Quantitation part 9 By : Saib Al Owini.

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Presentation transcript:

Quantitation part 9 By : Saib Al Owini

CELL COUNTING - Hemocytometer -Electronic Counting

PROTOCOL 21.1. CELL COUNTING BYHEMOCYTOMETER Outline Trypsinize a monolayer culture, or sample a suspension culture, prepare a hemocytometer slide, and add the cells to the counting chamber. Count the cells on a microscope and calculate the cell concentration. Materials Sterile: D-PBSA Crude trypsin, 0.25% Growth medium Yellow pipettor tips Nonsterile: Pipettor, 20 μL or adjustable 100 μL Hemocytometer (Improved Neubauer) Tally counter Microscope

H:\ملفات فيديوأنسجة\YouTube - hemacytometer count- sabrina lin.flv

Electronic Counting

CASY Electronic Cell Counter.

CASY Electronic Cell Counter.

Beckman Coulter Vi-CELL Electronic Cell Counter

CELL PROLIFERATION Experimental Design Growth Cycle

Cytotoxicity Viability

H:\ملفات فيديوأنسجة\YouTube - Trypan Blue part 3.flv

Metabolic Cytotoxicity Assays Microtitration Assays

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