RECIPE meeting Aberdeen 29-31 May 2006 Final progress report AR-WSL, EPFL ECOS Andy Siegenthaler Emanuela Samaritani, Edward Mitchell, Alexandre Buttler.

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RECIPE meeting Aberdeen May 2006 Final progress report AR-WSL, EPFL ECOS Andy Siegenthaler Emanuela Samaritani, Edward Mitchell, Alexandre Buttler

Growing keystone species experiment June 2004-July 2005 WP II A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Basal respiration WPII D20, D21, D23 A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Resumed basal respiration WPII Design plot for the CO2 production Factors CO2 production (µmol.d-1.dm-3) Low Intermed. High CT EA EV S H3 H5 H7 WTSpeciesDepth D20, D21, D23 A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Resumed basal respiration WPII Design plot for the CH4 production Factors CH4 production (umol.d-1.dm-3) Low Intermed. High CT EV EA S H3 H5 H7 WTSpeciesDepth D20, D21, D23 A.Siegenthaler, RECIPE meeting Aberdeen May 2006

WPII: Active type II methanotrophs (FISH) in growing Sphagnum (H3) A.Siegenthaler, RECIPE meeting Aberdeen May 2006 D11, D14, D20 FISH DAPI FISH/DAPI

Litter decomposition experiment August November 2005 WP III A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Litter mass-loss (% d.wt/d.wt) EAEVS Litter species EAEVSEAEVSEAEVS LowInterm.High Litter species X water level A.Siegenthaler, RECIPE meeting Aberdeen May 2006 D16, D20

In-situ surface fluxes (WPIII) CH4 flux (µmol.d-1.m-2) LowInterm.High Water level D20, D21, D23 CH4 CO2 A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Litter type effect on CO 2 and CH 4 production D20, D21, D23 A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Litter effect on basal respiration (WPIII) D20, D21, D23

Resumed basal respiration (WP III) Design plot for CO2 production Factors CO2 production (umol.d-1.dm-3) Low Interm. High CT EA EV S H3 H5 H7 WTSpeciesDepth D20, D21, D23 A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Resumed basal respiration (WP III) Design plot for CH4 production Factors CH4 production (umol.d-1.dm-3) Low Interm. High CT EAEV S H3H5 H7 WTSpeciesDepth D20, D21, D23

Resumed surface respiration WPIII Factors Design plot for CO2 production CO2 production (mmol.d-1.m2) Low Interm. High CT EA S EV Water_levelLitter_species D20, D21, D23

Resumed surface respiration WPII Design plot for CH4 production Factors CH4 production (µmol.d-1.m2) Low Intermed. High CT EA EV S Water_levelLitter_species D20, D21, D23

WPIII: Active type II methanotrophs in Sphagnum litter 0e+0 5e+5 1e+6 2e+6 3e+6 Type II Methanotrophs (ind./g f.wt) LowInterm.High Water level 0e+0 5e+6 1e+7 2e+7 DAPI (ind./g f.wt) LowInterm.High Water level A.Siegenthaler, RECIPE meeting Aberdeen May 2006 D11, D14, D20

Conclusions In a situation with low water level, the presence of any kind of litter decreases the CO2 production at -35 cm. In a situation with low water level, the presence of any kind of litter decreases the methane production at -35 cm. Under intermediate water level conditions Sphagnum litter drastically decreased the in-situ methane flux. The Sphagnum litter turnover time is higher under intermediate water level conditions. There are more methanotrophs where more methane is produced! ;-) The surface CO2 fluxes in WPIII are strongly positively correlated to the basal respiration at -15 cm depth and negatively correlated to the methanogenesis at -15 cm depth. And inversely! The surface CH4 fluxes are negatively correlated to the CO2 production at -7.5 cm depth. D11, D14, D16, D20, D21, D23 A.Siegenthaler, RECIPE meeting Aberdeen May 2006

Achievements during 2005/06 Andy (A), Emanuela (Em) Em: terminated the surface gas flux measurements (NEE, Rtot, PG) at la Chuax-d’Abel. A&Em: surface methane fluxes Chaux-d’Abel during FISH hybridisation for WPI - methodological improvement and counting of methanogens and eubacteria. Em: finished her master’s research and graduated in May A+all: WPII sampling and dispatching in July A&Em: WPIII surface fluxes, harvesting, sampling, dispatching, incubations in autumn A: WPII basal respiration for Baupt samples (monovette). A: re-analysed delta 13C-CO2 and delta 13C-CH4 of the surface samples using a supplementary cryogenic step to improve accuracy for methane. A:WPIII surface samples basal respiration for the short term decomposition experiment. A: recounting whole community in WPI with inverted microscopic (total 18 from the 27 samples. A&Em: WPII (FR): basal respiration (monovette) and FISH hybridisations for methanotrophs. A.Siegenthaler, RECIPE meeting Aberdeen May 2006