Regulation of the eukaryotic cell cycle. Chapter 25 (3rth edition) Chapter 13 (4rth edition)

24 Hrs Cell Cycle: G1 = 9 HrsS phase = 10 Hrs G2 = 4.5 Hrsmitosis = 0.5 Hrs.

(APC Specificity Factor)

Cdc2 - Cdc2 D WT

Kinase phosphorylates inhibitor y tyrosine (Y15) and Threonine (T14) Phosphatase removes inhibitory phosphates from Y15 and T14

Interphase Prophase Metaphase Anaphase Telophase

Chromosomal Passenger Complex

Red: Tubulin; Blue: DNA; Green: Aurora B (CPC); Magenta: Centromere. Bi-oriented chromosome under tension at metaphase.

Cdc20 Cdh1

Clb = Mitotic Cyclins Cln = G1 and G1/S Cyclins

Cells with different bud stages CDK deficiency: Cells w/o buds Separation deficiency: Cells with large bud

Cln3-CDK G1 cyclin - CDK Whi5 SBF/MBF Whi5-P SBF/MBF Cln1/Cln2 Clb5/Clb6 S-phase cyclin – CDK Sic1 Clb6/Clb6 + Sic1-P SCF Phosphorylation pre- replication complexes Cdh1 Cdh1-P DNA polymerase DNA ligase dNTP reductase Sic1-P Poly-ubiquitylation

Clb = Mitotic Cyclins Cln = G1 and G1/S Cyclins

DND stainBrDU stainAnti-cyclin D stain

Entry into the growth cycle by binding of a growth factor to a growth factor receptor

(Transcription Factors)

Lee Hartwell et al..

( end S phase) (end metaphase) (end anaphase)

Four checkpoints: 1.Growth Checkpoint. 2.DNA-damage Checkpoints 3.Spindle Assembly Checkpoints 4.Spindle position Checkp[oints.

Cell Cycle Yeast

Linking cell growth to initiation of cell cycle in S. pombe.

Spindle assembly checkpoint