Elena Klenova CTCF and BORIS in normal development, epigenetics and tumourigenesis Areas of research: Molecular Oncology Gene regulation Translational.

Slides:

Advertisements

Similar presentations

Characterization of Transcriptional Regulatory Networks controlling plant cell adaptation to environmental stresses.

Advertisements

CTCF: the protein, the binding partners, the binding sites and their chromatin loops by Sjoerd Johannes Bastiaan Holwerda, and Wouter de Laat Philosophical.

Proteomics Examination Yvonne (Bonnie) Eyler Technology Center 1600 Art Unit 1646 (703)

Data integration across omics landscapes Bing Zhang, Ph.D. Department of Biomedical Informatics Vanderbilt University School of Medicine

Prostate cancer: early detection, monitoring and prognosis: Essex Biomedical Sciences Institute (EBSI) Elena Klenova, University of Essex the search for.

Gene regulation in cancer 11/14/07. Overview The hallmark of cancer is uncontrolled cell proliferation. Oncogenes code for proteins that help to regulate.

IDENTIFICATION OF THE MOLECULAR MECHANISMS IN RETT SYNDROME AND RELATED DISORDERS (RTT-GENET) X.

E2A and acute lymphoblastic leukemias (ALL). A closer look at the E2A gene... Other names: TCF3, ITF1, and Factors E12/E47 Located on chromosome 19 Encodes.

Epigenetics 12/05/07 Statisticians like data.

Introduction to Genomics, Bioinformatics & Proteomics Brian Rybarczyk, PhD PMABS Department of Biology University of North Carolina Chapel Hill.

CISC667, F05, Lec24, Liao1 CISC 667 Intro to Bioinformatics (Fall 2005) DNA Microarray, 2d gel, MSMS, yeast 2-hybrid.

Estrogen and its receptors play an important role in breast carcinogenesis. In humans, there are two subtypes of estrogen receptors (ER), ER  and ER ,

 MicroRNAs (miRNAs) are a class of small RNA molecules, about ~21 nucleotide (nt) long.  MicroRNA are small non coding RNAs (ncRNAs) that regulate.

More regulating gene expression. Fig 16.1 Gene Expression is controlled at all of these steps: DNA packaging Transcription RNA processing and transport.

When mammalian cells are subjected to stress signals, oxygen deficiency, radiation, DNA damage, or Chemo- therapeutic drugs, p53 is activated, leading.

Computational Molecular Biology Biochem 218 – BioMedical Informatics Gene Regulatory.

Epigenetic modification of DNA

Epigenome 1. 2 Background: GWAS Genome-Wide Association Studies 3.

CEITEC BRNO | CZECH REPUBLIC central european institute of technology CEITEC Genomics and proteomics at MU Jiří Fajkus.

The virochip (UCSF) is a spotted microarray. Hybridization of a clinical RNA (cDNA) sample can identify specific viral expression.

Epigenetics of Human Marie Černá Lecture No 426-H.

Finish up array applications Move on to proteomics Protein microarrays.

BIOMARKERS Diagnostics and Prognostics. OMICS Molecular Diagnostics: Promises and Possibilities, p. 12 and 26.

Doug Brutlag 2011 Genomics, Bioinformatics & Medicine Doug Brutlag Professor Emeritus of.

分子病理實驗室 Molecular Pathology Lab 實驗室主持人：戚謹文, 周德盈共同主持人 : 李芬瑤.

SmallRNAs Small molecules, big functions. Brief history The first described microRNA, lin-4 was cloned and characterised as a translational repressor.

Integrating the Bioinformatic Technology Group into your research programme Introduction People and Skills Examples Integrating the BTG Contacts BHRC Away.

Copyright © 2009 Pearson Education, Inc. Regulation of Gene Expression in Eukaryotes Chapter 17 Lecture Concepts of Genetics Tenth Edition.

Michael Cummings David Reisman University of South Carolina Gene Regulation Part 2 Chapter 9.

COMPUTATIONAL ANALYSIS OF MULTILEVEL OMICS DATA FOR THE ELUCIDATION OF MOLECULAR MECHANISMS OF CANCER Presented by Azeez Ayomide Fatai Supervisor: Junaid.

Transcriptional - These mechanisms prevent transcription. Posttranscriptional - These mechanisms control or regulate mRNA after it has been produced.

Bioinformatics MEDC601 Lecture by Brad Windle Ph# Office: Massey Cancer Center, Goodwin Labs Room 319 Web site for lecture:

Proteomics Session 1 Introduction. Some basic concepts in biology and biochemistry.

OMICS International welcomes submissions that are original and technically so as to serve both the developing world and developed countries in the best.

Decoding the Network Footprint of Diseases With increasing availability of data, there is significant activity directed towards correlating genomic, proteomic,

Central dogma: the story of life RNA DNA Protein.

TOXICOGENOMICS.

Histone Methyltransferases: Global Industry Report for Research Tools, Diagnostics and Drug Discovery

Greg Brooke Molecular Oncology. Prostate Cancer - Leading Cause of Cancer- related death in men - Growth dependent on androgens - Therapy targets androgen.

