Study Design UIHC HIV Clinic Patients Invitation – Informed Consent Heparinized Blood/Serum Obtained CD4, HIV VL measured Serum => GBV-C VL Cells => flow.

Slides:

Advertisements

Similar presentations

NATURAL KILLER CELLS WAYNE BORCHERDING MT(ASCP). NATURAL KILLER CELLS u DEVELOPMENT u RECEPTORS u FUNCTION u LABORATORY ANALYSIS.

Advertisements

Myeloid dendritic cells and HIV latency in resting T-cells Nitasha A Kumar, Vanessa A Evans, Suha Saleh, Candida de Fonseca Pereira, Paula Ellenberg, Paul.

Lecture: HIV I.Motivation What can we learn when we apply evolutionary principles to our understanding of the of the HIV epidemic?? Can we use HIV to introduce.

Project 2: Cellular Mechanisms and Interaction of Neurokinin-1 Receptor (NK- 1R) Antagonists and HIV Co- Receptors CCR5 and CXCR4 Principal Investigator.

Cigarette Smoke Extract Suppresses Human Dendritic Cell Function Leading to Preferential Induction of Th-2 Priming 高丰光 Robert Vassallo The Journal of Immunology,

THE IMMUNOPATHOGENESIS OF HIV INFECTION Lymphotropic virus.

Figure 2. Overview of HIV infection of a

M1 – Immunology CYTOKINES AND CHEMOKINES March 26, 2009 Ronald B

Volume 94, Issue 2, Pages (March 2014)

Suppl Figure 1 NSCLC OvCa A B

Pitavastatin-induced downregulation of CCR2 and CCR5 in monocytes is associated with the arrest of cell-cycle in S phase Masahiro Fujino, Shin-ichiro.

Copyright © 2005 American Medical Association. All rights reserved.

a b c Figure S1 Figure S1: Gating strategy describing T cell subsets.

Katsin MA, Vitebsk State Medical University (Belarus)

Joana R. Guedes, Isabel Santana, Catarina Cunha, Diana Duro, Maria R

Chronic immune activation in HIV associated Non Hodgkin lymphoma and the effect of antiretroviral therapy Brian Flepisi University of the Western Cape.

Andrew E. Williams. , Ricardo J. José, Paul F. Mercer, Jeremy S

The Effect of Antiretroviral Naïve HIV-1 Infection on the Ability of Natural Killer Cells to Produce IFN-γ upon Exposure to Plasmodium falciparum-Infected.

Flow cytometry imaging identifies rare TH2 cells expressing thymic stromal lymphopoietin receptor in a “proallergic” milieu Amanda J. Reefer, MS, Kathryn.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy Yin Yao, MD, Cai-Ling.

Monocyte chemoattractant chemokines in cystic fibrosis

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection

Primary immunodeficiency diseases associated with increased susceptibility to viral infections and malignancies Nima Rezaei, MD, PhD, Mona Hedayat, MD,

William A. Comrie, PhD, Aiman J

Diminished suppressive potency of primary Treg clones from IPEXA384T patients. Diminished suppressive potency of primary Treg clones from IPEXA384T patients.

Lineage-Specific Expression of Human Immunodeficiency Virus (HIV) Receptor/Coreceptors in Differentiating Hematopoietic Precursors: Correlation With Susceptibility.

Potential roles of cytokines and chemokines in human intervertebral disc degeneration: interleukin-1 is a master regulator of catabolic processes K.L.E.

Volume 141, Issue 1, Pages e2 (July 2011)

Differentiation and Functions of CD8+ Effector T Cells

Interleukin (IL)-1β-producing cells in peripheral blood mononuclear cells (PBMCs). Interleukin (IL)-1β-producing cells in peripheral blood mononuclear.

Volume 143, Issue 4, Pages e8 (October 2012)

Volume 144, Issue 7, Pages e10 (June 2013)

Chemokines, Chemokine Receptors, and Allograft Rejection

HIV infection en route to endogenization: two cases

FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.

Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper by Haifa Hamdi, Véronique.

Volume 59, Issue 3, Pages (September 2013)

Hepatitis C and Lymphoproliferative Disorders: From Mixed Cryoglobulinemia to Non- Hodgkin's Lymphoma Lenna A. Martyak, Melina Yeganeh, Sammy Saab Clinical.

Clinical and biological significance of stem-like CD133+CXCR4+ cells in esophageal squamous cell carcinoma Chunlai Lu, MD, Fengkai Xu, MD, Jie Gu, MD,

Depo-Provera altered the expression of cell surface markers associated with HIV susceptibility. Depo-Provera altered the expression of cell surface markers.

