Quantitation part 9 By : Saib Al Owini
CELL COUNTING - Hemocytometer -Electronic Counting
PROTOCOL 21.1. CELL COUNTING BYHEMOCYTOMETER Outline Trypsinize a monolayer culture, or sample a suspension culture, prepare a hemocytometer slide, and add the cells to the counting chamber. Count the cells on a microscope and calculate the cell concentration. Materials Sterile: D-PBSA Crude trypsin, 0.25% Growth medium Yellow pipettor tips Nonsterile: Pipettor, 20 μL or adjustable 100 μL Hemocytometer (Improved Neubauer) Tally counter Microscope
H:\ملفات فيديوأنسجة\YouTube - hemacytometer count- sabrina lin.flv
Electronic Counting
CASY Electronic Cell Counter.
CASY Electronic Cell Counter.
Beckman Coulter Vi-CELL Electronic Cell Counter
CELL PROLIFERATION Experimental Design Growth Cycle
Cytotoxicity Viability
H:\ملفات فيديوأنسجة\YouTube - Trypan Blue part 3.flv
Metabolic Cytotoxicity Assays Microtitration Assays