Lecture 11. Topics in Omic Studies (Cancer Genomics, Transcriptomics and Epignomics) The Chinese University of Hong Kong CSCI5050 Bioinformatics and Computational.

Triplex forming oligonucleotides (TFO)

Trends Biomedical Science

Research questions Regulation of eukaryotic signal transduction: focussing on protein phosphorylation and protein-protein interactions (in the context.

Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker Yun-Ji Kim 1 *, Jae-Won Huh 1,2 *, Dae-Soo Kim 3, Min-In Bae 1,

Outline Molecular Cell Biology Assessment Review from last lecture Role of nucleoporins in transcription Activators and Repressors Epigenetic mechanisms.

Cancer Cancer- a malignant tumor; the result of abnormal cell proliferation. Regulation of Cell Division –Tumor Supressor Genes Genes that inhibit cell.

Chapter 2. Amino acids Protein structure Primary:

Homework #2 is due 10/17 Bonus #1 is due 10/24 Office hours: M 10/ :30am 2-5pm in Bio 6.

Current Biological Approaches for Radiation Research Aleksandra Ristić Fira University of Belgrade - Vinča Institute of Nuclear Sciences, Belgrade, Serbia.

Bmi-1 in Cancer Cancer genetics 2012/04/ 전종철

Regulation of transcription in eukaryotes

Different microarray applications Rita Holdhus Introduction to microarrays September 2010 microarray.no Aim of lecture: To get some basic knowledge about.

Novel blood and tissue Biomarkers for Breast and Prostate Cancers

High-throughput data used in bioinformatics

GENETIC BIOMARKERS.

Estrogen & Estrogen Receptor

Interrogation of cross talk between proteins and gene regulatory networks in breast cancer Chambers, Teressa Lee Hiren Karathia Sridhar Hannenhalli.

Bioinformatic Tools for Epigenetic Research

Epigenetic modulators, modifiers and mediators

Relationship between Genotype and Phenotype

“Proteomics is a science that focuses on the study of proteins: their roles, their structures, their localization, their interactions, and other factors.”

Volume 56, Issue 2, Pages (August 2009)

Proteomics Informatics David Fenyő

Different Genes ~ Protein Primary Structure

pRB AND CELLULAR SENESCENCE

Epigenetics modification

Galaxy course EMC TraIT Nov 2014_Jenster

Relationship between Genotype and Phenotype

Epigenetic regulation of p16INK4a in human gastric cancer.

Tenets of PTEN Tumor Suppression

Presentation transcript:

Elena Klenova CTCF and BORIS in normal development, epigenetics and tumourigenesis Areas of research: Molecular Oncology Gene regulation Translational research : clinical links via EBSI

CTCF and BORIS CTCF, named for “CCTC binding factor” in 1990 BORIS, for “Brother of the Regulator of Imprinted Sites” in 2002 Transcription factors with common DNA – binding domain: Normally expressed in testis Ubiquitously expressed

CTCF and its known modifications and interacting partners Ohlsson et al, 2010

CTCF plays an important role in the regulation of nuclear architecture: Genome-wide CTCF-mediated chromatin interactome Handoko et al., 2011

Theme 1. Role of CTCF and BORIS in normal development and tumourigenesis. Cellular functions regulated by BORIS and CTCF ( apoptosis, proliferation); role of post-translational modifications and protein partners. Individual genes regulated by CTCF and BORIS. Genome-wide studies of gene expression and CTCF/BORIS binding to DNA. Mechanisms of BORIS abnormal activation and cancer. Tumour immunology (interaction between tumours and immune cells). Structural analysis of CTCF/DNA complexes. CTCF and BORIS in stem cells (potential). CTCF and viral infection (potential). Collaborations: Existing - Metodi, Nelson, Philippe, Igor Potential – Greg, Ralf/Andrea, Glyn, Mike H.

Models: cell lines, primary cells and tumour tissue specimens. Methods: Manipulation with gene expression levels ( siRNA, ectopic expression, stable and inducible cell lines, lentiviruses ( in progress); DNA manipulations (mutagenesis, methylation etc); Imaging ( immunocytochemistry, immunohistochemistry, immunofluorescene staining); Proteomics; Genomics (RNA-Seq, ChIP-Seq, microarray); Bioinformatics (data analysis and interpretation); Biological tests: proliferation (MTT), apoptosis (TUNEL). Theme 1. The role of CTCF and BORIS in tumourigenesis: models and methods

Theme 2. Novel cancer biomarkers and drug targets Identification and characterization of novel cancer biomarkers (tissue, blood and potentially urine) and drug targets (tissue). Assessment of CTCF and BORIS as potential diagnostic and prognostic biomarkers in common cancers, such as breast and prostate tumours. Collaborations: Existing: Metodi, Harry. Potential: Phil R, Chris R, Berthold.

Theme 2. Novel cancer biomarkers and drug targets. Materials and methods Materials: Blood, tissues; urine ( in progress) Methods: Proteomics ( 2xD gel electrophoresis, mass-spectrometry); Genomics + bioinformatics ( mainly existing databases); Statistics