Ongoing Standardisation of Testing for Blood-Borne Pathogens through an Internet-based Application Elizabeth M. Dax for the staff of the National Serology Reference Laboratory

NRL’s Activities Evaluations of Test Kits Quality Management Performance Monitoring Methods: QC Monitoring Specificity Batch Delivers reports and programs to generate quality in laboratories EQAS Reference and QA sample testing Testing strategy development *Education

I-Collaborations Sample characterisation Panel Distribution Sourcing samples Sample selection Sample manufacture Results collection Results analysis Reports KEY AcroMetrix HealthMetrx NRL QC EQAS Digital PT

EDCNet Internet-based QC monitoring system Laboratories submit data into ‘live’ data graphically displays data in charts instantaneous and confidential Inter- and -intralaboratory variation monitors performance of instruments, reagents and operators Facilitating a QC programme in real time NRL application (

* 2 Plasma samples

Data entered in 2005 for Selected Assays

Monitoring Precision & Accuracy S/Co Mean Mean+2SD Mean-2SD ABCDEFGHIJ

Performance of QC sample hivspy004 06/ /2003 (7 Laboratories) BatchNmean SD -2SD+2SDMinMaxCV TOTAL

Precision - CV (%) Accuracy - Bias (%) instruments testing HCV instruments testing HBsAg instruments testing HIV instruments testing HTLV Accuracy and Precision of the Abbott PRISM Bias - difference between mean ratio for individual laboratory and mean ratio for all laboratories, expressed as % of mean ratio for all laboratories

Variation by Laboratory Laboratory S/Co

Variation by Batch

Ongoing monitoring of Changes in Performance