Lecture-7 Genome editing CRISPR

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Presentation transcript:

Lecture-7 Genome editing CRISPR Huseyin Tombuloglu, Phd GBE423 Genomics & Proteomics

Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins CRISPR/Cas9 System

What is it? derived from a natural process found in bacteria to protect themselves from pathogens targets genes for editing and regulating comparable to Photoshop Horizon Licenses Harvard University Gene-Editing Technology. (2013). Drug Discovery & Development.

To initiate gene modification, sgRNA (single guide RNA) Cas9 complex Cas9 nuclease

Protospacer Adjacent Motif (PAM) Target Sequence Gene of Interest

Non-Homologous End Joining (NHEJ) DNA repair pathway Stop Codon

Summation: crispr/Cas system sgRNA/Cas9 complex binds to gene Cas breaks gene (double strand breaks) Induced mutation in gene sequence altered gene sequence  dysfunctional CAD gene