1. Aquatic Invertebrates in Pool and Riffle Habitats of Blackburn Fork Michael Railling Wildlife and Fisheries Science Tennessee Tech

2. Project summary Problem: Are the aquatic invertebrates the same in pool and riffle habitats. Problem: Are the aquatic invertebrates the same in pool and riffle habitats. Objective: Show that the two habitats should be comparably different by scoring the different taxa. Objective: Show that the two habitats should be comparably different by scoring the different taxa. Expected Results: In the pool more tolerant taxa will be found. The Riffle will support taxa that are more susceptible to poor water quality. Expected Results: In the pool more tolerant taxa will be found. The Riffle will support taxa that are more susceptible to poor water quality.

3. Introduction/Background Stream macroinvertebrate assemblage varies at both regional and local scales (Heino et al. 2002). My experiment the samples are taken only feet away from each other, however the Taxa Richness and Percent EPT’s should be different. Stream macroinvertebrate assemblage varies at both regional and local scales (Heino et al. 2002). My experiment the samples are taken only feet away from each other, however the Taxa Richness and Percent EPT’s should be different. Lower water quality is directly associated to diversity (Heino et al. 2002). The water quality of pool areas is lower than that of Riffles; causing less diversity. Lower water quality is directly associated to diversity (Heino et al. 2002). The water quality of pool areas is lower than that of Riffles; causing less diversity.

4. Objective/Hypothesis Statement Hypothesis: Hypothesis: What different proportions of Genera can be found in pool and riffle habitats of the Blackburn Fork, and can the differences of genera be linked to different water quality within theses habitatats. Objective: Objective: To show that different genera are found in different habitats. Using Taxa Richness and Percent EPT’s

5. Methods Using 6 Hester-Dendy samplers Qualitatively, 3 in pools and 3 in riffles. Using 6 Hester-Dendy samplers Qualitatively, 3 in pools and 3 in riffles. Scrubbing the bugs off the substrate Scrubbing the bugs off the substrate Identifying the invertebrates down to genus. Identifying the invertebrates down to genus. Scoring the results using data forms to show the Taxa Richness and Percent EPT’s (State of Tennessee 2002). Scoring the results using data forms to show the Taxa Richness and Percent EPT’s (State of Tennessee 2002).

6. Materials Six Hester-Dendy Samplers with nine three inch plates on each. Six Hester-Dendy Samplers with nine three inch plates on each. One pint Formalin One pint Formalin One pint Ethyl alcohol One pint Ethyl alcohol One D-Frame net One D-Frame net 12 bricks 12 bricks One case of viles One case of viles One large cooler One large cooler 24 freezer bags 24 freezer bags

7. Expected Results and Benefits Expected Results: In the pool more tolerant taxa will be found. The Riffle will support taxa that are more susceptible to poor water quality such as EPT’s. Expected Results: In the pool more tolerant taxa will be found. The Riffle will support taxa that are more susceptible to poor water quality such as EPT’s. Benefits: Showing that invertebrates are habitat specific. Benefits: Showing that invertebrates are habitat specific.

8. Project Timeline Title: 8/20/03-9/03/03 Title: 8/20/03-9/03/03 Objective: 9/10/03-9/17/03 Objective: 9/10/03-9/17/03 Literature References: 9/17/03-present Literature References: 9/17/03-present Data Form: 9/24/03-present Data Form: 9/24/03-present Experiment: 8/9/03-present Experiment: 8/9/03-present

