Cell-Mediated Cytotoxicity Cytotoxic T-Lymphocytes Natural Killer Cells Antibody-Dependent, Cell-mediated Cytotoxicity Delayed-type Hypersensitivity Folder Title: CellKill KillTitl Updated: November, 25, 2004

Cell-Mediated Immunity (CMI): Passive transfer from donor to recipient of cells alone, transfers the immune response function Response to: Neoplastic (cancer) Cells Infected or Altered Cells Intra-cellular Pathogens Fungi Allo-grafts and other transplants CMIRoles

Cell-Mediated Immunity (CMI): Types of Cytotoxic Cells Antigen-Specific T-Cells (Have TCR): Tc : Cytotoxic T-Cells TDTH : Delayed-Type Hypersensitivity Cells ("Type IV Hypersensitivity") Non-Antigen-Specific Cells: Macrophages NK Cells Granulocytes: Neutrophils, Eosinophils, Basophils and Tissue Mast Cells CMICells

CTL Antigen Recognition

CD4 & CD8 Sensitize & Effect

CTL Sensitizaton Phase

CTL & MHC

Effector Phase by CTL

CTL & ICAM-1

CTL binding to Target Cell Cytotoxic T-Cell Binding to Target Cell (figure 16-6, Kuby, 3rd Edition) TC = Target Cell for Cytolytic T-Cell Smaller Cell = CTL CTLandTC

CTL Perforin Pore

T-Cell Killing by Induction of Apoptosis (figure 16-8, Kuby, 3rd Edition) ICE = Interleukin 1 Converting Enzyme Signals Activation of Apoptosis CTL&Apop

T-Cell and NK Cell Development

Comparison of T- and NK Cells

Natural Killer Cell Targets and Restriction to Altered-Self (Figure 16-11, Kuby, 3rd Edition) No Killing (Recognized Self; MHC Class I) Target Cell Killed (Did not recognize self; low MHC Class I NKTarget

ADCC Targets

CD28 – B7 Co-stimulators

B7 Melanoma Vaccine