Phase II Study of Dasatinib in Advanced Sarcomas SARC009 Sarcoma PI: Scott Schuetze GIST PI: Jon Trent Registration and eCRF: CRAB, Seattle Drug Supply: Bristol-Myers Squibb SARC: November 13, 2008
Dasatinib Small molecule inhibitor of src-family kinases, c- kit and PDGFR Preclinical data suggested activity in Ewings & osteosarcoma in cell lines Lack of activity in PPTP pediatric tumor panel Preclinical data suggests activity in GIST kit and PDGFR mutants not responsive to imatinib
Dasatinib study objectives Primary Evaluate clinical benefit rate = Choi response or lack of progression for >6 months Secondary Evaluate 2 and 5 year survival rates Assess clinical and laboratory toxicities Collect tumor for tissue microarray Collect blood samples for drug level and functional inhibition of SRC phosphorylation
Patient Eligibility Measurable disease Age > 13 years weight > 50 kg ECOG 0-2 ANC > 1,500, Plt > 75,000 Creatinine < 2x ULN Serum calcium, magnesium and potassium > LLN Pt/PTT < 1.5 x ULN QTc interval < 450 msec LVEF > 45% (if prior treatment with anthracycline)
Exclusion/prohibitions Disease curable by multidisciplinary management Anti-platelet agents Anticoagulants Medications that prolong QT Active cardiac disease within 6 months Antacids – PPI, H-2 blockers IV bisphosphonates CYP 3A4/5 inducers/inhibitors
Treatment Plan Tumor tissue submitted to UM (mandatory – all sites) Negative pregnancy test prior to starting drug (for women of childbearing potential) CBC weekly 1 st month, then monthly Serum chemistries including magnesium monthly H&P monthly ECG baseline & after 1 st cycle Serum sample pre and post dose (selected sites) Response assessment every 2 months +/- 1 week – on time reporting of response essential
SARC009: Imaging Imaging every 8 weeks +/- 1 week, same method as baseline Target lesions at least twice the size of slice thickness on CT Target lesions on MRI should be at least 1cm Size = sum of greatest dimension of targets Density (CT only) = sum of average density of targets
Choi criteria CR = complete disappearance, no new lesions PR = >10% reduction in size or >15% decrease in density Stable = neither CR, PR or PD PD = >10% increase in size but not >15% decrease in density, or new lesions >1cm, or unequivocal progression of non-target lesions, or clinical deterioration from sarcoma
Dose Adjustment Dasatinib dosing scheme 70 mg bid starting dose 50 mg bid level -1 100 mg once daily level -2 Intolerable grade 2 event, reduce dose without interruption Significant non-hematologic grade 3 event, hold dose until grade 1 and then restart at reduced dose Grade 4 non-hematologic event, hold dose until grade 1 and then restart at reduced dose Grade 3 or 4 neutropenia or thrombocytopenia, hold dose until grade 1 and then restart at reduced dose
Correlative studies Sub-type specific tissue microarrays – stored at UM, SARC sites will have access Plasma sample obtained 2 hours after am dose 2-4 weeks after starting, store -20C or below – collection kits provided by SARC PBMC lysate from sample pre and post am dasatinib dose 2-4 weeks after starting, store -70C or lower – collection kits provided by SARC
SARC009: enrollment by site UM39 Penn21 MD Anderson11 MGH15 DFCI14 City of Hope19 Fox Chase17 Stanford17 Kootenai16 Johns Hopkins7 Indiana6 Emory8 U Pitt5 WCI4 SOC3 Nebraska2 Cedars-Sinai 2 Arkansas 1
SARC009: accrual by month
SARC009: cumulative accrual
SARC 009: “Aggressive” sub-types MFH – 34OPEN Osteosarcoma – 27On hold Leiomyosarcoma – 48CLOSED Liposarcoma – 11CLOSED Ewing’s family – 91 st stage MPNST – 51 st stage Rhabdomyosarcoma – 71 st stage N = min 9 to max 48 per stratum
SARC009: “Indolent” stratum ASPS – 2 Chordoma – 8 Conventional chondrosarcoma – 19 Epithelioid sarcoma – 3 GCT – 0 Hemangiopericytoma – 10 N = 42 (maximum 116)
SARC009: ineligible Desmoid/fibromatosis – 1 Extraskeletal myxoid chondrosarcoma - 1
SARC 009: GIST Amendment approved May 1, 2008 Imatinib resistance/intolerance +/- sunitinib 22 enrolled to date
Patient demographics Prior chemotherapy yes - 76 no - 66 missing – 54 ECOG 0 – 79 1 – 75 2 – 7 missing - 35 Age 13-25y: y: y: y: 10
Adverse events – all grades
Grade >3 AE
Dasatinib-related SAEs
Dasatinib dose
Reason off treatment Progressive disease – 88 Clinical progression – 9 Patient withdrew – 9 Death – 12 AE, not related – 6 AE, related – 3 Physician decision – 0 Other - 2
SARC009: statistical design Bayesian / dynamic analysis Start analysis after enrollment 9-10 per subtype “aggressive” subtypes - >25% response “indolent” group – 6 month PFS > 50% = promising <30% = inactive GIST - 6 month PFS >30% = promising <10% = inactive
Objective response MFH baseline 4 th cycle
Evaluable pts clinical benefit rate (CR/PR + > 6 month SD)
MFH treatment duration
LMS – treatment duration
Osteosarcoma – treatment duration
Liposarcoma – treatment duration
Progression-free survival – “indolent” & GIST
SARC009: summary Close to completing accrual in “aggressive “ sarcomas Preliminary results show activity in “MFH” Results in “indolent” sarcoma allow for continued accrual GIST too soon to tell 1/3 require dose reduction AEs: hematologic, pulmonary, GI, constitutional, pain Thanks to many investigators for rapid accrual! Thanks to SARC staff for excellent support!