Manganese Oxidizing Bacteria Cynthia McClain HMC 2012 July 19, 2012.

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Presentation transcript:

Manganese Oxidizing Bacteria Cynthia McClain HMC 2012 July 19, 2012

Mn(II) Mn(III,IV) Soluble Mn-oxide/oxyhydroxide precipitate Most MnOx in nature formed biotically!

Niche Physical and chemical gradients

Manganese Oxidizing Bacteria Multicopper oxidase or peroxidase proteins

Isolation 1.Collect water or sediment sample (slurry) 2.Streak µl supernatant on K media plate: MnCl 2, peptone, yeast extract 3.Incubate 1-3 weeks 4.Restreak brown colonies 5.Test with Leucoberbelin Blue (LBB)

Experimental Results Different morphologies – Color – Size Positive LBB – Soil – Sand from mouth of Elkhorn Slough (2) – Kirby Park oily sheen (1) – Baby seal beach sand (1)

Movie of LBB Test Sand from mouth of Elkhorn Slough

Soil

Kirby Park Oily Sheen

Baby Seal Beach

Manganese Oxidizing Bacteria Phylogeny Why oxidize Mn? Environmental Significance

Thank you!