9. Literature Cited Adams, S.M., W.R. Hill, M.J. Peterson, M. G. Ryon, J. G. Smith, and A.J. Stewart. 2002. Assessing recovery in a stream ecosystem: Applying chemical and biological endpoints. Ecological Applications 12: 1510-1527. Adams, S.M., W.R. Hill, M.J. Peterson, M. G. Ryon, J. G. Smith, and A.J. Stewart. 2002. Assessing recovery in a stream ecosystem: Applying chemical and biological endpoints. Ecological Applications 12: 1510-1527. Battin, T.J., L.A. Kaplan, J.D. Newbold, and Susan Hendricks. 2003. A mixing model of stream solute dynamics and the contribution of a hyporheic zone to ecosystem function. Freshwater Biology 48: 995- 1014. Battin, T.J., L.A. Kaplan, J.D. Newbold, and Susan Hendricks. 2003. A mixing model of stream solute dynamics and the contribution of a hyporheic zone to ecosystem function. Freshwater Biology 48: 995- 1014. Carter, J.L., and V.H. Resh. 2001. After site selection and before data analysis: Sampling, sorting, and labratory procedures used in stream benthic macroinvertebrates monitoring programs by USA state agencies. Journal of the North American Benthological Society 20: 658-682. Carter, J.L., and V.H. Resh. 2001. After site selection and before data analysis: Sampling, sorting, and labratory procedures used in stream benthic macroinvertebrates monitoring programs by USA state agencies. Journal of the North American Benthological Society 20: 658-682. Dyer, S.D. and Xinhao Wang. 2002 A comparison of stream biological responses to discharge from wastewater treatment plants in high and low population density areas. Environmental Toxicology and Chemistry 21: 1065-1075. Dyer, S.D. and Xinhao Wang. 2002 A comparison of stream biological responses to discharge from wastewater treatment plants in high and low population density areas. Environmental Toxicology and Chemistry 21: 1065-1075. Fairchild, M.P., and Joseph P. Holomuzki. 2002. Spatial variability and assemblage structure of stream hydrosychid caddisflies. Journal of the North American Benthological Society 21: 576-590. Fairchild, M.P., and Joseph P. Holomuzki. 2002. Spatial variability and assemblage structure of stream hydrosychid caddisflies. Journal of the North American Benthological Society 21: 576-590. Heino, J., T. Muotka, and R. Paavola. 2003. Determinates of macroinvertebrate diversity in headwater streams: Regional and local influences. 2003. Journal of Animal Ecology 72: 425-434. Heino, J., T. Muotka, and R. Paavola. 2003. Determinates of macroinvertebrate diversity in headwater streams: Regional and local influences. 2003. Journal of Animal Ecology 72: 425-434. Hoffman, A., and V.W. Resh. Oviposition in three species of limnephiloid caddisflies(Trichoptera): Hierarchical influences on site selection. 2003. Freshwater Biology 48:1064-1077. Hoffman, A., and V.W. Resh. Oviposition in three species of limnephiloid caddisflies(Trichoptera): Hierarchical influences on site selection. 2003. Freshwater Biology 48:1064-1077. Hyne, R.V., and W.A. Maher. Invertebrate biomarkers: Links to toxicosis that predict population decline. 2003. Ecotoxicology and Environmental Safety 54:366-374. Hyne, R.V., and W.A. Maher. Invertebrate biomarkers: Links to toxicosis that predict population decline. 2003. Ecotoxicology and Environmental Safety 54:366-374. Kobayashi, S., and T. Kagaya. 2002. Differences in litter characteristics and macroinvertebrate assemblages between litter patches in pools and riffles in headwater stream. Limnology 3:37-42. Kobayashi, S., and T. Kagaya. 2002. Differences in litter characteristics and macroinvertebrate assemblages between litter patches in pools and riffles in headwater stream. Limnology 3:37-42. Malmqvist, B. 2002. Aquatic invertebrates in riverine landscapes. Freshwater Biology 47:679-694. Malmqvist, B. 2002. Aquatic invertebrates in riverine landscapes. Freshwater Biology 47:679-694. Negishi, J.N., M. Inoue, and M. Nunokawa. 2002. Effects of channelisation on stream habitat in relation to a spate and flow refugia for macroinvertebrates in northern Japan. 2002. Freshwater Biology 47:1515-1529. Negishi, J.N., M. Inoue, and M. Nunokawa. 2002. Effects of channelisation on stream habitat in relation to a spate and flow refugia for macroinvertebrates in northern Japan. 2002. Freshwater Biology 47:1515-1529. Parsons, M., M.C. Thoms, and R.H. Norris. 2003. Scales of macroinvertebrate distribution in relation to the hierarchical organization of river systems. Journal of the Americam Benthological Society 22: 105-122. Parsons, M., M.C. Thoms, and R.H. Norris. 2003. Scales of macroinvertebrate distribution in relation to the hierarchical organization of river systems. Journal of the Americam Benthological Society 22: 105-122. State of Tennessee. 2002. Quality System Standard Operating Procedure for Macroinvertebrate Stream Surveys. Department of Environment and Conservation. State of Tennessee. 2002. Quality System Standard Operating Procedure for Macroinvertebrate Stream Surveys. Department of Environment and Conservation. Wymer, W.A., and S.B. Cook. 2003. Effects of Chironomidae (Diptera) taxanomic resolution on multivariate analysis of aquatic insect communities. Journal of Freshwater Ecology 18:179-186. Wymer, W.A., and S.B. Cook. 2003. Effects of Chironomidae (Diptera) taxanomic resolution on multivariate analysis of aquatic insect communities. Journal of Freshwater Ecology 18:179-186.

10. Budget Saleries and Wages Year 1 Year 2 Professional $150.00 $150.00 Technician $65.00 $65.00 Secretarial $90.00 $90.00 Subtotal $305.00 $305.00 Benefits $ - $ - Travel $25.00 $25.00 Non- Expendables $100.00 $100.00 Expendables Computer $700.00 $ - Supplies and Expenses $278.13 $6.94 Copying and Telephone $20.00 $20.00 Subtotal $998.13 $26.94 Indirect Cost $125.00 $125.00 Direct Cost $1303.13 $331.94 Total Cost $1428.13 $456.94 = 1885.17