Volume 37, Issue 1, Pages (July 2012)

Differential expression of functional chemokine receptors on human blood and lung group 2 innate lymphoid cells Cathryn A. Weston, PhD, Batika M.J. Rana,

Volume 46, Issue 6, Pages e4 (June 2017)

Dysregulation of proinflammatory versus anti-inflammatory human TH17 cell functionalities in the autoinflammatory Schnitzler syndrome Rebecca Noster,

Therapeutic Action of Ghrelin in a Mouse Model of Colitis

Tamar Hermesh, Bruno Moltedo, Thomas M. Moran, Carolina B. López

Postoperative changes in blood monocyte subsets.

Figure 1 Schematic overview of flow cytometry Schematic overview on the analysis of peripheral immune cells by flow cytometry. Schematic overview of flow.

Volume 143, Issue 6, Pages e8 (December 2012)

Indiscriminate Memories during Infection Control

Chen Yao, BS, Sandra M. Zurawski, PhD, Elizabeth S

Sabah El-Ghaiesh, MSc, Joseph P

Lisa P. Daley-Bauer, Grace M. Wynn, Edward S. Mocarski Immunity

IL-7 Knocks the Socs Off Chronic Viral Infection

(A) Reduction of circulating stromal cell-derived factor (SDF)-1 levels over time in patients with rheumatoid arthritis (RA) and ankylosing spondylitis.

Hematopoietic potential of HEP from patient-specific iPSC may be reduced. Hematopoietic potential of HEP from patient-specific iPSC may be reduced. (A)

Sara Paveglio, PhD, MS, Erin Bennett, MS, Kelly L. Hawley, PhD, Adam P

Comparison of conventional NSG and hIL-7 expressing NSG humanized mice

Bradykinin increases the in vivo expression of the CXC chemokine receptors CXCR1 and CXCR2 in patients with allergic rhinitis Jane Eddleston, PhD, Sandra.

Expression of fractalkine and its receptor, CX3CR1, in atopic dermatitis: Possible contribution to skin inflammation Takeshi Echigo, MD, Minoru Hasegawa,

Figure 2. Immunophenotypic characteristics of Gal-4-treated monocytes

CD56brightCD16− natural killer cells accumulate in the ovarian follicular fluid of patients undergoing in vitro fertilization Ofer Fainaru, M.D., Ph.D.,

Inflammation Mediated by JNK in Myeloid Cells Promotes the Development of Hepatitis and Hepatocellular Carcinoma Myoung Sook Han, Tamera Barrett, Michael.

PD-1 inhibition stimulates the proliferation and cytokine secretion of exhausted/senescent CD8+ T cells in vitro. PD-1 inhibition stimulates the proliferation.

Melanoma patient monocytes have altered expression of inflammatory and surface markers. Melanoma patient monocytes have altered expression of inflammatory.

Increase in proliferation and activation of immune cells in peripheral blood after NKTR-214 treatment. Increase in proliferation and activation of immune.

SY-1425 induces maturation in RARA-high AML

IL-9–expressing TH cells are highly enriched in CCR4+/CCR8+ effector memory TH cells. IL-9–expressing THcells are highly enriched in CCR4+/CCR8+effector.

Volume 12, Issue 5, Pages (November 2005)

Surface expression of chemokine receptors on M

Presentation transcript:

Study Design UIHC HIV Clinic Patients Invitation – Informed Consent Heparinized Blood/Serum Obtained CD4, HIV VL measured Serum => GBV-C VL Cells => flow cytometry Does GBV-C alter B cell & NK cell activation or alter monocyte CCR5/CXCR4 expression? GBV-C blunts T cell activation & proliferation GBV-C induces chemokines GBV-C CCR5 and CXCR4 on CD4 cells GBV-C replicates in T and B cells P=0.01 N=11N=13N=11N=9 ns

P=0.018 P=0.009 B cell activation: GB+ < GB- p=0.024 HIV Viremic GB+ < GB- p=0.018 NK cell activation: CD56dim/CD16+: NS CD56brt/CD16-: If HIV RNA neg: NS If HIV RNA pos: GB+ < GB- p=0.009

Monocyte CCR5 expression GB+ < GB- p=0.009 HIV RNA+: GB+ < GB- p=0.03 CXCR4 GB+< GB-, but NS P=0.03 Conclusion: GBV-C replication in vivo Diminishes B cell activation Blunts CD16+ NK cell activation Downregulates monocyte CCR5 GBV-C replicates in T and B cells; however, replication limited to a very small percentage of cells. Replication has not been shown in NK cells or monocytes. Effect in these cells may be due to induction of chemokines and/or cytokines The effect of GBV-C activation on T and B lymphocytes, NK cells and monocytes may contribute to prolonged survival in HIV- infected individuals.