Integral spicule matrix proteins and BSA on Si and on calcite wafers, analyzed at SRC, Feb. 2011 The samples are labeled 48C, 72C, 48T, 72T, and BSA. All.

Slides:

Advertisements

Similar presentations

Abstract This paper is focused on the study of different functionalised nanostructurated silicon – porous silicon – layers with the aim to find the suitable.

Advertisements

PH and Indicators Chemistry Mrs. Coyle. Basic 14>ph>7 Neutral ph=7 Acidic 1

Learning outcomes:  To know what the pH scale is.  Know how to measure pH  Give examples of different acids and alkalis. Read page 56 and 57.

Abstract Wisconsin requires Bray P1 as the routine soil test phosphorus method for fertilizer recommendations and phosphorus loss risk predictions. Highly.

How Sedimentary Rocks are Formed. Sandstone In an area where sand is deposited.

Sample spotting techniques Dried droplet Crushed crystal Thin layer Sandwich.

Extraction of Nucleic Acids (Genomic DNA, mRNA and Plasmid DNA)

THE RELATIONSHIP BETWEEN H2CO3* AND HCO3-

Lecture 21 10/24/05 Seminar today.

% Calcium in Egg Shells By EDTA Complexometric Titration

Lecture 9 Acid-Base Equilibria -I. Strong acids: HCl HBr HI HNO 3 H 2 SO 4 HClO 4 (HO) n E=O Weak acid (HO) n EO 2 Strong acid We titrate with strong.

Lecture 24 10/31/05. Finding endpoint with pH electrode.

Lecture 20 10/19/05.

1 Chapter 8 Acids and Bases. 2 What is an Acid? In water, an acid increases the hydronium (H 3 O + ) concentration of an aqueous solution. Strong acids.

Acid-Base Titration Finding the amount of Acetyl Salicylic Acid in three different doses of Aspirin.

Soil Testing Lab. Purpose You will determine: 1.Soil composition 2.Soil pH 3.Soil Macronutrients.

Quantitative Chemical Analysis Seventh Edition Quantitative Chemical Analysis Seventh Edition Chapter 8-12 Acid-Base Titrations Copyright © 2007 by W.

By: Michael Zanecosky.  To determine the amount of cadmium in protein shakes and other metals which can have detrimental effects of the body.

PH Scale Standard: 5d-students know how to use the pH scale to characterize acid and base solutions.

Alkalinity Bicarbonate-carbonate. Alkalinity is… …the measure of the ability of a water to neutralize an acid.

ACIDS AND BASES. Acids  Acidic solutions contain water and hydrogen ions (H+)

Exp. 15: Volumetric Analysis: Total Hardness of Water by EDTA

The Chemistry of Acids and Bases. Acids and Bases: Calculate pH/pOH At the conclusion of our time together, you should be able to: 1. Change any pH reading.

Topic 3 Pages  Let’s list some that you have already used today! ◦ Shampoo and soap ◦ Orange juice ◦ Milk ◦ Water.

Types of Chemical Reactions and Solution Stoichiometry – Part 2

Molar Concentration. Measuring Concentration Molarity (M): the number of moles of solute in 1 liter solution. Example: 0.5 moles of NaCl dissolved in.

CH 223 LECTURE # 15 SAMPLE QUESTIONS. The lesson is for not only accuracy, but speed. You will always be under some time constraint. You can work in pairs.

1 Buffers & Titrations Chapter Buffers A soln that resists change in pH when strong acid or strong base is added A soln that resists change in pH.

Section 4: Acids and Bases in Solution Objectives: describe the ions formed when acids and bases are dissolved in water describe the pH scale and tell.

Chat with Fred and Chris Nov. 19 th, 2010 rhombohedra: SEM observation vs time, 24h-72h Bodega Marine Station payments - order Mytilus californianus spicule.

Development of Prostatic Exosomal Protein Lateral Flow Chromatographic Strips for Detection of Chronic Prostatitis Jiao Zhang MD, PhD. The Brody School.

Signal to Noise Comparison PEEM-3 and SPHINX Gilbert Group

Titration. Weak Acid/Strong Base Titration Curve pH starts higher Rapid rise and then levels into a zone where pH doesn’t change much (buffering zone)

Karst Chemistry II. Conductivity – Specific Conductance Conductance – the electrical conductivity of aqueous solution, and is directly related to the.

Titration and pH Curves..   A titration curve is a plot of pH vs. volume of added titrant.

Volumetric Analysis – Titrations Volumetric Analysis a process of analysis accomplished by adding together particular volumes of 2 solutions the objective.

 TITRATIONS.  What did one titration tell the other?  Let's meet at the endpoint.  Why are chemists great for solving problems?  They have all the.

Optical Density of Ge Implanted Type III Silica of various concentrations Optical Density of Ge Implanted Type IV Silica of various concentrations Optical.

Strengths of Acids and Bases Integrated Science II.

Acids and Bases Strengths of Acids and Bases Making Dilutions.

Titration. Definition Process used to determine the strength of an unknown acid or base Can determine the unknown acid by adding to it a base of known.

