1. Genetic monitoring methodologies for allogamous crop wild relatives Group B - results

2. Sampling Annual – every year Perennials – as often (generation time)

3. Risk of loss of diversity Measuring loss of diversity –Morphological characters evolution –Molecular tools for evolution

4. Loss genetic diversity within population decreasing viability extinction Losing interesting variation within populations

5. For the study of evolution in genetic drift, need to use genetic diversity indexes (indicators of overall diversity) Or To use tools for specific useful traits to monitor over time

6. 3 kinds of markers levels 1.Diversity 2.Gene marker or processes 3.Fitness (related to specific traits) 1.Demographic studies 2.Related to fecundity Differentiate between genetic and environmental stochasticity

7. Knowledge of heritability molecular characters – use it as surrogate of genetic diversity Use list of descriptors as a base for cwr characterisation

8. Ex situ / in situ –Link –Coordination –Ex situ bottleneck in characterisation/evaluation, useful for in situ

9. Spatial structure (key point) –Distribution of individuals (further inbreeding) –Spatial structure, diversity on morphological characters –Genetic neighbourhood –Implication in restoration

10. Number of sample/periodicity –Higher and more frequent than for autogamous How to sample –Allogamous – Random / stratified –Vegetatively propagated – –If knowledge available – random –If knowledge is not available – random / stratified

11. RECOMMENDATIONS Genetic reserve should have a backup ex situ collection In situ/ex situ characterisation –To promote use Carry out in depth studies on cwr –Useful information on genetic knowledge

12. Reinforce links with ex situ conservation –Documentation –Improve ecogeographic survey Development of primers –Sources Genebanks Breeding institutes Universities CG centres At the National level – set priorities for developing primers

13. Transferability of primers are not easy Policy issues, germplasm exchange required –Take into consideration any international agreements, conventions, treaties,…