Simple Reporting of Complex DNA Evidence: Automated Computer Interpretation Promega 14th International Symposium on Human Identification Pointe Hilton.

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Presentation transcript:

Simple Reporting of Complex DNA Evidence: Automated Computer Interpretation Promega 14th International Symposium on Human Identification Pointe Hilton Tapatio Cliffs Resort Phoenix, AZ October, 2003

Single source: Peak  allele. At locus, report the genotype.

Data Issues: Peak height/area Peak size PCR stutter Pref amp Mixed samples Low amounts Degraded DNA Many samples Many contributors Contributor count Background noise Random variation LCN PCR

Math/stat models: Peak height/area Peak size PCR stutter Pref amp Mixed samples Low amounts Degraded DNA Many samples Many contributors Contributor count Background noise Random variation LCN PCR Single source: Peak  allele. At locus, for contributor, report the genotype(s).

Recent Revolutions science science PCR, sequencer technology technologycomputer law lawDaubert statistics statistics computational truth

TrueAllele™ Expert system a computer program that replicates human expertise History System: 2  3 Version: 14  15

Admissibility Rule Data 2.Method 3.Application Daubert 1.Testable 2.Error rate 3.Peer review 4.General acceptance

Validation Design 10%30%50%70%90% 1, 1/2, 1/4, 1/8 ng

STR Data Generation premixed DNA templates: NIST premixed DNA templates: NIST lab protocols: Cybergenetics lab protocols: Cybergenetics data generation (ten DNA labs) data generation (ten DNA labs) Florida, New York, Ohio, Pennsylvania, Florida, New York, Ohio, Pennsylvania, Virginia, Cellmark, UK FSS, Cybergenetics Virginia, Cellmark, UK FSS, Cybergenetics DNA sequencers: DNA sequencers: FMBio/II, 377, 310, 3100, 3700 FMBio/II, 377, 310, 3100, 3700 STR panels: PowerPlex (1, 2, 16), STR panels: PowerPlex (1, 2, 16), ProfilerPlus, Cofiler, SGMplus, Identifiler ProfilerPlus, Cofiler, SGMplus, Identifiler

Data Analysis TrueAllele™ Automation sequencer data file peak height database analysis

Case Interpretation TrueAllele™ Automation sequencer data file peak height database report analysis interpretation

No Suspect Example Contributors A: Victim G: Unknown Suspect Samples A1 (Victim) C1 (Mixture) 70% A + 30% G 1 ng DNA, PowerPlex16, ABI/310

Simplicity Goals time < 1 minute time < 1 minute information full power information full power understandable yes understandable yes admissible yes admissible yes 1,440 minutes each day Backlog? (no suspect, convicted offender)

Conservative Exact Cautious Discriminating Power

26/2618/26 3.6x x10 11 Concordance: DP Synthetic: NIJ study comparisons Cases: 1,000 no suspect rape kits

Lab Process Scenarios screen data, organize case screen data, organize case second scorer review second scorer review post-conviction DNA testing post-conviction DNA testing service for DNA analyst service for DNA analyst NDIS forensic upload NDIS forensic upload NDIS profile download NDIS profile download mass disasters mass disasters serial crime serial crime advance troubleshooting advance troubleshooting …

Conclusions time time information information understandable understandable admissible admissible casework interpretation system: objective, unbiased, reproducible, objective, unbiased, reproducible, reliable, admissible, available reliable, admissible, available reports: simple, understandable studies: concordance, validation

Acknowledgements National Institute of Justice: Award #2001-IJ-CX-K003 Award #2001-IJ-CX-K003 Collaborators at: Florida, New York, Ohio, Pennsylvania, Florida, New York, Ohio, Pennsylvania, Virginia, NIST, Cellmark, UK FSS Virginia, NIST, Cellmark, UK FSS Cybergenetics: Developers, Quality Process, DNA Lab Developers, Quality Process, DNA Lab