SIRTs inhibitor: Sirtinol Supplementary Figure S3A Supplementary Figure S3 Sirtinol but not EX527 or PDF-170 significantly enhances ithe cytotoxic and.

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SIRTs inhibitor: Sirtinol Supplementary Figure S3A Supplementary Figure S3 Sirtinol but not EX527 or PDF-170 significantly enhances ithe cytotoxic and cytostatic function of dexamethasone in B-ALL cell lines REH and RS4;11 cells were treated with the a range of concentrations of SIRT inhibitors (0-100μM) and/or dexamethasone. Cell viability analysis was performed after 24, 48 and 72 h using MTT assay. A) Sintinol (SIRT1/2/6 inhibitor); B) EX527 (SIRT1 inhibitor); C) PDF-170 (SIRT2 inhibitor). (t-test: Sirtinol with 1μM dexamethasone verses dexamethasone at 1μM) *significant p<0.05, ** very significant p<0.01 and no marker: not significant.

SIRT1 inhibitor: EX-527 Supplementary Figure S3B Supplementary Figure S3 Sirtinol but not EX527 or PDF-170 significantly enhances ithe cytotoxic and cytostatic function of dexamethasone in B-ALL cell lines REH and RS4;11 cells were treated with the a range of concentrations of SIRT inhibitors (0-100μM) and/or dexamethasone. Cell viability analysis was performed after 24, 48 and 72 h using MTT assay. A) Sintinol (SIRT1/2/6 inhibitor); B) EX527 (SIRT1 inhibitor); C) PDF-170 (SIRT2 inhibitor). (t-test: Sirtinol with 1μM dexamethasone verses dexamethasone at 1μM) *significant p<0.05, ** very significant p<0.01 and no marker: not significant.

SIRT2 inhibitor: PDF-170 Supplementary Figure S3C Supplementary Figure S3 Sirtinol but not EX527 or PDF-170 significantly enhances ithe cytotoxic and cytostatic function of dexamethasone in B-ALL cell lines REH and RS4;11 cells were treated with the a range of concentrations of SIRT inhibitors (0-100μM) and/or dexamethasone. Cell viability analysis was performed after 24, 48 and 72 h using MTT assay. A) Sintinol (SIRT1/2/6 inhibitor); B) EX527 (SIRT1 inhibitor); C) PDF-170 (SIRT2 inhibitor). (t-test: Sirtinol with 1μM dexamethasone verses dexamethasone at 1μM) *significant p<0.05, ** very significant p<0.01 and no marker: not significant.