DNA & Forensic Evidence Lab

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Presentation transcript:

DNA & Forensic Evidence Lab

DNA & Forensic Science Lab Predictions: Is DNA found in the food we eat? Where exactly do you think DNA is located? Can DNA be extracted? Why is DNA extraction an important skill for a forensic scientist?

How does forensic id work? Every species has their own DNA sequence Scan 13 different regions (loci) when creating a human DNA profile Human DNA Profile=DNA Fingerprint

Examples of DNA uses in forensic science DNA left at a crime scene Exonerate persons who are wrongfully accused of crimes Identify crime and catastrophe victims Establish paternity and other family relationships

Is dNA effective in id of persons? DNA id is effective if used intelligently. Example: Assume that type O blood is found at crime scene. Type O occurs in about 45% of Americans. If investigators type only for ABO, finding the suspect with type O blood doesn’t reveal much. It’s usually a chain of biological and physical evidence like hair, footprints that leads to the making of the match.

What are some of the dna technologies used in forensics? RFLP Restriction Fragment Length Polymorphism PCR Analysis Polymerase Chain Reaction STR Analysis Short tandem repeat technology Mitochondrial DNA Analysis *related to the DNA loci passed on from the mother Y-chromosome analysis *passed on loci from the father

Interesting Uses of DNA in forensic Identification Identifying September 11th victims Migrational Patterns of Human throughout history DNA Banks for Endangered Species Poached Animals

Materials & Function Strawberry: large genome due to the fact that it is an octoploid (8 times the amount of genetic info.) Soap: dissolve the phospholipid bilayers of the membrane and organelles (plant cell) Salt: break up the protein chains that bind the nucleic acids Ethanol: is kept cold on ice because the DNA is insoluble making it easy to separate from the beaker.

conclusion Online is posted the conclusion worksheet. Answer all questions on your own sheet of paper. Attach your answers to the rest of your lab report. Lab report due Wednesday.