1. Dasatinib or Imatinib (IM) in Newly Diagnosed Chronic Myeloid Leukemia in Chronic Phase (CML-CP): Two-Year Follow-Up from DASISION Kantarjian H et al. Proc ASCO 2011;Abstract 6510.

2. Dasatinib 100 mg QD (n = 259) Imatinib 400 mg QD (n = 260) Eligibility Philadelphia chromosome- positive CML-CP within 3 months from diagnosis No prior therapy, excluding anagrelide or hydroxyurea Stratification Hasford risk score R Kantarjian H et al. Proc ASCO 2011;Abstract 6510. Primary Endpoint: Confirmed CCyR by 12 months Secondary/Other Endpoints: Rates of CCyR and MMR; times to confirmed CCyR and MMR; time in confirmed CCyR; PFS; OS Follow-up 5 yrs DASISION Study Design

3. 73 85 86 59 73 79 82 0 10 20 30 40 50 60 70 80 90 100 DASISION: Cumulative CCyR Rates by Months of Treatment (ITT Population) Kantarjian H et al. Proc ASCO 2011;Abstract 6510. 6121824 Months CCyR (%) Dasatinib 100 mg QD Imatinib 400 mg QD

4. DASISION: Cumulative Incidence of MMR With permission from Kantarjian H et al. Proc ASCO 2011;Abstract 6510. By 12 months 46% 28% By 24 months 64% 46% p < 0.0001 Months MMR (%) Dasatinib 100 mg QD Imatinib 400 mg QD

5. DASISION: Transformation to AP/BP CML (ITT Population) Kantarjian H et al. Proc ASCO 2011;Abstract 6510. a Yearly evaluations after discontinuation are currently stipulated by the protocol; additional information on patient status may be provided by the investigators at other times. Including follow-up beyond discontinuation a Dasatinib 100 mg QD Imatinib 400 mg QD On study Patients (%) 6/259 13/2609/259 15/260 100 6 4 2 0 2.3 5.0 3.5 5.8

6. DASISION: Difference in Adverse Event Rates for Dasatinib and Imatinib With permission from Kantarjian H et al. Proc ASCO 2011;Abstract 6510. Patients w/QTc intervals 450 msec - 500 msec: Dasatinib 2%, Imatinib 4% Median QTc interval change from baseline: Dasatinib 3.0 msec, Imatinib 8.2 msec Fluid retention Superficial edema Pleural effusion Myalgia Nausea Vomiting Diarrhea Fatigue Headache Rash Neutropenia Thrombocytopenia Anemia Any grade Grade 3/4 Rate difference (dasatinib–imatinib) with exact 95% CI Favors dasatinibFavors imatinib -40 -20 0 20 40

7. Kantarjian H et al. Proc ASCO 2011;Abstract 6510. Conclusions 24-month follow-up of patients with newly diagnosed CML- CP in the DASISION trial continues to demonstrate –High rate of CCyR with dasatinib –Higher and faster rate of MMR with dasatinib over imatinib Few patients transformed to AP/BP CML –6 on dasatinib, 13 on imatinib Dasatinib was associated with fewer discontinuations due to toxicity (data not shown) –Frequency of many of the most common nonhematologic AEs were comparable or lower than imatinib –Most cytopenias occurred within the first year Longer follow-up continues to support the use of dasatinib 100 mg once daily as first-line treatment of newly diagnosed CML-CP

8. Investigator Commentary: DASISION Study of Dasatinib versus Imatinib in Newly Diagnosed CML-CP The primary endpoint of DASISION was confirmed complete cytogenetic response (CCyR) at 12 months. Now with 18-month follow-up, the response rates were clearly higher for dasatinib compared to imatinib. So the relevance of this follow-up is that this is an endpoint at 18 months that would clearly define failure of imatinib if CCyR is not reached. With this clinically relevant endpoint, the second-generation TKI dasatinib is clearly better than imatinib as up-front therapy for CML-CP. Generally, I will use a second-generation TKI up front, with my selection based on comorbidities. If I have patient who has congestive heart failure who I know is prone to pleural effusions, I might not choose dasatinib. If I have a diabetic patient, who can’t eat around the time that he or she receives nilotinib, I might not choose nilotinib for that patient. I don’t believe one can say at this point that if you want to use a second- generation TKI you can choose on the basis of efficacy. We have nothing to suggest, at this point, that one is better than the other. The toxicity profiles are slightly different, and nilotinib is administered BID, whereas dasatinib has the advantage of being once a day. Susan M O'Brien, MD