA: breeding program Breeding markers Screening services Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING OF LIVING CONDITIONS CHOICE OF POPULATION MAB PIPELINING GDR, COMPARATIVE GENOMICS TRAIT & MARKET SEGMENT TARGET ESTABLISHMENT Marker priorities 1. Differentiation of Individual BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

21. GENOME SCANS New QTLs discovered Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 2. Phenotyping & Intercultural indexes PBA of cultures PBA: individual isation STANDARDIZED PHENOTYPING OF LIVING CONDITIONS CHOICE OF POPULATION BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

22. New QTLs discovered Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 3. Scans of living conditions Scans of living conditions PBA of cultures PBA of individual isation BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

23. 3. Scans of living conditions Few collectors or hunters in wild nature Coupling farms with farmers when kicking others out to the army or employment (colonising, co- operating, precision farming) Breeders and processors employ farmers Humans are using markers, processes and tools to train self-breeding activities for individualisation = change of karma as the concept of "action" or "deed", understood as that which causes the entire cycle of cause and effect

24. GENOME SCANS Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING GERMPLASM CHOICE MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 4. QTL discovery New QTLs discovered PBA of cultures PBA of individual isation DNA information BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

25. 4. QTL discovery QTL is an early step in identifying and sequencing the actual living conditions underlying trait variation. Mapping can be measured by infrastructure, secularity, democracy, GDP descriptors Quantitative traits refer to descriptors (characteristics) that vary in degree and can be attributed to effects of different sources (synergy), i.e., product of two or more organisational types, policies, and their environment. A single-nucleotide polymorphism (SNP, pronounced snip) is a DNA sequence variation occurring when a single nucleotide — A, T, C or G — in the genome (or other shared sequence) differs between members of a biological species or paired chromosomes in an individual.

26. GENOME SCANS New QTLs discovered PBA: crop-wide PBA: breeding program Breeding markers Screening services Marker priorities Crossing scheme Trial MASS Socio-economics information DNA information Efficient routine breeding operations Better fruit STANDARDIZED PHENOTYPING OF LIVING CONDITIONS CHOICE OF POPULATION TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 5. Fast-tracked pipelining MAB PIPELINING Promising targets BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

27. 5. Fast-tracked pipelining Cause and effect targets are: ecological, territorial, global, weekend, traditional farming or slow food movements, besides urban or „I seek“ style of life Each of the targets develops own infrastructure and individuals. Do we want to skip out into own way?

28. PBA of cultures PBA: individual isation Breeding markers Screening services Marker priorities Crossing scheme Trial MASS GENOME SCANS New QTLs discovered Socio-economics information DNA information Better fruit CHOICE OF POPULATION MAB PIPELINING TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 6. “Breeders’ Toolbox” Efficient routine breeding operations STANDARDIZED PHENOTYPING OF LIVING CONDITIONS MAB PIPELINING BREEDERS’ TOOLBOX DEVELOPMENT Seedling selection scheme

29. 6. “Web 4.0s’ Toolbox” None „no“ Experience Details discovering question for plan of author Details discovering question for plans of partners General consequences discovering question for experts

30. New QTLs discovered PBA of cultures PBA: individual isation Reaction markers Screening services Marker priorities Work packages Programm es Socio-economics information DNA information Efficient routine training communic ation Better empl oyer MAB PIPELINING GENOME SCANS STANDARDIZED PHENOTYPING OF LIVING CONDITIONS CHOICE OF POPULATION TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 7. Technology Transfer BREEDERS’ TOOLBOX DEVELOPMENT Participant selection scheme

31. 7. Technology Transfer Replacement: Anti-Pro-biotics, Logical arguments vs. Reflex reactions Surprises: Boulevard Testimonials, sensory analysis, metaphors, hedonics Hotspots, pathways: improvement of weak points like CMS, yellow seeds, cis, trans, e (co-expression) Socialisation: Show them art and they will come to pay for prestige

32. New QTLs discovered PBA of cultures PBA: individual isation Reaction markers Screening services Marker priorities Work packages Programm es Socio-economics information DNA information Efficient routine training communi cation the individual is considered to be the sole doer and enjoyer of his karmas and their 'fruits' MAB PIPELINING GENOME SCANS STANDARDIZED PHENOTYPING OF LIVING CONDITIONS CHOICE OF POPULATION TRAIT & MARKET SEGMENT BREEDING TARGET ESTABLISHMENT GDR, COMPARATIVE GENOMICS 8. Innovations Assembly BREEDERS’ TOOLBOX DEVELOPMENT Participant selection scheme

33. 8. Innovations / Innovators Assembly Karma or Web 4.0 or Breeders The individual is considered to be the sole doer and enjoyer of his karmas and their 'fruits' through: remplacements, surprises, hotspots, pathways and socialisation PBA, MAB, QTL, SNP, GDR E-learning, e-journal

34. crossover, mutation, selection Evolutionary algoritm = f(crossover, mutation, selection) FEP = fast evolutionary programming Stable = distributions of random and individual variables are not different second moment http://www.eee.metu.edu.tr/~vision/books/ alife/ch5.html

35. Ethnography Lakota and Dakota Sioux describes literacy as more than reading and writing; it also includes constructions of meaning drawn from "music, dance, and art" that the children use to "make sense of their worlds" (Noll's, 1998, p. 211) African-American children often use topic-associated ways of sharing; they tell stories that share a theme or follow a person through episodes Michaels (1981) says European-American children often use topic-centered ways of sharing; their descriptions are linear, have a single or related focus, and are closed or resolved tune in carefully to the perspectives and experiences of others, and to recognize and suspend one's own assumptions the instructor should plan an active role in processing their investigations" (Boyle-Baise and Sleeter p. 380, 1996) People from oral traditions contextualize their articulation of thought; they depend on shared knowledge of the people who will be listening to them and do not necessarily articulate what others already know"; in contrast, "people from literate traditions tend to decontextualize thought, to add the context that a distant audience will need to make sense of speech or writing" (Cleary and Peacock, p. 188, 1998) Heath observed that African-American children were not asked known-answer questions at home but were expected to answer them at school. When the children did not respond in the teacher- expected manner, some teachers assumed that the children did not know the answers; in fact, the children did know the answers but did not offer to verbalize them because everyone else knew the answers as well. Orange and Horowitz's data indicates that the literacy tasks the teachers expected to be the preferences of their students were not, in fact, the students' actual preferences. The result was "an 'academic standoff' or mutual resistance, in which teachers and students each had perceptions that clearly counterbalanced those of the other" (p. 28) as each group felt the other did not care http://www.ncrel.org/sdrs/areas/issues/content/cntareas/reading/li400.htm