1. WG 2A “ECOSTAT” Stresa, 3-4 July 2006 L-M GIG Final report Presented by J.Ortiz-Casas (ES), GIG coordinator Data analysis by L. Serrano and C. de Hoyos (ES)

2. Background information Common intercalibration types Common intercalibration types Three (revised) types : Three (revised) types : - CALCAREOUS (former LM 8) - CALCAREOUS (former LM 8) - SILICEOUS WET (from former LM5 and LM7) - SILICEOUS WET (from former LM5 and LM7) - SILICEOUS DRY (from former LM5 and LM7) - SILICEOUS DRY (from former LM5 and LM7)

3. L- M GIG present TYPOLOGY TYPELATITUDE ANNUAL RAINFALL/ AIR TEMP. ELEVATIONGEOLOGY 3900 (ETRS 89) 800mm 15 ºC Low-mid200-800mCalc. >1,0 meq/l Silic. 0,2-1,0 meq/l Siliceous wet >> or < xx Siliceousdr y << and > xx Calca- reous ->xx

4. LM-GIG Number of reference sites TYPECYESFRGRITPTRO Total per type Siliceous Wet -2-1-2-5 Siliceous Dry -----1-1 Calcareous121----5 Total per country 1411-3111

5. LM-GIG Number of IC (G/M) sites TYPECYESFRGRITPTRO Total per type Siliceous Wet -9---4316 Siliceous Dry -2--12-5 Calcareous271-2-416 Total per country 2181-36737

6. Background information PRESSURE: PRESSURE: Nutrient loading Nutrient loading BIOLOGICAL QUALITY ELEMENTS: BIOLOGICAL QUALITY ELEMENTS: Phytoplankton Phytoplankton * Biomass * Biomass Chlorophyll a concentration Chlorophyll a concentration Total biovolume Total biovolume * Composition * Composition % Cyanobacteria (biovolume) % Cyanobacteria (biovolume) Catalan Index Catalan Index

7. IC approach General interalibration approach General interalibration approach Option 1 Option 1 * Same metrics, same sampling strategy and same lab methods: comparability totally assured. * IC registered sites, plus reference sites, the statisitical basis for boundary setting.

8. Data Previously available data were scarce, incomplete and obtained by different sampling and lab methods. Previously available data were scarce, incomplete and obtained by different sampling and lab methods. Data collection entirely relied upon an agreed common sampling programme and laboratory methodology, applied to all IC and reference sites, and jointly performed during 2005 summer season. 3-4 sampling dates per site, euphotic layer (2.5*SD) integrated samples. Data collection entirely relied upon an agreed common sampling programme and laboratory methodology, applied to all IC and reference sites, and jointly performed during 2005 summer season. 3-4 sampling dates per site, euphotic layer (2.5*SD) integrated samples.

9. National classification methods Except FR, no national methodology for phytoplankon has been officially established at national level. Except FR, no national methodology for phytoplankon has been officially established at national level. However, we have got a common sampling strategy and lab methods agreed in the GIG for all four indices: chlorophyll a concen-tration, total biovolume, % cyanobacteria, and Catalan index. However, we have got a common sampling strategy and lab methods agreed in the GIG for all four indices: chlorophyll a concen-tration, total biovolume, % cyanobacteria, and Catalan index.

10. Setting of reference conditions Common approach. Common approach. * Spatial approach: Ref. sites selected among reservoirs of the concerned type. * Spatial approach: Ref. sites selected among reservoirs of the concerned type. * Requirements for HMWB assumed to be met. * Requirements for HMWB assumed to be met. * No consideration given to mitiga- tion measures for ecological con- tinuum. * No consideration given to mitiga- tion measures for ecological con- tinuum.

11. Setting of reference conditions Reference criteria. Reference criteria. * Only minor differences among countries. * Only minor differences among countries. * Land use %, based on Corine Land Cover analysis. “Natural” > 70 %. * Land use %, based on Corine Land Cover analysis. “Natural” > 70 %. * None of the reservoirs (except one) are located downstream from an upper dam. * None of the reservoirs (except one) are located downstream from an upper dam. * Additionally: * Additionally: a) Upstream accumulated water demand for different uses (ES); b) overall impact index (FR); c) evidence of very low pressures a) Upstream accumulated water demand for different uses (ES); b) overall impact index (FR); c) evidence of very low pressures

12. REFERENCE RESERVOIR DATASET RESERVOIR NAME COUNTRYTYPE Chlorophyll-a (mg/m 3 ) Total Biovol. (mm 3 /l) % Cyanobacteria Biovolume Catalan Index TEHNITI LIMNITR OPOUGR Siliceous “Wet”1,100,360,000,11 VILARINHO AS FURNASPT Siliceous “Wet” 0,740,071,170,76 CASTELO DE BODE*PT Siliceous “Wet” 1,370,270,000,02 SALIMEES Siliceous “Wet” 3,730,690,000,63 LA RIBEIRAES Siliceous “Wet” 2,612,280,000,11 LEFKARACYCalcareous0,380,560,13 SAINT CASSIENFR Calcareous 2,53--- SACELEROCalcareous0,540,810,000,02 ARENÓSESCalcareous1,920,840,001,09 EUGUIESCalcareous1,810,650,001,41 * Best available

13. Setting of reference conditions Procedure Procedure * For each ref. site and index, summer average values from the 3-4 sampling dates in June-Sept. 2005 * For each ref. site and index, summer average values from the 3-4 sampling dates in June-Sept. 2005 * Summary statistics: median of the summer average values, within each type, for every biological index. * Summary statistics: median of the summer average values, within each type, for every biological index.

