Supplementary figure 1

Supplementary figure 1: TNF-α receptor 2 expression in the skin keratinocytes of C57 wild-type mice after TNF-α induction. (A) immunofluorescence staining of TNF- α receptor 2. (B) RT-PCR results of TNF-α receptor 2 (Lane1: treated with 0.9%NaCl; Lane 2: treated with TNF-α). Data are reported as average ± SD. *P<0.01. Bars=50µm.

Supplementary figure 2

Supplementary figure 2: Caspase-3 expression and the apoptosis in the skin of Gsdma3 mutant mice after TNF-α induction synchronized with Gsdma3 expression plasmid. (A) immunofluorescence staining of Caspase-3. (B) RT-PCR results of Caspase-3 (Lane1:treated by TNF-α; Lane 2: treated by TNF-α synchronized with Gsdma3 expression plasmid. Lane3: treated by TNF-α synchronized with empty plasmid pEGFP- N1 ). (C-D) apoptosis detected by TUNEL. (D), enlarged image of the epidermis. Data are reported as average ± SD. *P<0.01. Bars=50 µm(A,D) and 250 µm(C).

Supplementary figure 3

Supplementary figure 3 : TNF-α receptor 2 expression in the skin of Gsdma3 mutant mice. (A) immunofluorescence staining of TNF-α receptor 2 in skin keratinocytes at different stage (anagen, catagen and telogen) of hair follicle cycle. (B) immunofluorescence staining of TNF-α receptor 2 after TNF-α induction. (C) RT-PCR results of TNF-α receptor 2 after TNF-α induction (Lane1: treated with 0.9%NaCl; Lane 2: treated with TNF-α). Data are reported as average ± SD. *P<0.01. Bars=200 µm(A:anagen, catagen) and 50 µm (A:telogen, B).

Supplementary figure 4

Supplementary figure4 : TNF-α receptor 2 expression in the skin keratinocytes of C57 wild-type mice after Gsdma3 expression plasmid injection. (A) immunofluorescence staining of TNF-α receptor 2. (B) RT-PCR results of TNF-α receptor 2 (Lane1: injected with Gsdma3 expression plasmid; Lane 2: injected with empty plasmid pEGFP-N1). Data are reported as average ± SD. *P<0.01. Bars=200 µm.