ORDINATION What is it? What kind of biological questions can we answer? How can we do it in CANOCO 4.5? Some general advice on how to start analyses.

How different or similar is the vegetation at these two places? What are the patterns within each of them? Biomass Productivity Diversity Species composition

Ordination Analyses of data with many response variables Search for patterns We can also quantify and test the effect of one or many predictor variables (tomorrow!!)

But first: do communities exist?

A short answer after a long debate: No. Compositional variation in nature tends to be gradual.

How can we analyse species composition? PinusTsuga Site 1310 Site 251 Site 302 Site 448 Site Within some defined environment or area we sample a number of plots and register the species present

Pinus Tsuga Site 1 Site 3 Site 2 Site 4 Site 5 PinusTsuga Site 1310 Site 251 Site 302 Site 448 Site AcerBetula SPECIES SPACE

Site 1 site Acer Tsuga Betula Pine PinusTsuga Site 1310 Site 251 Site 302 Site 448 Site AcerBetula Site space

Data dimensions The sites differ in species abundances Each species is a variable – a dimension – –in a dataset with n species the differences between plots can be described exactly by their positions in a n-dimensional space Species are not distributed independently of each other –They respond to the same factors, affect each other… Can we somehow find a few dimensions that capture the bulk of the compositional information?

Pinus Tsuga Site 1 Site 3 Site 2 Site 4 Site 5

Pinus Tsuga Site 1 Site 3 Site 2 Site 4 Site 5

Site 1 Site 3 Site 2 Site 4 Site 5

10 This line describes the relative positions of sites along one dimension that captures the largest fraction possible of the variation in species composition We have done a Principal Component Analysis!!!!

Linear vs. Unimodal methods In the examples above we assumed that species abundance and the environment is linearly related This is sometimes true! (when we are within a ca SD ’window’ along an environmental gradient)

Linear vs. Unimodal methods But what if we want to analyse the whole gradient? A linear-based method will give a ’wrong’ solution! (which would give us a statistical artifact called the ’horseshoe effect’) There are unimodal-based methods (CA, DCA, …)

Correspondence analysis (CA) when the response is unimodal Sample where the species is present. (size indicates abundance) Weigthed average optimum of this species

In the same way you can find the optimum of a sample: the weighted average of the species it contains Species present in the sample. (size indicates abundance) Weigthed average optimum of the sample

Weighted averaging species scores are weighted averages of site scores –the weights are related to how common the species are in the sites site scores are weighted averages of species scores –the weights are (again) related to how commmon the species are in the sites ITERATIVE METHOD!

The arch problem After the first CA axis is constructed, the program will start ’looking for’ a second, uncorrelated axis. If no ’real’ gradient exists in the data, it will tend to ’find’ the folded axis 1 (which by definition uncorrelated, and half the lenght of the first axis)

Identifying the arch problem …and handling it The problem is easily identified by inspecting – The CA ordination diagram can you see an arch in the plot positions along axis 2? –The eigenvalues of the first and second axes Is the eigenvalue of axis 1 ca. 2* that of axis 2) The problem can be removed by detrending –Detrend by segments in indirect methods

The magic behind the ordination diagrams PCA CA

Biplot interpretation Species and sample positions along the axes can be presented as ordinaion diagrams These diagrams tell us something about the species composition the samples Interpretation differs between ordination diagrams from linear methods (PCA) and unimodal methods (CA)!

PCA

Etc

CA Decreasing probability of occurrence

CA Decreasing probability of occurrence

Summary unimodal vs. linear methods detrending in unimodal methods biplot vs. centroid interpretation