CONDENSATION OF FLUORESCENT NANOPARTICLES USING A DEP CHIP WITH A DOT – ELECTRODE ARRAY STUDENT’S NAME : TRAN HONG CHUONG 陳紅章

CONTENT 1 1

INTRODUCTION 2 2

DEFINITION OF DEP (DIELECTROPHORESIS) The ability of an uncharged material to move when subjected to an electric field 3 3

DEFINITION OF MICROCHIPS Microfluidics deals with the behavior, precise control and manipulation of fluids that are geometrically constrained to a small, typically sub-millimeter, scale 4 4

DEFINITION OF FLUORESCENT Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence 5 5

SOME METHODS HAVE BEEN IMPLEMENTED BEFORE THIS PAPER 6 6

PURPOSE OF THIS PAPER DEP manipulations was considered low cost ; intrinsic electrical control ; and compability with microfluids. This paper develops a micro fluidics chip for programmable condensation of fluorescent – labeled nano particles based on DEP to enhance the strength of fluorescent density in a dot electrode array. 7 7

DEP THEORY AND NUMERICAL SIMULATION 8 8

DEP EQUATION AND EXPLAIN For a time-averaged dielectrophoretic force acting on a spherical particle immersed in a medium and exposed to a spatially non-uniform electric field. Is the electrical permittivity of the surrounding medium. Is the radius of the particle Gradient of the square of the applied electric field magnitude Particle moved particle possessing a high intensity electric field. Particle moved particle possessing a low intensity electric field. The suspend particles will not be affected by the DEP force 9 9

STRUCTURE OF DEP CHIP & SIMULATION RESULT 10

EFFECT OF BROWNIAN FORCE ON PARTICLE Is a Gaussan random vector Is a dynamic vicosity of fluid Is the boltzman’s contrast Is the temperature and T =dt is the time step 11

EFFECT OF BROWNIAN FORCE ON PARTICLE This meant that the DEP force constructed in our chip was able to sufficiently manipulate 20 nm particles and overcome the Brownian motion effects. 12

MATERIAL AND METHOD 13

SAMPLE PREPARATION In order to quantify the condensation of the NPs using DEP force, nanoparticles were labeled by fluorescent protein and observed by fluorescent optical microscopy. 14

SAMPLE PREPARATION In this experiment, we use Low-cost commercial aluminium oxide nano particles (Al2O3-NPs) 15

EXPERIMENT METHOD 16

EXPERIMENT METHOD 17

EXPERIMENTAL RESULT AND DISCUSSION 18

EXPERIMENT RESULT AND DISCUSSION 19

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MANY THANKS FOR YOUR ATTENTION!