Maryakhina V.S., Gun’kov V.V. Orenburg state university, Russia Saratov fall meeting- 2015

Exemplary fluorescence images of one animal after injection 2 μ mol/kg TSC (tetrasulfonated carbocyanine) intravenously into F9-teratocarcinoma bearing mice Christin Perlitz, et all //Journal of Fluorescence, Vol. 15, No. 3, 2005 Saratov fall meeting- 2015

Enzyme Cells Photosensitizer monomer Enzyme dimer Photosensitizer monomer fixed moving Saratov fall meeting- 2015

Maryakhina V.S., Gunkov V.V. Computer research and modeling, 2013 Dynamics of bengal rose fluorescence in model solution of collagenase with mammary cells Saratov fall meeting- 2015

Concentration changes of photosensitizer molecules at immobilization process received from two models Saratov fall meeting- 2015

Maryakhina V.S., Letuta S.N.// Laser physics V p eosine bengal rose М М bengal roseeosine Saratov fall meeting- 2015

Comparison of experimental and theoretical results The normalized experimental (1,2) and theoretical (3,4) fluorescence spectra of erythrosine in collagenase solution 0.1 mkg/ml after addition of dye aliquot with initial concentration: 1, m М ; 2,4- 1 m М. Maryakhina V.S., Gun’kov V.V. Computer research and modeling V.5.- №5.- p Saratov fall meeting- 2015

Fluorescence intensivity dynamics at 560 nm Enzyme 560 nm 545 nm C enzyme = 0.5 mg/ml Maryakhina V.S., Gun’kov V.V. Computer research and modeling V.5.- №5.- p C enzyme = 0.1 mg/ml 0.5 mg/ml Saratov fall meeting- 2015

For biological liquid flow (blood, for example) - differential equation of convective diffusion (moving medium) a few phases w w, w p, w cell - rate of water, peptide and cell phases assumptions - w w = w p = w cell - movable coordinates x=r+wt Maryakhina V.S., Gun’kov V.V.// JIOHS, 2016, V.9 (4) Saratov fall meeting- 2015

Initial and bordering conditions d vessel = 0.5 cm R i - average peptides / cells size, nm; R- size of the injected compound molecule, nm. 0.2 cPz0.4 cPz0.6 cPz0.8 cPz1 cPz D p ·10 -12, m 2 /s D cell ·10 -15, m 2 /s D w ·10 -9, m 2 /s cPz0.4 cPz0.6 cPz0.8 cPz1 cPz k 1 ·10 -20, m 3 /s k 2 ·10 -17, m 3 /s plasma is 60 % from blood volume; different cell types are the rest 40 % while peptide molecules are 8 % in plasma. Saratov fall meeting- 2015

The kinetic curves of the injected compound concentrations in water, cells and peptide phases for different cell concentrations C cell (from 30 % to 50 % ) Maryakhina V.S., Gun’kov V.V.// Journal of innovative optical health sciences, 2016, V.9 (4) Saratov fall meeting- 2015

Dynamics of fluorescence spectra change of bengal rose. λex= 515 nm. C cell (x,0)=0.4 The experimental fluorescence spectra of bengal rose in: 1- buffer solution, 2- in blood serum albumin, 3- cancer mammary cells suspension. Saratov fall meeting- 2015

Changes of bengal rose fluorescence spectrum intensity at 560, 590 and 580 nm to time for different cell concentrations Saratov fall meeting- 2015

The experimental setup for investigation of distribution of the injected compound in blood flow - pump - pressure sensor This experimental setup was created in our laboratory. It is a system of polymer tubes in which model liquid circulate. The first experiments will be soon. Saratov fall meeting- 2015

This work is partially supported by grant of RFBR № mol_a and president fellowship № SP for young scientists