Microbiology B.E Pruitt & Jane J. Stein AN INTRODUCTION EIGHTH EDITION TORTORA FUNKE CASE Chapter 10, part B Classification of Microorganisms

Numerical Identification Figure 10.9

Combine known antiserum + unknown bacterium Slide agglutination ELISA –Enzyme linked immunosorbent assay Western blot –Looking for different what? Serology Figure 10.10

Western Blot Figure 10.12

Phage Typing Figure 10.13

Differences in electrical conductivity between species Fluorescence of some species Cells selectively stained with antibody + fluorescent dye Flow Cytometry Uses Figure 18.11

DNA base composition –Guanine + cytosine moles% (GC) DNA fingerprinting –Electrophoresis of restriction enzyme digests RFLP rRNA sequencing Polymerase Chain Reaction (PCR) Genetics Figure 10.14

Nucleic Acid Hybridization Figure 10.15

Nucleic Acid Hybridization: DNA probe Figure 10.16

Nucleic Acid Hybridization: DNA chip Figure 10.17

Figure 10.5

Dichotomous Key

Cladogram Figure

Cladogram Figure