Buffering Capacity: Seawater CO 2 Chemistry Scripps Classroom Connectionhttp://earthref/SCC.

Stoichiometry: Quantitative Information About Chemical Reactions Chapter 4.

Analysis Questions: 1.What does pH measure? the concentration (not “amount”) of hydrogen (H+) ions in a solution. 2.Which pH has the highest concentration.

What is Alkalinity? the measurement of the buffering capacity of a body of water that is, a body of water’s resistance to the lowering of pH when acids.

Indicators And Titration. Indicators Usually organic acid or base whose color is sensitive to pH Indicator is weak acid or base In basic solution, indicator.

POINT > Review acid-base neutralization POINT > Identify pH indicators and how they work POINT > Describe titration process.

Titration Curves. Problem   50.0 mL of 0.10 M acetic acid (K a = 1.8 x ) are titrated with 0.10 M NaOH. Calculate the pH after the additions of.

Titration and pH Curves..   A titration curve is a plot of pH vs. volume of added titrant.

Lab Session 7 IUG, spring 2015 TMZ.

Fun fun. Acid-Base Reactions. Acid-Base Neutralization  Acids and bases will react with each other to form water and a salt.  Water has a pH of 7—it.

Titration A standard solution is used to determine the concentration of another solution.

Titration and pH Curves.

Local Way Of Detecting Soil PH To Improve Agriculture

Name: ___________________ ( ) Class: 2A

Name: ___________________ ( ) Class: 2A

Acid-Base Titration Titration is a chemical analysis involving the addition of a known concentration of titrant to a known volume but unknown concentration.

Calcium sulfate and carbonate co-precipitation

(Acid/Base neutralization)

Strong and Weak Acids and Bases

Bicarbonate-carbonate

LO: I understand how the pH of a salt solution can vary.

How Sedimentary Rocks are Formed

Volume 23, Issue 12, Pages (June 2013)

Lab Activity 7 IUG, Fall 2017 TMZ.

Lab Activity 7 IUG, Fall 2017 TMZ.

Lab Session 7 IUG, 2012 TMZ.

copyright cmassengale

How Sedimentary Rocks are Formed

Presentation transcript:

integral spicule matrix proteins and BSA on Si and on calcite wafers, analyzed at SRC, Feb The samples are labeled 48C, 72C, 48T, 72T, and BSA. All samples are at the concentration of 0.3 µg per ml. diluted by Yutao 1:3. he deposited 1 µl per droplet, and let it air dry. "48" and "72" indicate the stage of the embryos, "C" indicates the controls with the matrix proteins dialyzed against water straight from acid treatment “T” indicates samples neutralized with Tris after acid treatment and then dialyzed against water. what was the Tris pH? From Chris, the pH was 8. BSA is also in water.

Proteins on Si wafers: (2 or 3 droplets per wafer, coated with 1nm Pt) Ca movies: 48C and 48T gave type 1. 72C and 72T gave type 2 (true type 2?), however the peak 2 is small and SHIFTED. BSA gave no calcium signal at all. Ca spectra on Si.pxp Pupa thinks these spectra show Ca, not CaCO 3

Proteins on Si wafers: (2 or 3 droplets per wafer, coated with 1nm Pt) C movies: I scanned 48C and 72C only, they gave no 290.3eV spectra when the silts are 100µ and 150µ. In addition, the Ca concentration from the proteins is very low, thus the carbonate peak is not evident even if the peak exists. Pupa thinks the Ca spectra show Ca, not CaCO 3. is it true? check C spectra

Ca spectra on Calcite.pxp Proteins on calcite wafers (2 or 3 droplets per wafer, coated with 40nm+1nm Pt): Ca movies: 48C, 48T, 72C and 72T gave type2 spectra in Ca movies, no calcite, no type 1. BSA gave calcite spectra. possibly from the underlying calcite wafer?

”C spectra on Calcite.pxp” Yutao’s analysis and conclusions Proteins on calcite wafers (2 or 3 droplets per wafer, coated with 40nm+1nm Pt): C movies: 48C did not give 290.3eV peak when the slits were 100µ and 150µ (Yutao will try to check this). The other three (48T, 72C and 72T) gave 290.3eV peak when the slits were 50µ and 150µ. In addition, BSA gave a small 290.3eV peak. C spectra on calcite.pxp

Spectra comparison (48h) Calcium spectra on Si wafer from 48C and 48T

Spectra comparison (48) Calcium spectra on calcite wafer from 48C and 48T

Spectra comparison (72) Calcium spectra on Si wafer from 72C and 72T

Spectra comparison (72) Calcium spectra on calcite wafer from 72C and 72T

Spectra comparison (48C) Calcium spectra from 48C on Si and calcite wafers

Spectra comparison Calcium spectra from 72C on Si and calcite wafers

48C and 48T showed a weak Ca signal on Si wafer, but NOT a CaCO 3 (no peaks 2 and 4), then they gave type 2 spectra on calcite wafer. 72C and 72T on calcite wafer and Si wafer gave “type 2” spectra. However, the peak 2 is shifted.