Creating Microaerophilic Atmospheric Condition for Incubation of C. jejuni Dr. Abdulaziz Bamarouf

Microaerophilic An atmosphere of 5% O 2 is appropriate for culturing C. jejuni This microaerophilic atmosphere can be produced by several ways including:

Candle Jar The oxygen concentration in a sealed jar is reduced by the burning candle and produces a microaerophilic atmosphere

Candle Jar

Evacuation Replacement System The air in a sealed jar is removed by a vacuum device and produces a microaerophilic atmosphere and then replaced with a special gas mixture

Evacuation Replacement System

Fortner Principle Campylobacter agar plate is sealed in a plastic bag with a second agar plate inoculated with E. coli Because E. coli is facultative anaerobe, it uses up some of the oxygen in the bag and produces a microaerophilic atmosphere

Fortner Principle

Gas Generating Systems The oxygen concentration in a sealed jar is reduced by using gas-generating systems Gas-generating systems are activated by adding water to a special envelope which contain tablets containing different chemicals A chemical reaction will start which consume oxygen and produces a microaerophilic atmosphere

Gas Generating Systems

Microaerophilic Bags Microaerophilic bags are commercially available Agar plate and microaerophilic gas- generating system are sealed in a disposable, gas-impermeable plastic bag Gas-generating systems are activated by crushing a special ampule

Microaerophilic Bags A chemical reaction will start which consume oxygen and produces a microaerophilic atmosphere

Anaerobic Chambers This is optimal incubation system It allows all laboratory manipulation to occur in controlled atmospheric condition Chambers equipped with airtight rubber gloves for the technologist to perform their work inside the chamber

Anaerobic Champers A= gas mixture B= entry lock F= incubator

Anaerobic Champers