Translation 7.3. Translation the information coded in mRNA is translated to a polypeptide chain.

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Translation 7.3

Translation the information coded in mRNA is translated to a polypeptide chain

Transfer RNA (tRNA) nucleotides long some double-helical segments cloverleaf shape anticodon (sequence of 3 bases) is complementary to the codon on mRNA

Aminoacylatio the addition of amino acids to the 3’ end by aminoacyl-tRNA synthetase enzymes aminoacyl-tRNA = tRNA with corresponding amino acid attached

Wobble Hypothesis tRNA can recognize more than one codon by flexible pairing between the first (5’) base of the anticodon and third (3’) base of the codon

Ribosomes two subunits: large and small subunits clamp the mRNA between them

Ribosome Binding Sites A site (aminoacyl site): incoming aminoacyl tRNA binds here P site (peptidyl site): where tRNA with the growing polypeptide is E site (exit site): where tRNA leaves the ribosome

Translation initiation elongation termination

Initiation the ribosome recognizes the 5’ cap of the mRNA AUG (methionine) is always the first codon to ensure the correct reading frame

Initiation

Elongation the initiator tRNA (with methionine) enters the P site tRNA with the second amino acid enters the A site peptide bond forms between the methionine and the second amino acid (enzyme peptidyl transferase)

Elongation the ribosome shifts one codon –methionine tRNA is released (E site) –second tRNA moves to the P site –third tRNA enters the A site peptide bond is formed between the second and third amino acids elongation continues on in this manner

Elongation

Termination stop codons: UGA, UAG and UAA release factor protein binds to A site, polypeptide is released from P site, and the 2 subunits of the ribosome separate

Animations Narrated animation with quiz: html Narrated animation (McGraw-Hill) with quiz: hill.com/sites/ /student_view0/cha pter3/animation__how_translation_works.htm l

Eukaryotes vs. Prokaryotes polysome = complex formed when multiple ribosomes attach to the same mRNA in prokaryotic cells, translation and transcription can happen at the same time

Polypeptide to Protein the polypeptides are folded into their final protein form other modifications include addition of molecules such as sugars, assembling of mutiple polypeptide chains, etc.