High resolution MRI at 21.1 T of the hippocampus and temporal lobe white matter in the differential classification of Alzheimer’s Disease & Diffuse Lewy Body Disorder P. Foroutan 1,2, M.E. Murray 3, S. Fujioka 4, K.J. Schweitzer 4, D.W. Dickson 3, Z.K. Wszolek 4 and S.C. Grant 1,2 1 Chemical & Biomedical Engineering, FAMU-FSU College of Engineering, 2 The National High Magnetic Field Laboratory, The Florida State University, Tallahassee, FL, USA; 3 Departments of Pathology & Neuroscience, and 4 Neurology, Mayo Clinic, Jacksonville, Florida, USA

Hypothesis High resolution quantitative MRI performed at high magnetic fields can provide novel pathological information about differential neurodegeneration in LBD and AD related to structural alterations and iron accumulation. E-Poster #3754 -High resolution MRI at 21.1 T of the hippocampus and temporal lobe white matter in the differential classification of Alzheimer’s Disease & Diffuse Lewy Body Disorder

Differentiation of dementia  4.6 million new cases of dementia worldwide annually  Two most common types: Alzheimer’s Disease (AD) & Diffuse Lewy Body Disorder (DLBD)  Characteristics: irreversible, progressive, affects mostly males 60 years and older  Symptoms: decline of cognitive functions (learning & memory, language etc.) ADDLBD Characteristics:  -sheet amyloid (Ab) plaques & neurofibrillary tangles  -synuclein aggregates Location: Entorhinal cortex (hippocampus & amygdala) dispersed (spares hippocampus & amygdala) Previous Research: atrophy of hippocampus, linked to iron disperse atrophy gray matter, linked to iron E-Poster #3754 -High resolution MRI at 21.1 T of the hippocampus and temporal lobe white matter in the differential classification of Alzheimer’s Disease & Diffuse Lewy Body Disorder

Average T 2 and T 2 * times of the HC * Indicates statistical significance in a one-way ANOVA with LSD test (p<0.05) * * * * * * * * ** * * E-Poster #3754 -High resolution MRI at 21.1 T of the hippocampus and temporal lobe white matter in the differential classification of Alzheimer’s Disease & Diffuse Lewy Body Disorder

Conclusions  T 2 and T 2 * quantification of temporal lobe substructures proves a useful measure for the differential diagnosis of AD and DLBD.  In addition, ADC shows significant differences between AD and Controls.  Most promising biomarkers are the parahipocampal gyrus and temporal lobe white matter, which showed differing group patterns of T 2 and T 2 *.  Lower T 2 and T 2 * relaxation in the PHG is more likely a LBD case compared to either AD or normals whereas, higher T 2 and T 2 * values in the white matter would more likely coincides with AD.  T 2 times significantly correlated with vacuolation, which is not a pathologic entity, but does reflect the loss of cells or neuropils. E-Poster #3754 -High resolution MRI at 21.1 T of the hippocampus and temporal lobe white matter in the differential classification of Alzheimer’s Disease & Diffuse Lewy Body Disorder

Take Home Summary  AD classification correlates with increased T 2 /T 2 * and ADC for all deviations from control while DLBD correlates with decreased relaxivity Acknowledgements  The National High Magnetic Field Laboratory  FAMU-FSU College of Engineering Funding provided by: ◦ FSU (CRC-MDS) ◦ NHMFL (NSF DMR ) E-Poster #3754 -High resolution MRI at 21.1 T of the hippocampus and temporal lobe white matter in the differential classification of Alzheimer’s Disease & Diffuse Lewy Body Disorder