Quantitation part 9 By : Saib Al Owini.

Slides:



Advertisements
Similar presentations
Lab 3 Cell Biology 2015, Kristina Ruuth
Advertisements

Bacterial Generation Time
Cell Division in Microgravity
Introduction to Cell Culture Biotechnology I. Cell Culture Definition: the in vitro growth of cells isolated from multi-cellular organisms Process: Cells.
Combined Effect of Ferric Chloride and Mercuric Chloride on the Growth of Cyanobacteria Anacystis nidulans By Ted Handler, Farrell Parker, Alex Lewis,
Cell proliferation assay versus cell viability assays Lecture 4.
Tissue Culture Methodology. Historical Perspective Early 20 th Century Technique Antibiotics and Laminar Flow Hoods Allow for Tissue Culturing to Grow.
..Counting Bacteria.. Made By: Duaa Mohammed EL- Boh. ID: Supervised By: DR. Abdelraouf Elmanama.
Faculty of Medicine and Health Sciences Microbiology Lab Second semester 2014 prepared by: Mohammad Al-Qadi
Counting Bacteria.
JACK LEECH GRADE 12 PITTSBURGH CENTRAL CATHOLIC HIGH SCHOOL PJAS 2011 Estradiol Effects on a Mammalian Stem Cell Line.
Lab-1 Studying Death at Cell level Behzad Khoshnood, 2015.
Counting Bacteria.
The Effects of UVC Light on C2C12 Stem Cells Cory Soltys Pittsburgh Central Catholic High School Grade 12 PJAS 2015.
Microarrays (Gene Chips) Pioneered by Pat Brown in mid 1990’s To monitor thousands of mRNAs simultaneously Comparative Northern blot on thousands of genes.
James Constantin Central Catholic High School Grade 11.
The Impact of Red Food Coloring on C2C12 Stem Cells Christian Ford Grade 12 Central Catholic High School PJAS 2015.
Basic technique Training and and Practice  Pipetting and transfer of fluid  Observation of cultured cells  Aseptic technique: preparation of mediums.
1 Neil Carleton Grade 11 Pittsburgh Central Catholic High School PJAS 2012.
The Effect of Bisphenol A on the Growth of Brest Cancer Cells
FIGURE 13.1 Clonal Cell Yield. Relationship of the cell yield in a clone to the number of population doublings; for example, 20 doublings are required.
Cell Culturing and Fermentation Biotechnology II.
Experiment of cytobiology. schedual cell culture and counting cell isolation of subcellular structure cell proliferation assays methods in apoptosis.
Chapter 21 Quantitation Cell Counting Technique 1.Hemocytometer 2.Electronic Cell Counter.
Growth of bacteria in culture
1. MIKROSCOPE And 2.HEMOCYTOMETER
Cell Culture Techniques
MTT ASSAY Induction of Cell Proliferation by ConA.
Plant and Mammalian Tissue Culture Counting Cells and Experimental Design.
Microscope Parts Quiz Multiply the lenses together to get the total magnification. A. B. C. D. E. F. G. H. I. eyepieceilluminatorstagearm fine focus coarse.
Essential Laboratory Equipment for Cell Culture Dr. Khalid Enan DFG-Sponsored Workshop, Assuit University, Egypt 10 Sep 2012.
Learning Targets “I Can…” -Focus a slide under a microscope by always starting with the objective (the red) to observe living yeast cells. -Predict the.
XENOESTROGEN EFFECTS ON STEM CELL BEHAVIOR Maria DeRenzo Oakland Catholic High School Grade 10.
RBC, WBC, & PLT Counts Nada Mohamed Ahmed, MD, MT (ASCP)i.
RBC, WBC, & PLT Counts.
FIGURE 21.1 Clonogenic Assay for Adherent Cells. Cells are trypsinized, counted, and diluted as for monolayer dilution cloning (see Protocol 13.1). The.
WBC manual count using haemocytometer.  Introduction The blood consist of 3 type of specialized cellular elements:  Erythrocytes -RBCs  Leukocytes.
Enumeration (determine the numbers of bacteria in a sample) Direct Measurement of Microbial Growth  Microscopic count - the microbes in a measured volume.
WBC manual count using hemocytometer
By: Zaib Momin. Synthroid is used by many children and also adults whose thyroid gland isn’t supplying enough hormones to keep overall metabolism in working.
Cell count with Hemocytometer
MTT Cell Proliferation Assay sunpingli
STUDY OF NEUBAUER’S CHAMBER Fahareen-Binta-Mosharraf MNS.
Correlation between the increased number of PML-NBs and the increased HBV DNA copy number in response to chemotherapy and irradiation-induced DNA damage.
Onion Root Mitosis. Background In plants, the roots grow as they search for water and nutrients. These regions of growth are good for studying the cell.
Cytoskeleton Lab 2: The Effects of Cytochalasin B on the Actin Cytoskeleton & Cell Viability in Mouse Fibroblast Cells.
Hemocytometer  For hematology, microbiology, cell culture, and many applications that require use of suspensions of cells it is necessary to determine.
Faculty of Medicine and Health Sciences
WBC manual count using hemocytometer
EQUIPMENTS.
Protocol of preparing Adhesion Assay imaging
Laboratory Technique LAB.
White Blood Cells Count Using Hemocytometer
The Effect of Bisphenol A on the Growth of Brest Cancer Cells
Using a haemocytometer
Amanda M. Nelson, Kathryn L. Gilliland, Zhaoyuan Cong, Diane M
Counting cells using a haemocytometer
Measurement of cell growth by direct methods
Volume 5, Issue 4, Pages (April 1995)
Metode Manual (Bergeys manual of bacteriology) sni.
S. Varghese, Ph. D. , P. Theprungsirikul, B. S. , S. Sahani, B. S. , N
BELL RINGER A student grew a yeast culture on sterilized nutrient medium in a closed dish for 5 days. Each day, she took the same size sample from the.
Supplemental Figure 1A – I
Supplemental Figure % of viable cells 10 5 RHPS4 RHPS4+GPI
Measurement of cell growth by direct methods
The Effect of FGF on Mammalian Stem Cells
The Effect of Bisphenol A on the Growth of Brest Cancer Cells
The effect of different concentrations of WMC-79 and time of exposure on the growth of HCT-116 cells. The effect of different concentrations of WMC-79.
Characterization of Lkb1-WT and Lkb1-null cell lines.
BME treatment increases cytotoxic activity of NK3
Presentation transcript:

Quantitation part 9 By : Saib Al Owini

CELL COUNTING - Hemocytometer -Electronic Counting

PROTOCOL 21.1. CELL COUNTING BYHEMOCYTOMETER Outline Trypsinize a monolayer culture, or sample a suspension culture, prepare a hemocytometer slide, and add the cells to the counting chamber. Count the cells on a microscope and calculate the cell concentration. Materials Sterile: D-PBSA Crude trypsin, 0.25% Growth medium Yellow pipettor tips Nonsterile: Pipettor, 20 μL or adjustable 100 μL Hemocytometer (Improved Neubauer) Tally counter Microscope

H:\ملفات فيديوأنسجة\YouTube - hemacytometer count- sabrina lin.flv

Electronic Counting

CASY Electronic Cell Counter.

CASY Electronic Cell Counter.

Beckman Coulter Vi-CELL Electronic Cell Counter

CELL PROLIFERATION Experimental Design Growth Cycle

Cytotoxicity Viability

H:\ملفات فيديوأنسجة\YouTube - Trypan Blue part 3.flv

Metabolic Cytotoxicity Assays Microtitration Assays