14. Setting of reference conditions Type-specific reference values Type-specific reference values Type Chlorophyll-a (mg/m 3 ) Total Biovolume (mm 3 /l) % Cyanobacteria Biovolume Catalan Index Silic. “Dry” Not sufficient sites. To be set by expert judgement in the next IC stage. Not sufficient sites. To be set by expert judgement in the next IC stage Silic. “Wet” 1,40,3600,1 Calca- reous 1,80,7600,61

15. Setting of Boundaries For High/Good boundary For High/Good boundary * According to the WFD, it is not required to report HMWB’s at Maximum Ecological Potential, but only those being at “good and above” Ecological Potential. * According to the WFD, it is not required to report HMWB’s at Maximum Ecological Potential, but only those being at “good and above” Ecological Potential. * Therefore, intercalibrating for Mx/G boundary makes no sense (unless performed as an instrument for G/M boundary setting). * Therefore, intercalibrating for Mx/G boundary makes no sense (unless performed as an instrument for G/M boundary setting).

16. Setting of Boundaries For Good/Moderate boundary For Good/Moderate boundary * G/M boundary setting was based on the data especially collected from all the GIG sites in the IC network draft register. In accordance with WFD Annex V, these sites were selected by expert judgement and all available information. * No need nor possibility to follow thoroughly the BSP manual. * G/M boundaries were set as the summary statistics shown below, each site being represented by its summer mean value: Biomass metrics…………………..95 percentile Biomass metrics…………………..95 percentile Composition metrics……………..90 percentile Composition metrics……………..90 percentile

17. Setting of Boundaries Compliance with WFD normative definitions Compliance with WFD normative definitions Step 6 BSP: Analysis of paired metrics to locate class boundaries. Step 6 BSP: Analysis of paired metrics to locate class boundaries. Analysis performed on the summer 2005 LM GIG data, merged to a set of previous data from an array of Spanish reservoirs (C. de Hoyos, 2005 ) Analysis performed on the summer 2005 LM GIG data, merged to a set of previous data from an array of Spanish reservoirs (C. de Hoyos, 2005 )

18. Chloropyll a Cyanobacteria and Chrysophyte Data of the intercalibration exercise 7.9 Cyanobacteria and non colonial Diatoms Data of the intercalibration exercise (summer 2005) + Spanish reservoirs (1999-2001) (C. de Hoyos, 2005)

19. FINAL OUTCOME OF THE INTERCALIBRATION G/M BOUNDARY VALUES TYPE Chlorophyll-a G/M (mg/m 3 ) Total Biovol. G/M (mm 3 /l) % Ciano. Biov. G/M Catalan Index G/M Siliceous “Dry”8,31,646,315,9 Siliceous “Wet”6,71,99,210,6 Calcareous4,22,128,57,73

20. Setting of Boundaries Procedure for EQR assessment Procedure for EQR assessment * The same approach as in the L-A GIG. * Logarithmic functions to convert the metrics class boundary values into a EQR scale with equal class range widths: EQR=-a L n I+b EQR=-a L n I+b * Mx/G boundary values (EQR=0.8) estimated as the 75 percentile of the reference values.

22. Transformation of the IC results into the national typologies/assessment systems Transformation of the IC results into the national typologies/assessment systems No specific information was collected about national plans or steps, but informal contacts have been started within each country. No specific information was collected about national plans or steps, but informal contacts have been started within each country.

23. Overall advantages Comparability and consistency, fully garanteed from the onset. Comparability and consistency, fully garanteed from the onset. The agreed sampling and lab methods, clearly more suitable for comparability and reliability, given the demands of the WFD Annex V. The agreed sampling and lab methods, clearly more suitable for comparability and reliability, given the demands of the WFD Annex V. No need to assess the ref. cond. prior to G/M boundary setting in terms of the “observed” values of the biological parameters. “Deviations” are implicitedly accounted for in a proper selection of IC sites. No need to assess the ref. cond. prior to G/M boundary setting in terms of the “observed” values of the biological parameters. “Deviations” are implicitedly accounted for in a proper selection of IC sites.

24. Main problems, gaps, difficulties encountered in the process of IC Scarcity of suitable biological data for IC purposes. Scarcity of suitable biological data for IC purposes. Need to review the assessment of type- specific ref. cond. and the criteria for selection of IC network sites. Need to review the assessment of type- specific ref. cond. and the criteria for selection of IC network sites. Neet to split the Calcareous type into “wet” and “dry”. Neet to split the Calcareous type into “wet” and “dry”. Difficulties may be found by some countries in enforcing the IC results and consequently adapting their national typologies and classification systems. Difficulties may be found by some countries in enforcing the IC results and consequently adapting their national typologies and classification systems.

25. CONTINUATION OF THE IC PROCESS It is important to continue the IC process in order to expand its scope to other biological quality elements. It is important to continue the IC process in order to expand its scope to other biological quality elements. But not less important is to continue it for improving the current results But not less important is to continue it for improving the current results