1. IsoXL BSL3/BSL4 SYSTEM FOR LARGER ANIMALS By Corporate Marketing Department -Tecniplast, Buguggiate -

2. WHAT IS ISOXL? First REAL biocontainment solution on the market for big animals. -Fully autoclavable -Sealed -ULPA filters -Transfer Box -Constant control of negative P -Bagging system for cage change

3. FEATURES SAFETY: possibility to carry out the whole experiment without opening the cage: - opening for bag-in/bag-out waste tray changing; - external refilling for food and water; - transfer system to move the animal under a biocontainment cabinet for procedures (e.g. take blood sample). SIZE: floor area is 4900 cm 2 and internal height is 500 mm (compliant with guidelines). DECONTAMINATION: autoclave/VHP

4. CONTAINMENT ISOXL works just in negative pressure to guarantee BSL3/BSL4 containment. Containment is guaranteed by: 2 ULPA filters, INLET and OUTLET. 1 HEPA filter on the pressure device. 1 PALL filter 0.2 μm on the watering system. 1 PRE-FILTER on the outlet.

5. ULPA FILTER INLET ULPA FILTER OUTLET PRE – FILTER HEPA FILTER BETWEEN WATER BOTTLE AND WATERING SYSTEM VALVE HEPA FILTER ON PRESSURE DEVICE BLOWER CONTAINMENT

6. Containment is guaranteed also by: Airtight replaceable silicon gasket on the main door and on the door for the removable tray. Airtight silicon gasket on the opening of the transfer box.

7. CONTAINMENT

8. Transfer box to move the animal under a biocontainment cabinet for procedures It is provided with valves to give the opportunity of anaesthetizing the animal inside without touching it (safe handling of the animal for procedures). SAFETY: transfer box

9. LOCKING SYSTEM FOR THE TRANSFER BOX It does not allow you disconnecting the TB both if the cage door and the transfer box door is open: SAFETY

10. SAFETY: pulling device This device is designed to push gently the animal into the transfer box. PROCEDURE: 1. Rotating the worm screw on the side of the cage, the operator makes a S/S panel slide towards the opposite wall. 2. In the meantime, the transfer box is opened by the operator and the ferret is guided to move into it by the sliding panel. 3. When the transfer box is closed, the gap between the tranfer box and the cage is disinfected by a spray disinfectant.

11. PULLING DEVICE

12. OPENING TO MOVE OUT THE ANIMAL It is 130 mm: EASY ACCESS FOR THE ANIMAL 130 MM

13. HEPA FILTER ON THE PRESSURE DEVICE It is easy to replace: ERGONOMIC

14. SENSORS The cage is equipped with temperature and humidity sensors: COMPLETE INTERNAL ENVIRONMENTAL MONITORING

15. OTHER FEATURES EXTERNAL FEEDER AND WATER BOTTLE OPENING FOR BAG IN – BAG OUT PROCEDURE

16. ISOXL ADVANTAGES NO CROSS - CONTAMINATION: ULPA filter at cage level; SAFETY for users: no need to open the cage and touch the animal - Transfer box. - Bag-in and bag-out procedure to change waste tray. - External feeder and bottle. NO STRESS for the animal: gentle transfer procedure and animal is not forced.

17. DECONTAMINATION Cage is completely autoclavable Electrical parts easy removable for VHP treatments ULPA FILTERS maintain their efficacy after up to 5 autoclaving cycles.

18. SUMMARIZING High dangerous studies involving different viruses BSL3/BSL4 needs specific equipments; ISOXL’s FEATURES: containment, safety,size autoclavability (bag in – bag out, transfer system, nudging system; ULPA and HEPA filters etc.); ADVANTAGES: no cross contamination, safety and no stress for the animal;

19. THANK YOU