Egal Gorse Transfection of Recombinant Plasmid DNA From Immunotoxins into Eukaryotic Cells for the Development of an Antibody Targeted Cancer Therapy

Goals To determine if mammalian carrier cells would accept and express immunotoxin DNA. To evaluate which carrier cells most effectively expressed immunotoxin DNA.

Cancer Cancer is the leading cause of death in the world (7.6 million per year). There is no effective treatment for cancer. Chemotherapy is inefficient (resistance and not targeted). Immunotoxins have shown promise as an effective cancer therapy (Vallera 2005).

AT B Binding Inside Outside Golgi Apparatus Endoplasmic Reticulum How EpCAMKDEL Kills Cells Cytosol

AT B Inside Transport Vesicle Outside Golgi Apparatus Endoplasmic Reticulum How EpCAMKDEL Kills Cells

B A T Cytosol Inside Outside Endoplasmic Reticulum Golgi Apparatus Transport Vesicle How EpCAMKDEL Kills Cells

B T T A Cytosol Inside Outside Endoplasmic Reticulum Golgi Apparatus Transport Vesicle How EpCAMKDEL Kills Cells

B T T A Cytosol Inside Outside Endoplasmic Reticulum Golgi Apparatus Transport Vesicle How EpCAMKDEL Kills Cells

B T T A Golgi Apparatus Endoplasmic Reticulum Inside Outside Cytosol How EpCAMKDEL Kills Cells

T B Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

T A T A Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

Cytosol Transport Vesicle T A Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells Golgi Apparatus

T A Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

T A Cytosol Golgi Apparatus Endoplasmic Reticulum Inside Outside How EpCAMKDEL Kills Cells

Conventional Immunotoxin Administration

Therapy Targeted Directly at The Site Of The Tumor Cells Transfected With Immunotoxins Directly at the Site of The Tumor

Transfecting EpCAMKDEL Immunotoxin DNA Into Cancerous Cells Using LNCX.NGFR

NIH-3T3 Transfected with EpCAMKDEL/LNCX.NGFR

Proliferation Assay

Conclusions Immunotoxin genes were capable of transfection into mammalian cells. The mammalian carrier cells were capable of expressing the immunotoxin genes. Expressed immunotoxins were capable of killing cancerous cells. The mouse cell lines tested were more capable of transfection than the human cell lines.

Future Study Testing in a live model (in vivo). Use results in an effective cancer therapy for human.

Acknowledgments Team Research Dr. Daniel Vallera Dr. Deborah Todhunter Ms. Lois Fruen

Egal Gorse Transfection of Recombinant Plasmid DNA From Immunotoxins into Eukaryotic Cells for the Development of an Antibody Targeted Carcinoma Therapy