PHOTOSYNTHSIS: uses CO 2 as a carbon source and light as an energy source directly and indirectly supplies energy to most living organisms synthesizes energy rich organic molecules from energy poor molecules like H 2 O and CO 2

LEAVES LEAVES are the major organs of photosyn- thesis in plants.

CHLOROPLASTS: * plastid containing chlorophyll

PHOTOSYNTHESIS: 6 CO H 2 O + light C 6 H 12 O O H 2 O

LIGHT REACTIONS: Convert light energy to chemical bond energy in ATP and NADPH occurs in thylakoid membrane reduce NADP+ to NADPH Nicotinamide adenine dinucleotide phosphate (coenzyme) give off O 2 as a by product of splitting H 2 O generate ATP

LIGHT: Sun radiates a full spectrum of electromagnetic energy atmosphere screens out most, other than visible light I.R.U.V. this visible range of light drives photosynthesis

Blue and red wavelengths are most effectively absorbed by chlorophyll. Green is useless b/c green light is reflected back.

Chlorophyll a is the primary plant pigment Does use accessory pigments to help increase the absorption spectra

Chlorophyll b Carotenoids

Chlorophyll = pigment with a specific absorption spectrum Can be determined with a spectrophotometer When a pigment absorbs photons of light, the e- jump from ground state to excited state * This is unstable so they fall back  releasing E. Thylakoids trap released energy.

Pigments are clustered together. Only one chlorophyll molecule can initiate the transfer of excited e- to start light reactions = reaction center Other pigment molecules act as a light gathering antennae.

PHOTOSYSTEM I: reaction center is P700 Absorbs best at 700 nanometers CYCLIC ELECTRON FLOW: Uses only Photosystem I Generates ATP w/o producing NADPH or making O 2 cyclic b/c excited e- leave chlorophyll a at the reaction center and return to the reaction center

absorption of two photons of light (e- travel in pairs)  a series of REDOX reactions Occurs in the thylakoid at each step in the ETC, e- lose potential E until they return to ground state

PHOTOSYSTEM II: reaction center is P680 Absorbs best at 680 nanometers NONCYCLIC ELECTRON FLOW: Uses both photosytem I and II to transform light energy into chemical energy stored in NADPH and ATP

occurs in thylakoid passes e- from H 2 O to NADP+ produces ATP by non-cyclic photophosphorylation produces NADPH produces O 2

Light excites e- from P700 e- do not return to the reaction center, but are stored as high energy in NADPH oxidized chlorophyll becomes an oxidizing agent and its “e- holes” must be filled Photosystem II supplies e- to P700 to fill these “holes”

e- ejected from the P680 are trapped by the photosystem II primary e- acceptor e- are transferred to the primary e- acceptor by the same path used in cyclic e-flow as the e- move down the ETC, they lose PE until they reach ground state at P700 e- fill vacancies left in PS I when NADP+ is reduced

HOW WATER IS SPLIT: As e- flow from P680 to P700, e- are restored to P700, but this leaves P680 reaction center missing e-. a water splitting enzyme extracts e- from water and passes them to oxidized P680 P680 has a great affinity for e- PHOTOLYSIS

as water is oxidized, the removal of e- splits water into two hydrogen ions and an oxygen atom the oxygen atom combines with a second oxygen atom to form O 2 Released as a by- product

DARK REACTIONS: Carbon Fixation 1. Each molecule of CO 2 is attached to a 5-C sugar = ribulose biphosphate (RuBP) reaction is catalyzed by the enzyme RuBP carboxylase RUBISCO product is an unstable 6-C intermediate that becomes two molecules of 3-phosphoglycerate CALVIN BENSON Cycle

* For every 3 molecules of CO 2 that enter the Calvin cycle (via RUBISCO), 3 molecules of RuBP are carboxylated 6 molecules of 3- phophoglycerate are formed

2. Each molecule of 3-phospho- glycerate is phosphorylated by an enzyme that transfers a phosphate group from ATP. produces 1,3-diphosphoglycerate * For every 3 molecules of CO 2 entering the cycle, 6 molecules of ATP must be used to make 6 molecules of 1,3 diphospho- glycerate

3. 1,3-diphosphoglycerate has unstable phosphate bonds so it is ready for the addition of high energy e- donated from NADPH 1,3-diphosphoglycerate is reduced to glyceraldehyde phosphate (by hydrolysis of the phosphate bond)

e- from NADPH reduce the carboxyl group of 3-phospho- glycerate to the carbonyl group of glyceraldehyde phosphate stores more PE glyceraldehyde phosphate is a 3-C sugar (same sugar is produced from the splitting of glucose during glycolysis

So far in the Calvin cycle: 3 molecules of CO 2 have produced 6 molecules of glyceraldehyde phosphate * Only 1 molecule of 3C sugar can be counted as a net gain of carbohydrate.

4. A complex series of reactions rearrange the carbon skeletons of 5 molecules of glyceraldehyde phosphate into 3 molecules of RuBP. reactions require 3 molecules of ATP RuBP is regenerated and can receive CO 2 to begin cycle again

PHOTORESPIRATION: Process that uses O 2, makes CO 2, uses no ATP, and decreases photosynthesis. active site on RUBISCO can accept O 2 as well as CO 2 produces no ATP decreases photosynthesis by reducing organic molecules needed in Calvin cycle

* When O 2 concentration in the leaf is higher than the CO 2 concentration, RUBISCO accepts O 2 and transfers it to RuBP. fostered on dry, hot days when plants close stomates to prevent dehydration due to water loss from the leaf

If photorespiration can be reduced, some plants would increase crop yields b/c plants would not lose valuable CO 2. May be a result from earlier times when atmosphere contained less O 2 and more CO 2 RUBISCO’s active site developed an inability to distinguish between O 2 and CO 2

C 4 PLANTS: plants that incorporate CO 2 into 4-C compounds (instead of 3-C 3-phosphoglycerate = C 3 plant) ex. corn, sugar cane Allows plants to fix CO 2 under conditions that favor photo- respiration.

1. CO 2 is added to phosphophenol pyruvate (PEP) to form oxaloacetate (4C) PEP carboxylase adds CO 2 to PEP (compared to RUBISCO it has a much better affinity for CO 2 and NO affinity for O 2 )

2. After CO 2 has been fixed by mesophyll cells, they convert oxaloacetate to another 4-C compound (usually malate)

3. Mesophyll cells export malate to bundle sheath cells through plasmodesmata. in the bundle sheath cells, malate releases CO 2 which joins with RuBP by RUBISCO and goes through Calvin cycle mesophyll cells pump CO 2 into bundle sheath cells, preventing photorespiration

CAM PLANTS: Crassulacean Acid Metabolism ex. succulent plants adapted to arid conditions (cacti) usually open stomates at night and close them during the day (opposite of most plants) Helps them to conserve water but prevents CO 2 from entering leaves

when stomates are open at night CO 2 is taken up and incorporated into a variety of organic acids = crassulacean acid organic acids are stored in vacuoles in the mesophyll until morning, when stomates close during the day, the light reactions supply ATP and NADPH